Effect of light polariztion on pattern illumination super-resolution imaging

Far-field fluorescence microscopy has made great progress in the spatial resolution, limited by light diffraction, since the super-resolution imaging technology appeared. And stimulated emission depletion (STED) microscopy and structured illumination microscopy (SIM) can be grouped into one class of the super-resolution imaging technology, which use pattern illumination strategy to circumvent the diffraction limit. We simulated the images of the beads of SIM imaging, the intensity distribution of STED excitation light and depletion light in order to observe effects of the polarized light on imaging quality. Compared to fixed linear polarization, circularly polarized light is more suitable for SIM on reconstructed image. And right-handed circular polarization (CP) light is more appropriate for both the excitation and depletion light in STED system. Therefore the right-handed CP light would be the best candidate when the SIM and STED are combined into one microscope. Good understanding of the polarization will provide a reference for the patterned illumination experiment to achieve better resolution and better image quality

Fretting and mechanically-assisted crevice corrosion (MACC) of rail steel U75V with PA66 liner

Strong evidence has been reported on the occurrence of mechanically-assisted crevice corrosion (MACC) between the upper surface of the rail foot and the liner of the fastener, but there is still a lack of attention regarding its effect on materials degradation. A novel testing apparatus was self-developed in this study to simulate the MACC between the rail foot and the liner (disc-on-disc), and a typical rail steel (U75V) and a typical material for the liner (PA66) have been utilized. The fretting regimes, electrochemical behaviors and morphology of the contact surface have been investigated. It has been found that the fretting was mainly controlled by partial slip regime under the condition in this study, and the OCP and anodic current were both increased with increasing fretting frequency and displacement amplitude. The presence of PA66 debris in the form of large spalling particles has been found to inhibit crevice corrosion and relieve the surface damage to some extent. Cracks and local surface collapse were typically noticed on U75V steel which have been ascribed to the accumulation of plastic deformation and the comprehensive effect of fretting wear and corrosion fatigue

Inhibiting MARSs reduces hyperhomocysteinemia‐associated neural tube and congenital heart defects

Abstract Hyperhomocysteinemia is a common metabolic disorder that imposes major adverse health consequences. Reducing homocysteine levels, however, is not always effective against hyperhomocysteinemia‐associated pathologies. Herein, we report the potential roles of methionyl‐tRNA synthetase (MARS)‐generated homocysteine signals in neural tube defects (NTDs) and congenital heart defects (CHDs). Increased copy numbers of MARS and/or MARS2 were detected in NTD and CHD patients. MARSs sense homocysteine and transmit its signal by inducing protein lysine (N)‐homocysteinylation. Here, we identified hundreds of novel N‐homocysteinylated proteins. N‐homocysteinylation of superoxide dismutases (SOD1/2) provided new mechanistic insights for homocysteine‐induced oxidative stress, apoptosis and Wnt signalling deregulation. Elevated MARS expression in developing and proliferating cells sensitizes them to the effects of homocysteine. Targeting MARSs using the homocysteine analogue acetyl homocysteine thioether (AHT) reversed MARS efficacy. AHT lowered NTD and CHD onsets in retinoic acid‐induced and hyperhomocysteinemia‐induced animal models without affecting homocysteine levels. We provide genetic and biochemical evidence to show that MARSs are previously overlooked genetic determinants and key pathological factors of hyperhomocysteinemia, and suggest that MARS inhibition represents an important medicinal approach for controlling hyperhomocysteinemia‐associated diseases