Evaluation of the effect of different insecticides on the survival and capacity of 2 Eretmocerus mundus Mercet to control Bemisia tabaci (Gennadius) populations

Two different experiments were carried out to evaluate three insecticides. In the 24 first one, the effect of two insecticides, methomyl and indoxacarb, on pupae and 25 adults of the whitefly Bemisia tabaci (Gennadius) parasitoid Eretmocerus mundus 26 Mercet was evaluated under laboratory and greenhouse conditions, using sweet 27 pepper (Capsicum annuum L.) plants. In the second experiment, oxamyl was 28 tested to study its effect on the ability of E. mundus to parasitize and control B. 29 tabaci in sweet pepper plants, using a greenhouse cage evaluation. Methomyl and 30 indoxacarb caused low mortality of E. mundus pupae (17.6 and 7.8% 31 respectively), although methomyl mortality was significantly higher. Methomyl 32 produced 100% mortality on E. mundus adults with fresh and 24 hour-old 33 residues on leaves, significantly higher than the mortality produced by indoxacarb 34 (values ranged from 43.9 to 34.4%). The harmful effect of methomyl persisted for 35 a long time (up to 60 days). The results of the experiment with oxamyl showed 36 that E. mundus controlled whitefly population, without significant interaction 37 between the presence of the parasitoid and insecticide on whitefly mortality. 38 Whitefly mortality in the presence of the parasitoid was 87.8%, significantly higher 39 than the mortality in the absence of E. mundus (59.3%). Oxamyl did not produce 40 a significant effect on the emergence of E. mundus adults. Application of the 41 products in IPM programs is discussed

Subglacial lakes and hydrology across the Ellsworth Subglacial Highlands, West Antarctica

Subglacial water plays an important role in ice sheet dynamics and stability. Subglacial lakes are often located at the onset of ice streams and have been hypothesised to enhance ice flow downstream by lubricating the ice– bed interface. The most recent subglacial-lake inventory of Antarctica mapped nearly 400 lakes, of which ∼ 14 % are found in West Antarctica. Despite the potential importance of subglacial water for ice dynamics, there is a lack of detailed subglacial-water characterisation in West Antarctica. Using radio-echo sounding data, we analyse the ice–bed interface to detect subglacial lakes. We report 33 previously uncharted subglacial lakes and present a systematic analysis of their physical properties. This represents a ∼ 40 % increase in subglacial lakes in West Antarctica. Additionally, a new digital elevation model of basal topography of the Ellsworth Subglacial Highlands was built and used to create a hydropotential model to simulate the subglacial hydrological network. This allows us to characterise basal hydrology, determine subglacial water catchments and assess their connectivity. We show that the simulated subglacial hydrological catchments of the Rutford Ice Stream, Pine Island Glacier and Thwaites Glacier do not correspond to their ice surface catchments

Global methylome scores correlate with histological subtypes of colorectal carcinoma and show different associations with common clinical and molecular features

Background. The typical methylation patterns associated with cancer are hypermethylation at gene promoters and global genome hypomethylation. Aberrant CpG island hypermethylation at promoter regions and global genome hypomethylation have not been associated with histological colorectal carcinomas (CRC) subsets. Using Illumina’s 450 k Infinium Human Methylation beadchip, the methylome of 82 CRCs were analyzed, comprising different histological subtypes: 40 serrated adenocarcinomas (SAC), 32 conventional carcinomas (CC) and 10 CRCs showing histological and molecular features of microsatellite instability (hmMSI‐H), and, additionally, 35 normal adjacent mucosae. Scores reflecting the overall methylation at 250 bp, 1 kb and 2 kb from the transcription starting site (TSS) were studied. Results. SAC has an intermediate methylation pattern between CC and hmMSI‐H for the three genome locations. In addition, the shift from promoter hypermethylation to genomic hypomethylation occurs at a small sequence between 250 bp and 1 Kb from the gene TSS, and an asymmetric distribution of methylation was observed between both sides of the CpG islands (N vs. S shores). Conclusion. These findings show that different histological subtypes of CRC have a particular global methylation pattern depending on sequence distance to TSS and highlight the so far underestimated importance of CpGs aberrantly hypomethylated in the clinical phenotype of CRCs.This work was supported by two grants from Instituto de Salud Carlos III, Ministerio de Sanidad, Spain and FEDER funds (refs: PI12-1232, PI18-0144) and another from the European Union’s Horizon 2020 research and innovation program (ref. 848098

Spanish guidelines for the use of targeted deep sequencing in myelodysplastic syndromes and chronic myelomonocytic leukaemia

The landscape of medical sequencing has rapidly changed with the evolution of next generation sequencing (NGS). These technologies have contributed to the molecular characterization of the myelodysplastic syndromes (MDS) and chronic myelomonocytic leukaemia (CMML), through the identification of recurrent gene mutations, which are present in >80% of patients. These mutations contribute to a better classification and risk stratification of the patients. Currently, clinical laboratories include NGS genomic analyses in their routine clinical practice, in an effort to personalize the diagnosis, prognosis and treatment of MDS and CMML. NGS technologies have reduced the cost of large-scale sequencing, but there are additional challenges involving the clinical validation of these technologies, as continuous advances are constantly being made. In this context, it is of major importance to standardize the generation, analysis, clinical interpretation and reporting of NGS data. To that end, the Spanish MDS Group (GESMD) has expanded the present set of guidelines, aiming to establish common quality standards for the adequate implementation of NGS and clinical interpretation of the results, hoping that this effort will ultimately contribute to the benefit of patients with myeloid malignancies

The role of retinal fluid location in atrophy and fibrosis evolution of patients with neovascular age-related macular degeneration long-term treated in real world

Purpose: To assess the effect of clinical factors on the development and progression of atrophy and fibrosis in patients with neovascular age-related macular degeneration (nAMD) receiving long-term treatment in the real world. Methods: An ambispective 36-month multicentre study, involving 359 nAMD patients from 17 Spanish hospitals treated according to the Spanish Vitreoretinal Society guidelines, was designed. The influence of demographic and clinical factors, including the presence and location of retinal fluid, on best-corrected visual acuity (BCVA) and progression to atrophy and/or fibrosis were analysed. Results: After 36 months of follow-up and an average of 13.8 anti-VEGF intravitreal injections, the average BCVA gain was +1.5 letters, and atrophy and/or fibrosis were present in 54.8% of nAMD patients (OR = 8.54, 95% CI = 5.85-12.47, compared to baseline). Atrophy was associated with basal intraretinal fluid (IRF) (OR = 1.87, 95% CI = 1.09-3.20), whereas basal subretinal fluid (SRF) was associated with a lower rate of atrophy (OR = 0.40, 95% CI = 0.23-0.71) and its progression (OR = 0.44, 95% CI = 0.26-0.75), leading to a slow progression rate (OR = 0.34, 95% CI = 0.14-0.83). Fibrosis development and progression were related to IRF at any visit (p < 0.001). In contrast, 36-month SRF was related to a lower rate of fibrosis (OR = 0.49, 95% CI = 0.29-0.81) and its progression (OR = 0.50, 95% CI = 0.31-0.81). Conclusion: Atrophy and/or fibrosis were present in 1 of 2 nAMD patients treated for 3 years. Both, especially fibrosis, lead to vision loss. Subretinal fluid (SRF) was associated with good visual outcomes and lower rates of atrophy and fibrosis, whereas IRF yields worse visual results and a higher risk of atrophy and especially fibrosis in routine clinical practice

Caracterización de la pandemia de gripe A H1N1 2009 en Navarra

Fundamento. Describir la actividad gripal durante la pandemia de 2009-2010 en Navarra y compararla con la de temporadas anteriores. Métodos. Se han analizado los casos de gripe notificados en atención primaria y todas las confirmaciones virológicas realizadas en pacientes de atención primaria y en hospitales de Navarra entre las semanas 21 de 2009 y 20 de 2010. Resultados. El virus de la gripe A (H1N1) 2009 se detectó en Navarra entre las semana 23 de 2009 a la 2 de 2010, periodo en el que se registraron 39 casos con diagnóstico médico de síndrome gripal por 1.000 habitantes. El umbral epidémico se superó en dos periodos, con un pico en julio y otro mayor en noviembre. La mayor incidencia se alcanzó en niños de 5 a 14 años (121 por mil), seguidos por el grupo de menores de 5 años. Se produjeron 224 hospitalizaciones (36 por 100.000 habitantes) con confirmación de gripe A H1N1 2009, 8% de ellos requirieron ingreso en unidades de cuidados intensivos y hubo cuatro defunciones (0,6 por 100.000 habitantes). La tasa de hospitalizaciones fue mayor en niños menores de 5 años (163 por 100.000 habitantes), mientras que la probabilidad de derivación a cuidados intensivos aumentó con la edad. Conclusión. A pesar de no haber dispuesto de una vacuna específica hasta que la temporada estaba muy avanzada, el virus de gripe A (H1N1) 2009 produjo una onda gripal en rangos similares a los de otras temporadas y su repercusión en hospitalizaciones y casos graves fue moderada.Background. To describe influenza activity during the 2009-2010 pandemic in Navarre and compare it to previous seasons. Methods. An analysis was made of all influenza-like illness cases reported in primary care and all the virological confirmations made in patients in primary care and in hospitals of Navarre between week 21 of 2009 and week 20 of 2010. Results. Influenza 2009 H1N1 virus was detected in Navarre between week 23 of 2009 and week 2 of 2010, a period when 39 medically diagnosed cases of influenza-like illness per 1,000 inhabitants were registered. The epidemic threshold was surpassed in two periods, with a peak in July and a greater one in November. The greatest incidence was reached in children aged between 5 and 14 years (121 per thousand), followed by the group of under fives. There were 224 hospitalisations (36 per 100,000 inhabitants) with confirmation of influenza 2009 H1N1 virus, 8% of whom required admission to intensive care units and there were four deaths (0.6 per 100,000 inhabitants). The rate of hospitalisation was greater amongst children under five (163 per 100,000 inhabitants), while the probability of referral to intensive care increased with age. Conclusion. In spite of not having a specific vaccine available until the season was very well advanced, influenza 2009 H1N1 virus produced a wave of cases with similar incidence to those of other seasons and its repercussion in hospitalisations and serious cases was moderate

Growth dynamics and yield of melon as influenced by nitrogen fertilizer

Nitrogen (N) is an important nutrient for melon (Cucumis melo L.) production. However there is scanty information about the amount necessary to maintain an appropriate balance between growth and yield. Melon vegetative organs must develop sufficiently to intercept light and accumulate water and nutrients but it is also important to obtain a large reproductive-vegetative dry weight ratio to maximize the fruit yield. We evaluated the influence of different N amounts on the growth, production of dry matter and fruit yield of a melon 'Piel de sapo' type. A three-year field experiment was carried out from May to September. Melons were subjected to an irrigation depth of 100% crop evapotranspiration and to 11 N fertilization rates, ranging 11 to 393 kg ha-1 in the three years. The dry matter production of leaves and stems increased as the N amount increased. The dry matter of the whole plant was affected similarly, while the fruit dry matter decreased as the N amount was increased above 112, 93 and 95 kg ha-1, in 2005, 2006 and 2007, respectively. The maximum Leaf Area Index (LAI), 3.1, was obtained at 393 kg ha-1 of N. The lowest N supply reduced the fruit yield by 21%, while the highest increased the vegetative growth, LAI and Leaf Area Duration (LAD), but reduced yield by 24% relative to the N93 treatment. Excessive applications of N increase vegetative growth at the expense of reproductive growth. For this melon type, rates about 90-100 kg ha-1 of N are sufficient for adequate plant growth, development and maximum production. To obtain fruit yield close to the maximum, the leaf N concentration at the end of the crop cycle should be higher than 19.5 g kg-1.O nitrogênio (N) é um nutriente importante para a produção de melão (Cucumis melo L.), porém existe pouca informação sobre a quantidade necessária para se obter um balanço entre o crescimento e a produtividade. Os órgãos vegetativos do melão precisam se desenvolver suficientemente para interceptor luz, acumular água e nutrients, mas também é importante alcançar uma grande relação de massa seca produtiva-vegetativa para maximizar a produção de frutos. Investigou-se a influência de quantidades de N no crescimento, na produção de matéria seca e na produtividade do melão tipo 'Pele de sapo'. Foi conduzido experimento com três anos de duração, de maio a setembro, com irrigações de 100% da evapotranspiração e 11 doses de adubação de N, no intervalo de 11 a 393 kg ha-1 em três anos. A produção de massa seca de folhas e caules aumentou com o aumento das doses de N. A matéria seca da planta toda foi afetada de maneira semelhante, enquanto a dos frutos decresceu com o aumento de N acima de 112, 93 e 95 kg ha-1, em 2005, 2006 e 2007, respectivamente. O índice de área foliar máxima (LAI) mais aulto (3.1) foi obtido com a dose de 393 kg N ha-1de N. A dose mais baixa de N reduziu a produtividade de frutos em 21%, enquanto a dose mais alta aumentou o crescimento vegetative, LAI e a duração de área foliar (LAD), mas reduziu a produtividade em 24% em relação ao tratamento N93. Aplicações excessivas de N aumentam o crescimento vegetativo às expensas do crescimento vegetativo. Para este tipo de melão, doses da ordem de 90-100 kg ha-1 de N são suficientes para crescimento adequado e produção máxima. Para obter produções próximas ao máximo, a concentração de N na folha no final do ciclo da cultura não deve ser maior que 19.5 g kg-1

Unique clinico-biological, genetic and prognostic features of adult early T-cell precursor acute lymphoblastic leukemia

Altres ajuts: this project was supported by the Asociación Española Contra el Cáncer (project ref: GC16173697BIGA), [...], and Obra Social "La Caixa"