Qualitative and Quantitative Analysis of Sinigrin in Different Parts In Vitro and In Vivo of Brassica nigra Plants

Abstract Plants produce a wide range of organic compounds that are not directly involved in their reproduction and normal growth and development. A Laboratory and field study were conducted to optimize the concentration of Sinigrin which produced by Brassica nigra plants. Sinigrin concentrations in plant tissues at various plant parts were monitored using High Performance Liquid Chromatography (HPLC) technique. Concerning, the results of (HPLC) analysis of callus extracts showed that average amount of sinigrin were between 0.1-71.4 (µg g -1 ). Furthermore, the results of HPLC analysis inducted that sinigrin concentration in seeds, Cotyledon leaves, true leaves and stems of field grown plant were ranged between 6. 8-12.75(µg g -1 ). In conclusion, this study demonstrated that sinigrin is located in all B. nigra plant parts of different concentrations with the highest amount in the mature seeds

International Journal of Multidisciplinary and Current Research Salinity effect on wheat Triticum aestivum L. callus growth and development

Abstract This research was conducted to study the effect of salinity on callus induction and development of two local wheat cultivars Tamoz2 and Iraq. Four different methods were used to sterilize the mature seeds. Sterilizing the seeds before and after soaking them in water with absolute Ethanol for 1 min. followed by 6% NaOCl for 10 min. showed 0% contamination and didn't affect embryos viability. However, there was no significant difference in the percentage of contamination between this method and the method in which 70% Ethanol for 1 min. followed by 4.2% NaOCl for 10 min. before and after soaking them in water. Calli were induced from mature embryos and subjected to sodium chloride o