Wearable cassette for paper-based aptasensor designed to detection of illicit drug; ketamine- a wearable cassette prototype comparison study

The advent of 3D printing technology has spurred innovation, particularly in healthcare and biosensing. One notable application is the creation of wearable biosensors for detecting substances like ketamine, a potent anesthetic and pain reliever with medical and recreational uses. Monitoring ketamine levels is crucial due to potential misuse and health risks. Utilizing 3D printing, manufacturers can produce intricate and customizable wearable biosensors designed for ketamine detection. This flexibility permits the incorporation of various sensor types, enhancing accuracy. Traditional detection methods are often cumbersome, making 3D printing a transformative tool for real-time monitoring. The application of 3D printing in wearable biosensors has the potential to revolutionize personalized healthcare, ensuring the safe and effective usage of ketamine. In this paper 3D printed paper-based wearable aptamer cassette (3DP-PWC) has been developed by immobilizing Ketamine Aptamer on ZnO-NPs electrodes. Electrochemical techniques such as cyclic voltammetry (CV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were employed for validating results. The sensor’s versatility was demonstrated across beverages encompassing both alcoholic and non-alcoholic options. Two prototypes—a bracelet and a pendant—were developed and compared, showing promising results. Here, we reported a 3D-printing paper based wearable aptasensor for the ketamine detection. This pioneering developed sensor showed a low limit detection (LOD) of 0.01 μg/mL (lower than the physiological detection threshold 0.084 μg/mL) with linear-range was between 0.01 and 5 μmL and an optimal response time of 25 s

3D-printed cassette integrated with paper-based aptasensor for the construction of next-generation sensing tool to detect dengue virus towards plaspertronix-commercialization

The present study describes the creation of a 3D printed cassette named “3DP-PAC”, integrated to an electrochemical-aptasensor for the detection of dengue virus. It consists of an electrode cassette printed from a PLA non-conductive filament that provides a sophisticated design & support system to the delicate conductive paper-electrode. Chemically synthesized GO/ZnO-NC was used, which increases the sensor's sensitivity by accelerating the flow of electrons transferring. DENV DNA-Aptamer specifically binds to its target antigen of DENV, confirming its selectivity and demonstrating no cross reactivity with chikungunya virus antigen (CHIKV–Ag). The current study developed a 3D-based aptasensor for detecting dengue virus at a low level of detection (0.1 μg/ml). In human serum, the established platform performed well. This study paves the way for the manufacture of next-generation electrochemical biosensors utilizing 3D printing technology, with possible consequences for healthcare applications on the edge of commercialization