Inhibition of bovine dentin demineralization by a glutardialdehyde pretreatment:an in vitro caries study

Intact bovine dentin specimens were demineralized in 25 mmol acetic acid buffers (pH 5), with and without a 5‐min pretreatment with an acidic 2% glutardialdehyde (GDA) solution. The results demonstrate that GDA inhibits dentin demineralization and that the inhibition depends strongly on the initial calcium and phosphate concentration in the demineralization solution. The observed inhibition increases from about 5% without calcium and phosphate to 38% with a calcium and phosphate concentration initially of 6.4 mmol and 4.1 mmol, respectively. The inhibition can be explained either by a reduction of calcium and phosphate diffusion out of the lesion or by the retention of mineral inhibitors in the dentin