Myrica rubra Extracts Protect the Liver from CCl4-Induced Damage

The relationship between the expression of mitochondrial voltage-dependent anion channels (VDACs) and the protective effects of Myrica rubra Sieb. Et Zucc fruit extract (MCE) against carbon tetrachloride (CCl4)-induced liver damage was investigated. Pretreatment with 50 mg kg−1, 150 mg kg−1 or 450 mg kg−1 MCE significantly blocked the CCl4-induced increase in both serum aspartate aminotransferase (sAST) and serum alanine aminotransferase (sALT) levels in mice (P < .05 or .01 versus CCl4 group). Ultrastructural observations of decreased nuclear condensation, ameliorated mitochondrial fragmentation of the cristae and less lipid deposition by an electron microscope confirmed the hepatoprotection. The mitochondrial membrane potential dropped from −191.94 ± 8.84 mV to −132.06 ± 12.26 mV (P < .01) after the mice had been treated with CCl4. MCE attenuated CCl4-induced mitochondrial membrane potential dissipation in a dose-dependent manner. At a dose of 150 or 450 mg kg−1 of MCE, the mitochondrial membrane potentials were restored (P < .05). Pretreatment with MCE also prevented the elevation of intra-mitochondrial free calcium as observed in the liver of the CCl4-insulted mice (P < .01 versus CCl4 group). In addition, MCE treatment (50–450 mg kg−1) significantly increased both transcription and translation of VDAC inhibited by CCl4. The above data suggest that MCE mitigates the damage to liver mitochondria induced by CCl4, possibly through the regulation of mitochondrial VDAC, one of the most important proteins in the mitochondrial outer membrane

Anti-cancer natural products isolated from chinese medicinal herbs

In recent years, a number of natural products isolated from Chinese herbs have been found to inhibit proliferation, induce apoptosis, suppress angiogenesis, retard metastasis and enhance chemotherapy, exhibiting anti-cancer potential both in vitro and in vivo. This article summarizes recent advances in in vitro and in vivo research on the anti-cancer effects and related mechanisms of some promising natural products. These natural products are also reviewed for their therapeutic potentials, including flavonoids (gambogic acid, curcumin, wogonin and silibinin), alkaloids (berberine), terpenes (artemisinin, β-elemene, oridonin, triptolide, and ursolic acid), quinones (shikonin and emodin) and saponins (ginsenoside Rg3), which are isolated from Chinese medicinal herbs. In particular, the discovery of the new use of artemisinin derivatives as excellent anti-cancer drugs is also reviewed

Does Cross-Border E-Commerce Promote Economic Growth? Empirical Research on China’s Pilot Zones

Whether the construction of China’s cross-border e-commerce (CBEC) comprehensive pilot zones can promote economic growth and social sustainable development is an important question worthy of discussion. This paper uses the difference-in-differences (DID) method to test the impact of the establishment of CBEC comprehensive pilot zones on economic growth and discusses the impact mechanism. The results are as follows. (1) The construction of CBEC comprehensive pilot zones can promote economic growth. After testing with parallel trend, placebo, and other robustness methods, the results are still valid. (2) The economic promotion effect of the construction of CBEC comprehensive pilot zones will be more evident in the coastal and eastern regions. The economic promotion effect of the first, second, and third batch of CBEC comprehensive pilot zones is clear. (3) The main ways that the construction of CBEC comprehensive pilot zones can facilitate economic growth are through urban digitalization, trade openness, and information service industry agglomeration

Does Cross-Border E-Commerce Promote Economic Growth? Empirical Research on China&rsquo;s Pilot Zones

Whether the construction of China&rsquo;s cross-border e-commerce (CBEC) comprehensive pilot zones can promote economic growth and social sustainable development is an important question worthy of discussion. This paper uses the difference-in-differences (DID) method to test the impact of the establishment of CBEC comprehensive pilot zones on economic growth and discusses the impact mechanism. The results are as follows. (1) The construction of CBEC comprehensive pilot zones can promote economic growth. After testing with parallel trend, placebo, and other robustness methods, the results are still valid. (2) The economic promotion effect of the construction of CBEC comprehensive pilot zones will be more evident in the coastal and eastern regions. The economic promotion effect of the first, second, and third batch of CBEC comprehensive pilot zones is clear. (3) The main ways that the construction of CBEC comprehensive pilot zones can facilitate economic growth are through urban digitalization, trade openness, and information service industry agglomeration

Agrobacterium Bioassay Strain for Ultrasensitive Detection of N-Acylhomoserine Lactone-Type Quorum-Sensing Molecules: Detection of Autoinducers in Mesorhizobium huakuii

An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations. We used this strain to detect for the first time AHLs made by Mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume Astragalus sinicus, a source of green manure throughout eastern Asia

A LuxR/LuxI-Type Quorum-Sensing System in a Plant Bacterium, Mesorhizobium tianshanense, Controls Symbiotic Nodulation

The ability of rhizobia to symbiotically fix nitrogen from the atmosphere when forming nodules on their plant hosts requires various signal transduction pathways. LuxR-LuxI-type quorum-sensing systems have been shown to be one of the players in a number of rhizobium species. In this study, we found that Mesorhizobium tianshanense, a moderate-growth Rhizobium that forms nodules on a number of licorice plants, produces multiple N-acyl homoserine lactone (AHL)-like molecules. A simple screen for AHL synthase genes using an M. tianshanense genomic expression library in Escherichia coli, coupled with a sensitive AHL detector, uncovered a LuxI-type synthase, MrtI, and a LuxR-type regulator, MrtR, in M. tianshanense. Deletions of the mrtI or mrtR locus completely abolished AHL production in M. tianshanense. Using lacZ transcriptional fusions, we found that expression of the quorum-sensing regulators is autoinduced, as mrtI gene expression requires MrtR and cognate AHLs and mrtR expression is dependent on AHLs. Compared with the wild-type strains, quorum-sensing-deficient mutants showed a marked reduction in the efficiency of root hair adherence and, more importantly, were defective in nodule formation on their host plant, Glycyrrhiza uralensis. These data provide strong evidence that quorum sensing plays a critical role in the M. tianshanense symbiotic process