Investigation of the association between polymorphisms of factor V (G1691A) and factor II (G20210A) with recurrent miscarriage in Iranian patients

Abstract Background: The pathogenesis of recurrent pregnancy loss includes complex interaction of several genetic and environmental factors. Changes in blood coagulation factors during pregnancy may play an important role in the occurrence of recurrent abortions (RA). Recently, inherited thrombophilia has been considered as a possible cause. Therefore, in this study we have investigated association of factor V (G1691A) and factor II (G20210A) polymorphisms in Iranian patients with recurrent abortions. Materials and Methods: A total of 203 women participated in this study: 105 women with two or more consecutive unexplained miscarriage as cases and 98 women with at least two healthy children as control group. Total genomic DNA was isolated from Peripheral blood leukocytes. The presence or absence of mutation in the FV (G1691A) and FII (G20210A) polymorphisms were assessed by PCR-RFLP, using Mnl1 and HindIII digestion enzymes, respectively. Finally, data was analyzed using Chi-Square(χ2) test. Results: The results showed no statistical significant differences in the prevalence of FV (G1691A) and FII (G20210A) polymorphisms between patients and control group. Conclusion: considering the results of this study, these polymorphisms Seem to have no role in etiology of recurrent pregnancy loss in the studied population

Association of angiotensin converting enzyme (ACE), D/I polymorphisms with recurrent pregnancy loss

Background & aim: Recurrent pregnancy loss (RPL) refers to the occurrence of two or more consecutive losses of clinically recognized pregnancies prior to the 20th week of gestation. The aim of this study was to investigate the hypothesis that recurrent pregnancy loss (RPL) is associated with a common insertion-deletion polymorphism in the angiotensin-converting enzyme gene.. Methods: The present paper intended to study the ACE deletion (D)/insertion (I) polymorphism in women with recurrent abortion. One hundred patients with recurrent abortions (at least two) as cases and one hundred healthy female with two or more normal term deliveries and without a history of abortion selected as controls. Genomic DNA was isolated from peripheral blood leukocytes and the ACE insertion/deletion (I/D) polymorphism in intron 16 was performed by polymerase chain reaction (PCR). For the statistical analysis, SPSS software version18 was used and Chi-square tests were calculated. Results: Seven patients, (7 %), and non from the control group, were homozygote (I/I) for ACE polymorphism (OR=22.82 95% CI=1.26-412.69 p=0.034), depicting no significant associations between ACE D allele or DD genotype and RPL. Conclusion: The present study showed no significant associations between ACE D allele or DD genotype and RPL