Unveiling the Influence of the Curve of Spee on Bite Force and Chewing Ability: A Comparative Study

Aim. To investigate the impact of the curve of Spee (CS) accentuation on bite force, chewing ability, and additionally, other factors that influence chewing ability and bite force such as restorations, caries, gender, habits, and TMJ problems. Materials and Methods. 231 participants (118 male and 113 female, mean age = ±27.96 years) were recruited for this cross-section study. Participants completed a data collection sheet in which age, gender, Angle’s classification of malocclusion, overjet, overbite, TMJ problems, habits, restorations, and caries experience were recorded. Two examiners made a lower impression, chewing ability test and measured the bite force for each participant. Measurement of the CS was obtained digitally from the poured dental cast, and the categorization was as follows: flat (2 mm). Results. The mean maximum bite force (MBF) was 468.722 N for participants with flat CS, 389.822 N for normal CS, 647.08 N for deep CS, and 384.667 N for reverse CS. The average MBF was higher for participants with deep CS (p0.05). Also, a significant difference in bite force and chewing ability was found between the three categories of CS (p<0.05). Conclusion. Bite force variations across various CS types were linked to gender and habits. Chewing ability showed no differences concerning gender, habits, TMJ problems, caries, or restorations, emphasizing CS’s significant impact on bite force while showcasing the unchanged nature of chewing ability amidst diverse factors

Comparison of Digital OPG and CBCT in Assessment of Risk Factors Associated with Inferior Nerve Injury during Mandibular Third Molar Surgery

Background: Pre-operative radiographic assessment of the anatomical relationship between the roots of the mandibular third molar and the inferior alveolar nerve (IAN) is a must to minimize the risk of IAN injury during surgery. Objectives: To compare the radiographic signs of digital orthopantomogram (OPG) and cone-beam computed tomography (CBCT). An additional objective was to assess the cortex status between the mandibular canal and third molar on CBCT images in relation to the demographic characteristics, region (right or left side), and angulation of mandibular molar. Methodology: In this retrospective study, a total of 350 impacted mandibular third molars with a close relationship between the inferior alveolar canal (IAC) and impacted mandibular third molars on digital OPG were further referred for CBCT imaging for assessment of the position of the mandibular canal. The study was conducted between August 2018 and February 2020. Digital OPGs were evaluated for radiographic signs like interruption of the mandibular canal wall, darkening of the roots, diversion of the mandibular canal, and narrowing of the mandibular canal. The age and sex of patients, site of impacted third molar, Winter’s classification of mandibular third molar, position of IAC relative to impacted molar, and the radiographic markers of OPG were assessed for cortical integrity using CBCT. Chi square testing was applied to study the values of difference and binomial logistic regression was done to assess the factors associated with cortication. Statistical significance was set at p ≤ 0.05. Results: Among 350 patients, 207 (59.1%) were male and 143 (40.9%) were female with a mean age of 36.8 years. The most common OPG sign was interruption of white line, seen in 179 (51.1%) cases. In total, 246 cases (70.3%) showed an absence of canal cortication between the mandibular canal and the impacted third molar on CBCT images. Cortication was observed in all cases with a combination of panoramic signs which was statistically significant (p = 0.047). Cortication was observed in 85 (50.6%) cases where IAC was positioned on the buccal side, 11 (16.9%) in cases of inferiorly positioned IAC, and just 8 (7.6%) for cases of lingually positioned IAC which was statistically significant (p = 0.003). Statistically insignificant (p > 0.05) results were noted for cortex status in CBCT images with regards to the age, sex, site, and angulation of impacted third molars. Conclusion: CBCT imaging is highly recommended for those cases where diversion of the mandibular canal is observed on OPG and when the roots are present between canals

Monomer Elution from Three Resin Composites at Two Different Time Interval Using High Performance Liquid Chromatography—An In-Vitro Study

Esthetics, improved colour stability and ease of contour have made photo-activated resin based restorative materials being widely used in routine dental clinical practice. Perhaps improper and inadequate polymerization of resin based composite material might lead to elution of monomer. Thus, the aim of the current study was to quantify the monomer elution from three resin composites. The intended analysis was made using high performance liquid chromatography (HPLC) at two different time periods. Three different materials that were investigated in the current study included Swiss Tech resin composite (Group A), Ceram X (Group B) and Beautifil Injectable composite (Group C). Ten cylindrical samples were fabricated in each study group. In 75% wt of ethanol, the samples were ingressed immediately and stored at room temperature. A 0.5 mL of the samples was assessed at pre-defined time intervals at 24 h and 7th day. Later, assessment of the samples was performed with HPLC and the data was analyzed using statistical test. Bisphenol A-glycidyl methacrylate (Bis-GMA), Triethylene glycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA) and Urethane dimethacrylate (UDMA) were quantified in the samples. When analyzing the release monomer, it was found that at the end of 24 h Bis-GMA was eluted more in the injectable resin composite whereas, TEGDMA was eluted from Swiss Tech and Ceram X resin composites. At the end of the 7th day it was evident that Bis-GMA was eluted maximum in all the three resin composites. Thus, monomer release was found to be evident among all three resin composites and it is of utmost important to be assessed in routine clinical practice

Nano Drug Delivery Platforms for Dental Application: Infection Control and TMJ Management—A Review

The oral cavity is an intricate environment subjected to various chemical, physical, and thermal injuries. The effectiveness of the local and systemically administered drugs is limited mainly due to their toxicities and poor oral bioavailability that leads to the limited effectiveness of the drugs in the target tissues. To address these issues, nanoparticle drug delivery systems based on metals, liposomes, polymeric particles, and core shells have been developed in recent years. Nano drug delivery systems have applications in the treatment of patients suffering from temporomandibular joint disorders such as preventing degeneration of cartilage in patients suffering from rheumatoid arthritis and osteoarthritis and alleviating the pain along with it. The antibacterial dental applications of nano-drug delivery systems such as silver and copper-based nanoparticles include these agents used to arrest dental caries, multiple steps in root canal treatment, and patients suffering from periodontitis. Nanoparticles have been used in adjunct with antifungals to treat oral fungal infections such as candida albicans in denture wearers. Acyclovir being the most commonly used antiviral has been used in combination with nanoparticles against an array of viral infections such as the herpes simplex virus. Nanoparticles based combination agents offer more favorable drug release in a controlled manner along with efficient delivery at the site of action. This review presents an updated overview of the recently developed nanoparticles delivery systems for the management of temporomandibular joint disorders along with the treatment of different oral infections

Advancements in liquid biopsy and radiology techniques for early cancer detection: A review of current methods and future directions

Aim: This review explores advancements in liquid biopsy techniques and radiological imaging for early cancer detection, highlighting their potential to improve diagnosis and treatment monitoring. Methods: We analyzed various liquid biopsy technologies, focusing on circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and exosomes, along with radiological imaging techniques such as PET scans and MRI. Key methodologies include size-based enrichment, immunoaffinity-based isolation, microfluidic technologies, and advanced imaging modalities, as well as their clinical applications and limitations. Results: Liquid biopsies offer non-invasive alternatives to traditional tissue biopsies, facilitating real-time monitoring of tumor dynamics. Techniques such as droplet digital PCR and advanced microfluidic devices have enhanced the sensitivity and specificity of biomarker detection. Radiological imaging provides complementary insights into tumor location, size, and metabolic activity, enhancing the overall diagnostic accuracy. While methods like CellSearch® for CTC counting are FDA-approved, ongoing research aims to refine isolation techniques, validate biomarkers' clinical relevance, and integrate imaging findings through large-scale trials. Conclusion: The integration of liquid biopsies and radiological imaging into routine clinical practice holds promise for personalized cancer management, enabling early detection and improved treatment responses. Continued development of these technologies is crucial for enhancing patient outcomes

Automated hematology and radiology synergy in diagnosing anemia in children

Aim: This review aims to evaluate the role of automated hematology analyzers and radiological imaging techniques in the diagnosis and management of anemia in children, highlighting traditional and advanced parameters. Methods: A comprehensive literature review was conducted, focusing on automated hematological parameters such as hemoglobin, RBC indices, reticulocyte counts, and novel metrics from modern analyzers, alongside radiological assessments such as bone marrow imaging and organ evaluation. The quasi-morphological approach, reticulocyte kinetic analysis, and imaging technologies like digital imaging and artificial intelligence were examined. Results: Traditional parameters, including RDW and MCV, provide initial insights into anemia classification. Advanced metrics, such as reticulocyte hemoglobin content and immature reticulocyte fractions, improve iron status assessment and therapeutic response evaluation. Radiological imaging offers valuable insights into bone marrow activity and organ health, complementing hematological findings. The use of automated analyzers and imaging techniques demonstrates high reproducibility and rapid results, though challenges in standardization persist. Conclusion: Automated hematology analyzers and radiological imaging techniques significantly enhance the diagnostic landscape for pediatric anemia, yet clinical integration and ongoing refinement of reference ranges are essential. Future developments in technology and standardization may further elevate the efficacy of anemia management in children