Measuring Lattice Strain in Three Dimensions through Electron Microscopy

The three-dimensional (3D) atomic structure of nanomaterials, including strain, is crucial to understand their properties. Here, we investigate lattice strain in Au nanodecahedra using electron tomography. Although different electron tomography techniques enabled 3D characterizations of nanostructures at the atomic level, a reliable determination of lattice strain is not straightforward. We therefore propose a novel model-based approach from which atomic coordinates are

Comparative validation of recent 10 m-resolution global land cover maps

Accurate and high-resolution land cover (LC) information is vital for addressing contemporary environmental challenges. With the advancement of satellite data acquisition, cloud-based processing, and deep learning technology, high-resolution Global Land Cover (GLC) map production has become increasingly feasible. With a growing number of available GLC maps, a comprehensive evaluation and comparison is necessary to assess their accuracy and suitability for diverse uses. This particularly applies to maps lacking statistically robust accuracy assessment or sufficient reported detail on the validation procedures. This study conducts a comparative independent validation of recent 10 m GLC maps, namely ESRI Land Use/Land Cover (LULC), ESA WorldCover, and Google and World Resources Institute (WRI)’s Dynamic World, examining their spatial detail representation and thematic accuracy at global, continental, and national (for 47 larger countries) levels. Since high-resolution map validation is impacted by reference data uncertainty owing to geolocation and labelling errors, five validation approaches dealing with reference data uncertainty were evaluated. Of the considered approaches, validation using the sample label supplemented by majority label within the neighborhood is found to produce more reasonable accuracy estimates compared to the overly optimistic approach of using any label within the neighborhood and the overly pessimistic approach of direct comparison between the map and reference labels. Overall global accuracies of the maps range between 73.4% ± 0.7% (95% confidence interval) to 83.8% ± 0.4% with WorldCover having the highest accuracy followed by Dynamic World and ESRI LULC. The quality of the maps varies across different LC classes, continents, and countries. The maps' spatial detail representation was assessed at various homogeneity levels within a 3 × 3 kernel. Although considered as high-resolution maps, this study reveals that ESRI LULC and Dynamic World have less spatial detail than WorldCover. All maps have lower accuracies in heterogenous landscapes and in some countries such as Mozambique, Tanzania, Nigeria, and Spain. To select the most suitable product, users should consider both the map's accuracy over the area of interest and the spatial detail appropriate for their application. For future high-resolution GLC mapping, producers are encouraged to adopt standardized LC class definitions to ensure comparability across maps. Additionally, the spatial detail and accuracy of GLC maps in heterogeneous landscapes and over some countries are the key features that should be improved in future versions of the maps. Independent validation efforts at regional and national levels, as well as for LC changes, should be strengthened to enhance the utility of GLC maps at these scales and for long-term monitoring

WorldCereal: a dynamic open-source system for global-scale, seasonal, and reproducible crop and irrigation mapping

The challenge of global food security in the face of population growth, conflict and climate change requires a comprehensive understanding of cropped areas, irrigation practices and the distribution of major commodity crops like maize and wheat. However, such understanding should preferably be updated at seasonal intervals for each agricultural system rather than relying on a single annual assessment. Here we present the European Space Agency funded WorldCereal system, a global, seasonal, and reproducible crop and irrigation mapping system that addresses existing limitations in current global-scale crop and irrigation mapping. WorldCereal generates a range of global products, including temporary crop extent, seasonal maize and cereals maps, seasonal irrigation maps, seasonal active cropland maps, and confidence layers providing insights into expected product quality. The WorldCereal product suite for the year 2021 presented here serves as a global demonstration of the dynamic open-source WorldCereal system. The presented products are fully validated, e.g., global user's and producer's accuracies for the annual temporary crop product are 88.5 % and 92.1 %, respectively. The WorldCereal system provides a vital tool for policymakers, international organizations, and researchers to better understand global crop and irrigation patterns and inform decision-making related to food security and sustainable agriculture. Our findings highlight the need for continued community efforts such as additional reference data collection to support further development and push the boundaries for global agricultural mapping from space. The global products are available at https://doi.org/10.5281/zenodo.7875104 (Van Tricht et al., 2023)

Serological response and breakthrough infection after COVID-19 vaccination in patients with cirrhosis and post-liver transplant

Background: Vaccine hesitancy and lack of access remain major issues in disseminating COVID-19 vaccination to liver patients globally. Factors predicting poor response to vaccination and risk of breakthrough infection are important data to target booster vaccine programs. The primary aim of the current study was to measure humoral responses to 2 doses of COVID-19 vaccine. Secondary aims included the determination of factors predicting breakthrough infection. Methods: COVID-19 vaccination and Biomarkers in cirrhosis And post-Liver Transplantation is a prospective, multicenter, observational case-control study. Participants were recruited at 4-10 weeks following first and second vaccine doses in cirrhosis [n = 325; 94% messenger RNA (mRNA) and 6% viral vaccine], autoimmune liver disease (AILD) (n = 120; 77% mRNA and 23% viral vaccine), post-liver transplant (LT) (n = 146; 96% mRNA and 3% viral vaccine), and healthy controls (n = 51; 72% mRNA, 24% viral and 4% heterologous combination). Serological end points were measured, and data regarding breakthrough SARS-CoV-2 infection were collected. Results: After adjusting by age, sex, and time of sample collection, anti-Spike IgG levels were the lowest in post-LT patients compared to cirrhosis (p < 0.0001), AILD (p < 0.0001), and control (p = 0.002). Factors predicting reduced responses included older age, Child-Turcotte-Pugh B/C, and elevated IL-6 in cirrhosis; non-mRNA vaccine in AILD; and coronary artery disease, use of mycophenolate and dysregulated B-call activating factor, and lymphotoxin-α levels in LT. Incident infection occurred in 6.6%, 10.6%, 7.4%, and 15.6% of cirrhosis, AILD, post-LT, and control, respectively. The only independent factor predicting infection in cirrhosis was low albumin level. Conclusions: LT patients present the lowest response to the SARS-CoV-2 vaccine. In cirrhosis, the reduced response is associated with older age, stage of liver disease and systemic inflammation, and breakthrough infection with low albumin level

Clinical features and comorbidity pattern of HCV infected migrants compared to native patients in care in Italy: A real-life evaluation of the PITER cohort

Background: Direct-acting antivirals are highly effective for the treatment of hepatitis C virus (HCV) infection, regardless race/ethnicity. We aimed to evaluate demographic, virological and clinical data of HCV-infected migrants vs. natives consecutively enrolled in the PITER cohort. Methods: Migrants were defined by country of birth and nationality that was different from Italy. Mann-Whitney U test, Chi-squared test and multiple logistic regression were used. Results: Of 10,669 enrolled patients, 301 (2.8%) were migrants: median age 47 vs. 62 years, (p &lt; 0.001), females 56.5% vs. 45.3%, (p &lt; 0.001), HBsAg positivity 3.8% vs. 1.4%, (p &lt; 0.05). Genotype 1b was prevalent in both groups, whereas genotype 4 was more prevalent in migrants (p &lt; 0.05). Liver disease severity and sustained virologic response (SVR) were similar. A higher prevalence of comorbidities was reported for natives compared to migrants (p &lt; 0.05). Liver disease progression cofactors (HBsAg, HIV coinfection, alcohol abuse, potential metabolic syndrome) were present in 39.1% and 47.1% (p &gt; 0.05) of migrants and natives who eradicated HCV, respectively. Conclusion: Compared to natives, HCV-infected migrants in care have different demographics, HCV genotypes, viral coinfections and comorbidities and similar disease severity, SVR and cofactors for disease progression after HCV eradication. A periodic clinical assessment after HCV eradication in Italians and migrants with cofactors for disease progression is warranted

Gender Differences in COVID-19 Among Liver Transplant Recipients: Results from a Multicenter Brazilian Cohort

Introduction: Existing literature presents varying perspectives on the impact of COVID-19 on liver transplant recipients.However, no research has specifically investigated the role of gender differences in the manifestation of COVID-19 among liver transplant recipients. This study aims to examine the effects of COVID-19 on liver transplant recipients, with a focus on gender differences in disease presentation and progression. Methods: Conducted as a multicenter historical cohort study, this research collected patient records through an online questionnaire. Assessing COVID-related mortality was the main objective. Additionally, demographic, clinical, and laboratory data pertaining to disease presentation and progression werecollected. Results: The study included a total of 283 patients, of whom 76 were female and 206 were male. The median follow-up period for males was 99 days (IQR 38-283), while for females, it was 126 days (IQR 44-291). A higher prevalence of cardiovascular disease was observed in males (p=0.002). Females frequently experienced a loss of smell (p=0.021), whereas males commonly exhibited fever (p=0.031). Levels of ALT and gamma-glutamyl transferase were significantly elevated in males (p=0.008 and 0.004, respectively). Although there was a trend towards increased mortality in males, it did not reach statistical significance. Conclusion: This study is the first attempt to investigate gender differences in COVID-19 among liver transplant recipients. Our findings highlight the need for a comprehensive and personalised approach to treating this patient population and underscore the importance of further elucidating the disease presentation in these individuals

High resolution and high cadence time series of land surface categories, land use land cover, and land use land cover changes

A prototype of monthly, 10 m resolution land surface categories, land use land cover (LULC) cover, and LULC change maps derived from Sentinel-2 data over three areas within Belgium, Portugal, and Sicily for the period 2018-2020. The LULC and LULC change maps were independently validated by IIASA. All products were generated within the framework of the RapidAI4EO project, funded from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 101004356. The data description can be found below. The validation report of the LULC and LULC change maps can be found in validation_LULC.pdf and validation_change.pdf, respectively, and the validation dataset can be found in Lesiv et al. (2023). Data description Increasing the cadence of the land cover updates from the typical (multi-)annual to monthly cadence poses several challenges. First, several land cover types are difficult to discriminate without any knowledge of temporal dynamics. For instance, croplands are characterized by a dynamic of vegetation growth and a harvest period (i.e. cycles of bare soil, sparsely vegetated and vegetated periods). This contrasts with grasslands that often lack the harvest period resulting in a bare soil cover. Without this temporal information, it is difficult to distinguish a vegetated cropland field from grassland. Second, phenological changes may introduce a large intra-class variability and thus also confusion between classes. For example, the shedding of leaves during autumn or wilting of herbaceous vegetation in dry summer periods introduces spectral variability within land cover classes. To overcome these challenges, we developed a workflow with two main phases. The first phase aims to map land surface categories (LSC) at a monthly resolution. The next phase uses the resulting monthly LSC probability time series to classify land cover

Quantitative 3D analysis of huge nanoparticle assemblies

Nanoparticle assemblies can be investigated in 3 dimensions using electron tomography. However, it is not straightforward to obtain quantitative information such as the number of particles or their relative position. This becomes particularly difficult when the number of particles increases. We propose a novel approach in which prior information on the shape of the individual particles is exploited. It improves the quality of the reconstruction of these complex assemblies significantly. Moreover, this quantitative Sparse Sphere Reconstruction approach yields directly the number of particles and their position as an output of the reconstruction technique, enabling a detailed 3D analysis of assemblies with as many as 10 000 particles. The approach can also be used to reconstruct objects based on a very limited number of projections, which opens up possibilities to investigate beam sensitive assemblies where previous reconstructions with the available electron tomography techniques failed