Aquipuribacter nitratireducens sp. nov., isolated from a soil sample of a mud volcano

A novel Gram-stain-positive, coccoid, non-motile bacterium, designated strain AMV4T, was isolated from a soil sample collected from a mud volcano located in the Andaman Islands, India. The colony was pale orange. Strain AMV4T was positive for oxidase, aesculinase, lysine decarboxylase and ornithine decarboxylase activities and negative for amylase, catalase, cellulase, protease, urease and lipase activities. 16S rRNA gene sequence analysis indicated that strain AMV4T was a member of the order Actinomycetales and was closely related to Aquipuribacter hungaricus with a sequence similarity of 97.13 % (pairwise alignment). Phylogenetic analyses showed that strain AMV4T clustered with Aquipuribacter hungaricus and was distantly related to the other genera of the family Intrasporangiaceae. DNA–DNA hybridization between strains AMV4T and Aquipuribacter hungaricus IV-75T showed a relatedness of 28 %. The predominant cellular fatty acids were iso-C15 : 0 (6.9 %), anteiso-C15 : 0 (25.3 %), C16 : 0 (12.9 %), anteiso-C16 : 0 (5.6 %), C18 : 1ω9c (19.8 %) and C18 : 3ω6,9,12c (9.1 %). The diagnostic diamino acid in the cell-wall peptidoglycan of strain AMV4T was meso-diaminopimelic acid. Strain AMV4T contained MK-10(H4) as the predominant respiratory quinone. The polar lipids consisted of phosphatidylglycerol, one unidentified glycolipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content of strain AMV4T was 74.3 mol%. Based on data from this taxonomic study using a polyphasic approach, it is proposed that strain AMV4T represents a novel species of the genus Aquipuribacter, with the suggested name Aquipuribacter nitratireducens sp. nov. The type strain is AMV4T ( = CCUG 58430T = DSM 22863T = NBRC 107137T)

Are textbook lungs really normal? A cadaveric study on the anatomical and clinical importance of variations in the major lung fissures, and the incomplete right horizontal fissure.

INTRODUCTION: The lungs have three main fissures: the right oblique fissure (ROF), right horizontal fissure (RHF), and left oblique fissure (LOF). These can be complete, incomplete or absent; quantifying the degree of completeness of these fissures is novel. Standard textbooks often refer to the fissures as complete, but awareness of variation is essential in thoracic surgery. MATERIALS AND METHODS: Fissures in 81 pairs of cadaveric lungs were classified. Oblique fissures were measured from lung hila posteriorly to the lung hila anteriorly; and the RHF measured from the ROF to the anteromedial lung edge. The degree of completeness of fissures was expressed as a percentage of the total projected length were they to be complete. The frequency and location of accessory fissures was noted. RESULTS: LOF were complete in 66/81 (81.5%), incomplete in 13/81 (16.0%) and absent in 2/81 (2.47%); ROF were complete in 52/81 (64.2%), incomplete in 29/81 (35.8%) and never absent; RHF were more variable, complete in 18/81 (22.2%), incomplete in 54/81 (66.7%) and absent in 9/81 (11.1%). LOF and ROF were on average 97.1% and 91.6% complete, respectively, being deficient posteriorly at the lung hila. The RHF on average 69.4% complete, being deficient anteromedially. There were accessory fissures in 10 left and 19 right lungs. CONCLUSIONS: This study provides a projection of the anatomy thoracic surgeons may encounter at operation, in particular the variable RHF. This knowledge is essential for optimal outcomes in both benign and oncological procedures influenced by the fissures

A Review on Failure Node Recovery Algorithms in Wireless Sensor Actor Networks

Abstract-In wireless sensor-actor networks, sensors probe their surroundings and forward their data to actor nodes. Actors collect sensor data and perform certain tasks in response to various events. Since actors operate on harsh environment, they may easily get damaged or failed. Failed actor nodes may partition the network into disjoint subsets. In order to reestablish connectivity nodes may be relocated to new positions. This paper focus on review of three (LeDir, RIM, DARA) node recovery algorithms, and their performance has been analysed in terms network overhead and path length validation metrics

Cyclobacterium qasimii sp. nov., a psychrotolerant bacterium isolated from a marine sediment of Kongsfjorden, Svalbard

A novel Gram-staining-negative, horseshoe shaped, non-motile bacterium, designated strain M12-11BT, was isolated from a marine sediment sample collected at a depth of 200 m from Kongsfjorden, Svalbard. The colony color was orangish-red due to the presence of carotenoids. The fatty acids were dominated by unsaturated fatty acids (60%), with a high abundance of C16 : 1 ω9c (30%) and C16 : 1ω11c (30%) and the other major fatty acids include anteiso-C14 : 0 (17.7%) and C15 : 0 (22%). Strain M12-11BT contained MK-4 and MK-7 as the major respiratory quinones. The polar lipids consisted of phosphatidylethanolamine (PE), one unknown phospholipid and four unknown aminolipids. Based on the 16S rRNA gene sequence similarity it was ascertained that the closest related species of M12-11BT were Cyclobacterium amurskyense, Cyclobacterium marinum and Cyclobacterium lianum with a sequence similarity of 98.2, 96.8 and 93.3% respectively. Other members of the family 'Cyclobacteriaceae' had sequence similarities of <92.0%. However, DNA-DNA hybridization with Cyclobacterium amurskyense KCTC 12363T and Cyclobacterium marinum DSM 745T showed a relatedness of only 24.5 and 32.5% with respect to strain M12-11BT. Based on the results of DNA-DNA hybridization experiments and phenotypic and chemotaxonomic data it appears that strain M12-11BT represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium qasimii sp. nov. is proposed. The G+C content of DNA of the strain M12-11BT was 40.5 mol%. The type strain of Cyclobacterium qasimii sp. nov. is M12-11BT (= KCTC 23011T = NBRC 106168T)

Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere

Three novel bacterial strains, PVAS-1<SUP>T</SUP>, B3W22<SUP>T</SUP> and B8W22<SUP>T</SUP>, were isolated from cryotubes used to collect air samples at altitudes of between 27 and 41 km. Based on phenotypic characteristics, chemotaxonomic features, DNA-DNA hybridization with the nearest phylogenetic neighbours and phylogenetic analysis based on partial 16S rRNA gene sequences (PVAS-1<SUP>T</SUP>, 1196 nt; B3W22<SUP>T</SUP>, 1541 nt; B8W22<SUP>T</SUP>, 1533 nt), the three strains were identified as representing novel species, and the names proposed are Janibacter hoylei sp. nov. (type strain PVAS-1<SUP>T</SUP> =MTCC 8307<SUP>T</SUP> =DSM 21601<SUP>T</SUP> =CCUG 56714<SUP>T</SUP>), Bacillus isronensis sp. nov. (type strain B3W22<SUP>T</SUP> =MTCC 7902<SUP>T</SUP> =JCM 13838<SUP>T</SUP>) and Bacillus aryabhattai sp. nov. (type strain B8W22<SUP>T</SUP> =MTCC 7755<SUP>T</SUP> =JCM 13839<SUP>T</SUP>)