3 research outputs found
The single-cell pathology landscape of breast cancer.
Single-cell analyses have revealed extensive heterogeneity between and within human tumours1-4, but complex single-cell phenotypes and their spatial context are not at present reflected in the histological stratification that is the foundation of many clinical decisions. Here we use imaging mass cytometry5 to simultaneously quantify 35 biomarkers, resulting in 720 high-dimensional pathology images of tumour tissue from 352 patients with breast cancer, with long-term survival data availableĀ for 281 patients. Spatially resolved, single-cell analysis identified the phenotypes of tumour and stromal single cells, their organization and their heterogeneity, and enabled the cellular architecture of breast cancer tissue to be characterized on the basis of cellular composition and tissue organization. Our analysis reveals multicellular features of the tumour microenvironment and novel subgroups of breast cancer that are associated with distinct clinical outcomes. Thus, spatially resolved, single-cell analysis can characterize intratumour phenotypic heterogeneity in a disease-relevant manner, with the potential to inform patient-specific diagnosis
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Imaging mass cytometry and multiplatform genomics define the phenogenomic landscape of breast cancer
Genomic alterations shape cell phenotypes and the structure of tumor ecosystems in poorly defined ways. To investigate these
relationships, we used imaging mass cytometry to quantify the expression of 37 proteins with subcellular spatial resolution in
483 tumors from the METABRIC cohort. Single-cell analysis revealed cell phenotypes spanning epithelial, stromal and immune
types. Distinct combinations of cell phenotypes and cellācell interactions were associated with genomic subtypes of breast
cancer. Epithelial luminal cell phenotypes separated into those predominantly impacted by mutations and those affected by
copy number aberrations. Several features of tumor ecosystems, including cellular neighborhoods, were linked to prognosis,
illustrating their clinical relevance. In summary, systematic analysis of single-cell phenotypic and spatial correlates of genomic
alterations in cancer revealed how genomes shape both the composition and architecture of breast tumor ecosystems and will
enable greater understanding of the phenotypic impact of genomic alterations
Breast tumor microenvironment structures are associated with genomic features and clinical outcome.
The functions of the tumor microenvironment (TME) are orchestrated by precise spatial organization of specialized cells, yet little is known about the multicellular structures that form within the TME. Here we systematically mapped TME structures in situ using imaging mass cytometry and multitiered spatial analysis of 693 breast tumors linked to genomic and clinical data. We identified ten recurrent TME structures that varied by vascular content, stromal quiescence versus activation, and leukocyte composition. These TME structures had distinct enrichment patterns among breast cancer subtypes, and some were associated with genomic profiles indicative of immune escape. Regulatory and dysfunctional T cells co-occurred in large 'suppressed expansion' structures. These structures were characterized by high cellular diversity, proliferating cells and enrichment for BRCA1 and CASP8 mutations and predicted poor outcome in estrogen-receptor-positive disease. The multicellular structures revealed here link conserved spatial organization to local TME function and could improve patient stratification