6 research outputs found
Quantification of chromosomes in live cells.
<p>A: Refractive index tomogram of a T84 cell in metaphase measured at 633 nm. B: Dry mass of the entire cell and that of the condensed chromosomes for HT-29 and T84 cells in metaphase. C: Dispersion of chromosomes in eukaryotic cells. Sample images of the refractive index map (cross-section) for a HeLa cell in metaphase measured at (i) 442 nm and (ii) 325 nm, respectively; (iii) a corresponding fluorescence image with nucleic acid stained with Syto13. D: Histogram of the refractive index for chromosomes in HT-29 cells (i, ii) and cytoplasm (iii, iv) at the wavelength of 325 nm (i, iii) and 442 nm (ii, iv), respectively. E: Dispersion parameter estimated from Fig. 3D and Eq. (4). The two distributions are statistically different (<i>p</i> = 0.0133). F: Label-free imaging of cytokinesis in a HeLa cell using RTPM. Cross-sections of the 3-D refractive index map are shown at different time points. Colorbars in A, C and F represent the refractive index.</p
Schematic layout of the regularized tomographic phase microscope (RTPM) set-up.
<p>L: lens; P: pinhole; BF: back focal plane; CL: condenser lens; S: sample; OL: objective lens; TL: tube lens; GM: galvanometer mirror; BS: beam splitter. The open circles on the right represent a trace of the focused beam in the back focal plane of condenser lens when the angle of the incident beam is varied at the sample plane.</p
Supplementary document for Optical diffraction tomography for assessing single cell models in angular light scattering - 6722127.pdf
Wolf-Mie compariso
Supplement 1: Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy
Supplementary document Originally published in Optica on 20 May 2017 (optica-4-5-546
Visualization 1: Digital micromirror device-based laser-illumination Fourier ptychographic microscopy
The reconstruction of the sample information in spatial and Fourier domain implemented by running the self-developed software written in Matlab Originally published in Optics Express on 19 October 2015 (oe-23-21-26999