Comparison of different procedures to map reference evapotranspiration using geographical information systems and regression-based techniques

16 páginas, 6 figuras, 8 tablas.This paper compares different procedures for mapping reference evapotranspiration (ETo) by means of regression-based techniques and geographical information systems (GIS). ETo is calculated following the method of Hargreaves (HG) from a dense database of meteorological stations in the northernmost semi-arid region of Europe, the Ebro valley. The HG method requires the calculation of estimates of extraterrestrial radiation (Ra). We calculated this parameter using two approaches: (1) the common approach that assumes a planar surface and determines the parameter as a function of latitude and (2) using a digital terrain model (DTM) and GIS modelling. The maps were made on a monthly basis using both approaches. We also compared possible propagations of errors in the map calculations for maps derived from modelled layers of maximum and minimum temperatures with those modelled using previously determined local ETo calculations. We demonstrate that calculations of Ra from a DTM and GIS modelling provide a more realistic spatial distribution of ETo than those derived by only considering latitude. It is also preferable to model in advance the variables involved in the calculation of ETo (temperature and Ra) and to subsequently calculate ETo by means of layer algebra in the GIS rather than directly model the local ETo calculations. The obtained maps are useful for the purposes of agriculture and ecological and water resources management in the study area.This work has been supported by the project CGL2005- 04508/BOS financed by the Spanish Comission of Science and Technology (CICYT) and FEDER, PIP176/2005 financed by the Aragón Government, and ‘Programa de grupos de investigación consolidados’ (BOA 48 of 20-04-2005), also financed by the Aragón Government. Research of the third author was supported by postdoctoral fellowship by the Ministerio de Educación, Cultura y Deporte (Spain).Peer reviewe

Micellar structure and transformations in sodium alkylbenzenesulfonate (NaLAS) aqueous solutions: effects of concentration, temperature, and salt

We investigate the shape, dimensions, and transformation pathways of micelles of linear sodium alkylbenzenesulfonate (NaLAS), a common anionic surfactant, in aqueous solution. Employing Small Angle Neutron Scattering (SANS) and surface tensiometry, we quantify the effects of surfactant concentration (0.6–15 wt%), temperature (5–40 °C) and added salt (≤0.35 M Na2SO4). Spherical micelles form at low NaLAS (≤2.6 wt%) concentration in water, and become elongated with increasing concentration and decreasing temperature. Addition of salt reduces the critical micelle concentration (CMC) and thus promotes the formation of micelles. At fixed NaLAS concentration, salt addition causes spherical micelles to grow into cylindrical micelles, and then multilamellar vesicles (MLVs), which we examine by SANS and cryo-TEM. Above a threshold salt concentration, the MLVs reach diameters of 100 s of nm to few μm, eventually causing precipitation. While the salt concentrations associated with the micelle-to-cylinder transformation increase only slightly with temperature, those required for the cylinder-to-MLV transformation exhibit a pronounced, linear temperature dependence, which we examine in detail. Our study establishes a solution structure map for this model anionic surfactant in water, quantifying the combined roles of concentration, temperature and salt, at practically relevant conditions

Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm formation

Biofilms are major causes of impairment of wound healing and patient morbidity. One of the most common and aggressive wound pathogens is Staphylococcus aureus, displaying a large repertoire of virulence factors and commonly reduced susceptibility to antibiotics, such as the spread of methicillin-resistant S. aureus (MRSA). Bacteriophages are obligate parasites of bacteria. They multiply intracellularly and lyse their bacterial host, releasing their progeny. We isolated a novel phage, DRA88, which has a broad host range among S. aureus bacteria. Morphologically, the phage belongs to the Myoviridae family and comprises a large double-stranded DNA (dsDNA) genome of 141,907 bp. DRA88 was mixed with phage K to produce a high-titer mixture that showed strong lytic activity against a wide range of S. aureus isolates, including representatives of the major international MRSA clones and coagulase-negative Staphylococcus. Its efficacy was assessed both in planktonic cultures and when treating established biofilms produced by three different biofilm-producing S. aureus isolates. A significant reduction of biofilm biomass over 48 h of treatment was recorded in all cases. The phage mixture may form the basis of an effective treatment for infections caused by S. aureus biofilms

The multiple faces of self-assembled lipidic systems

Lipids, the building blocks of cells, common to every living organisms, have the propensity to self-assemble into well-defined structures over short and long-range spatial scales. The driving forces have their roots mainly in the hydrophobic effect and electrostatic interactions. Membranes in lamellar phase are ubiquitous in cellular compartments and can phase-separate upon mixing lipids in different liquid-crystalline states. Hexagonal phases and especially cubic phases can be synthesized and observed in vivo as well. Membrane often closes up into a vesicle whose shape is determined by the interplay of curvature, area difference elasticity and line tension energies, and can adopt the form of a sphere, a tube, a prolate, a starfish and many more. Complexes made of lipids and polyelectrolytes or inorganic materials exhibit a rich diversity of structural morphologies due to additional interactions which become increasingly hard to track without the aid of suitable computer models. From the plasma membrane of archaebacteria to gene delivery, self-assembled lipidic systems have left their mark in cell biology and nanobiotechnology; however, the underlying physics is yet to be fully unraveled

Micellar structure from comparison of X-ray and neutron small-angle scattering

X-ray scattering patterns of ionic micellar solutions contain both inter- and intramicellar interferences. We show that these two terms can be separated according to the method developed by Hayter and Penfold. Both X-ray and neutron scattering signals are predicted on an absolute scale by a unique model of chain packing. Examples of results obtained through an incomplete separation of intermicellar interferences are discussed using sodium octanoate and sodium dodecylsulfate as typical examples of direct ionic micelles.Les diagrammes de diffusion aux petits angles de micelles ioniques sont constitués d'interférences intramicellaires S(q) et d'un terme intermicellaire P(q). Une méthode originale de séparation de ces deux termes dans le cas de la diffusion de neutrons a été proposée par Hayter et Penfold. Nous montrons que cette méthode permet d'interpréter pour la première fois les diagrammes de diffusion de rayons X aux petits angles de solutions concentrées de micelles. Divers résultats de la littérature sont discutés en choisissant comme exemple des solutions de dodécylsulfate (SDS) et d'octanoate (NaC8) de sodium