POLYMORPHISM OF GSTM1 AND GSTT1 GENES IN WORKERS OF FERROUS METALLURGY WITH PROFESSIONAL FLUOROSIS

The aim of the present work was to study genetic risk factors for the development of professional fluorosis in workers of Novokuznetsk aluminum factory, the presence of which is associated with the risk of professional dental fluorosis and resistance to it. The study involved 79 male workers of Novokuznetsk aluminum factory Ltd. "RUSAL" of 35-60 years. The control group (212 people) consisted of healthy individuals working for the same company and without fluorosis. The material for the study were the venous blood samples. Polymorphism of null-alleles of genes encoding transferase phase 2 biotransformation of xenobiotics - GSTT1 and GSTM1 was studied. In this study DNA was isolated by phenolhloroformnym method performing polymerase chain reaction amplification products electrophoresed on agarose gel, and the products were visualized under ultraviolet light. The frequencies of alleles and genotypes in groups of patients and healthy subjects were compared using the χ2 test with Yates correction for continuity. The association of different genotypes (or combinations thereof) was estimated by the magnitude of the disease odds ratio (OR). The frequencies of alleles and phenotypes in our sample were studied on an aluminum factory workers and were already comparable to previously published data on Russian Federation and Siberian federal district. Analysis of differences in genotype frequencies between patients with fluorosis and those of the control group using χ2 criteria and OR shows that the largest contribution to the risk of developing the disease is making by null-allele of the GSTT1 gene (GSTT1(-)), as well as the "double zero" - a combination of GSTT1(-) and GSTM1(-). On the other hand the holders GSTT1(+)) as well as combinations of genotypes GSTT1(+) / GSTM1(-) are the most resistant to this disease genotype. The obtained data can be used in the formation of risk groups for development of fluorosis, for timely diagnosis and prevention of loss of workers' health

METHOD FOR THE PREDICTION OF THE DEVELOPMENT OF CHRONIC PULMONARY HEART IN COAL WORKERS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

452 miners at the age of40-55years (average age is 50,9 ± 0,2 years) with chronic obstructive pulmonary disease were studied. Chronic pulmonary heart was detected in 289 patients (63,9 %). Using Bayes' method based on the determination of prognostic factors we have elaborated the technique for the prediction of the development of chronic pulmonary heart in miners with chronic obstructive pulmonary disease. The technique is based on the presence of respiratory insufficiency, associated ischemic heart disease and arterial hypertension, blood groups by AB0, MN, and P systems, some structural and functional indicators of heart (a myocardium mass index of aortic ventricle heart, an index of relative thickness of walls of aortic ventricle heart, an index of sphericity of aortic ventricle heart and an average pressure in a pulmonary artery). The use of genetic markers of blood groups by AB0, MN, and P systems allows more detailed estimate genetic predisposition to disease. By increasing the number of analyzed factors influencing the development of chronic pulmonary heart and using of additional markers, we have more accurate prediction of this complication. All figures are reduced in the general prognostic table. The work with the table is the following, it is necessary to sum up all the prognostic coefficients taking into account a sign. At the value of total amount of+6 points or more predisposition to development of chronic pulmonary heart for a worker is more predicted, at the sum less than -6 points we predict the resistance to development of chronic pulmonary heart, at intermediate values the forecast is not defined. The main advantage of the technique is speed and minor material costs. So, while conducting a single assessment of the health of miners with chronic obstructive pulmonary disease in outpatient and polyclinic conditions it is simply to select a group of persons at high risk of developing chronic pulmonary heart for timely preventive actions

SEXUALLY TRANSMITTED INFECTIONS AS THE PROBLEM OF DEMOGRAPHIC DEVELOPMENT OF SIBERIAN FEDERAL DISTRICT

In the article the negative medico-demographic and social and economic value of consequences of sexually transmitted infections is indicated that causes their inclusion in the list of socially significant diseases which constitute danger to people around. It is specified that complications of sexually transmitted infections lead to formation of congenital diseases, break reproductive function of younger generation, negatively affect birth rate indicators, worsen a demographic situation, aggravating depopulation processes in Russia and its territorial units. The most significant in this respect is the disease of syphilis. The dynamic assessment of the incidence rate of syphilis in the Russian Federation, the Siberian Federal District and the Kemerovo Region in the period from 2006 to 2011 shows that as a whole across Russia incidence of syphilis in the last 5years has a tendency to decrease, in different degree expressed in the territorial units. Less favorable trends in the dynamics of syphilis in the Kemerovo Region in comparison with the Siberian Federal District and the Russian Federation are revealed. Namely, if during the period from 2006 to 2008, the differences between the indices of the Kemerovo Region and the Siberian Federal District held at the level of 7%; while in 2009 the difference increased to 12%, and in 2011 reached 25% (not in favor of the Kemerovo region). So, in 2011 indicator level in the Kemerovo region was 2.36 times higher than in the Russian Federation as a whole. Taking into account the emphasized medico-demographic importance of sexually transmitted infections and the expressed distinctions in the values of analyzed indicators, it is shown that the Kemerovo Region is the priority territory for the development of measures aimed at reducing the incidence of syphilis in the spectrum of approaches to improve the demographic policy Siberian Federal District

THE SCIENTIFIC REVIEW OF THE RESULTS OF RESEARCH INSTITUTE FOR COMPLEX PROBLEMS OF HYGIENE AND OCCUPATIONAL DISEASES SB RAMS RESEARCHES ON The INFLUENCE OF ENVRONMENTAL And GENETIC FACTORS ON THE DEVELOPMENT OF OCCUPATIONAL DISEASES

On the main scientific directions of "RI CPHOD" SB RAMS connected with the study on the bases of human reproductive health formation estimation, and prediction of population reproduction and. losses of health in the regions of Siberia, the Far East and. the Far North; the hygienic estimation of social, ecological and. industrial conditions in the territories of Siberia with the elaboration of effective methods for prevention, treatment and. rehabilitation of occupational diseases, industrially caused diseases and. common somatic diseases in the working communities; the study on the problems of management and. organization of public health care systems, elaboration of methodological bases and. techniques for formation of strategy and. technology for realization of health improving programs, territorial public health services, medical services and. organizations the scientific review of the results of the researches of the influence of environmental and. genetic factors on the development, course and. outcomes of occupational diseases is carried out

PECULIARITIES OF SILICOTUBERCULOSIS IN THE WORKERS OF FERROUS METALLURGY ENTERPRISES

The results of long-term dispensary supervision (14 years) of 284 silicosis patients (including 54 silicotuberculosis patients) worked at Kuznetsk Metallurgical Plant have shown that high concentration of dust in air with 5% content of free silicon dioxide promotes the development of non-complicated silicosis as well as silicotuberculosis. This dispensary supervision, has been conducted by the physicians of scientific and consultative department of FSBI "RI CPHOD" SB RAMS in Novokuznetsk. Fast development of silicosis is risk factor for silicotuberculosis (р < 0,05). Slow development of silicosis is much rarely associated by tuberculosis infection. The patients with non-complicated. silicosis and. silicotuberculosis have the signs of pneumonia (oftener in non-complicated silicosis) and. chronic obstructive pulmonary disease (oftener in silicotuberculosis) according to the results of chest radiography

To the necessity of palliative care of municipal level in the conditions of the regional subordination of oncology service (on the example of Novokuznetsk, Kemerovo region)

It is emphasized that according to the WHO cancer is one of the leading causes of death in the world. The dynamic evaluation of the primary incidence rate of malignant neoplasms among the inhabitants of Novokuznetsk, Kemerovo Region, Siberian federal district of Russian Federation is given. It is shown that in Novokuznetsk value of an indicator of primary cancer incidence grew from 333,9 cases by 100 thousand population in 2005 to 372,5 cases on 100 thousand population in 2009 (totally for 18,4 %) and exceeds values of similar indicators in Russian Federation and Siberian federal district for 5 %, and indicators in the Kemerovo Region for 12 %. The ratio of the indicators in Novokuznetsk to the data in other territories is presented. It suggests that among designated territorial units the highest incidence of malignant tumors registered in Novokuznetsk. The deficit of health system capacity for inpatient care to cancer patients in the terminal stage of the disease is marked. During the 7-year period of supervision (2005-2011) the availability of hospital beds for the population of the Kemerovo Region was 11 ± 2,5 % lower than in the whole in Siberian federal district. The emphasis is placed on the fact that the deficiency of health care system limits the ability to satisfy the need for hospitalization of patients with a terminal stage of an oncological disease. The need of improvement of approaches to optimization of health system is indicated. The causes of the problems of palliative care for oncological patients are designated, the main ones are: lack of staging of rendering the palliative help, lack of integration of work of health system on ensuring the palliative help, imperfection of information support, etc. The direction for solving the existing problems is shown. Scientific and methodical base in support of its development is determined. The expected results are marked. They are the following: obtaining of reliable indicators about a condition of cancer incidence, development and implementation of improved models of palliative care in conditions of regional subordination of oncological service of Novokuznetsk, improving satisfaction of cancer patients in a terminal stage of cancer

OPTIMIZATION OF REQUIREMENT FOR MEDIAL AND RECOVERY AID TO THE PATIENTS WITH OCCUPATIONAL DISEASES AS THE WAY OF LABOUR POTENTIAL MAINTENANCE

The paper deals with the approaches to revealing the problems of communities' health, functioning public health services against social and economic reforms (at the example of the Siberian Federal District). The main basis is presented and. the basic components of determining the requirement for medical and. recovery aid. of the patients with occupational diseases included as an element into the complex target programs directed to the improvement of demographic situation, the prevention of occupational diseases and. maintenance of labor population potential are systematized

MORPHOLOGICAL CHANGES IN ORGANS AND TISSUES AT SUBCHRONIC INTOXICATION WITH BENZODIAZEPINE MEDICATIONS (EXPERIMENTAL RESEARCHES)

Morphological changes in tissues and organs at subchronic intoxication of white rats with 1,4-benzodiazepines synthetic medications (alprazolame, mezapame, diazepame, and nozepame) are studied in an experiment. Pathomorphological changes in tissues of liver, kidneys, brain and reproductive system that can lead to the development of industrially caused diseases in the employees of benzodiazepines production are revealed

THE INFLUENCE OF CONCOMITANT CARDIOVASCULAR PATHOLOGY (ISCHEMIC HEART DISEASE AND ARTERIAL HYPERTENSION) ON THE CHANGES OF RIGHT VENTRICLE OF HEART IN MINERS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

Associated cardiovascular pathology (ischemic heart disease and arterial hypertension.) in miners with chronic obstructive pulmonary disease increases the degree of hypertrophy of myocardium of right ventricle of heart and worsens its systolic and diastolic functions

Determination of the probability of the damage to the health of workers in aluminium production due to the exposure to toxic substances

In the electrolytic aluminium production fluorides in the form of hydrogen fluoride and solid fluorides, alumina-containing dust, carbon monoxide, resinous substances and benz(a)pyrene are released into the air of the working zone representing a hazard to the workers' health. The paper presents the results of the researches of the working conditions of the workers employed in the electrolytic aluminium production. The concentrations of toxic substances in the air of working areas of those engaged in anode works and electrolysis, and crane operators are given; on their basis the risks of occupational diseases (fluorosis), of acute toxic effects, of chronic intoxication, and of cancer are calculated. The average-shift concentrations of hydrogen fluoride in the air of working areas of those engaged in electrolysis are 0.40-0.46 MPC, soluble fluorine salts - 0.31-0.38 MPC, insoluble fluorine salts -0.08-0.09 MPC. In the air of the working areas of those engaged in anode works MPC excess is noted for the aerosols of mostly fibrogenic action - in 1.25 times, for resinous substances - in 1.10 times. In the air of the working areas of those engaged in electrolysis MPC excess for resinous substances is registered in 1.06 times. The average-shift concentration of insoluble fluorine salts, and carbon monoxide in the air of the working areas of those engaged in anode works and electrolysis and crane operators are within the MPC. The risk of occupational disease development (fluorosis) due to the air pollution of the working zone in the aluminium production for the workers of the major occupational groups is ranging from 0.045 to 0.0482. Hydrogen fluoride is the most hazardous contaminant in air of the working zone contributing more than 55 % to the risk of fluorosis. The risk of acute toxic effects for the workers of the major occupational groups associated with the achievement of the concentrations of the contaminants in the air of the working areas of their maximum values are ranging from 0.181 to 0.230