Application of FT-MIR Spectroscopy for Fingerprinting Bioactive Molecules of Plant Ingredients and a New Formula with Antimicrobial Effect

Abstract. The present study aims to fingerprint 7 aromatic herbs (basil, thyme, oregano, rosemary, sage, clove, cinnamon)  and an original derived formula (EPC), in order to find specific biomarkers by Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy. The studied aromatic herbs are well known for their antioxidant, antimicrobial and anticarcinogenic effect. After comparison of each plant FT-MIR spectra results concluded that these herbs are rich in phenolic compounds like flavonols, flavanols, phenolic acids, volatile phenols. The fingerprint region was set at 1400-1760 cm-1. FTIR spectroscopy is recommended as a reliable tool to evaluate the quality and authenticity of plant formulas

Characterisation of an Aromatic Plant-based Formula using UV-Vis Spectroscopy, LC-ESI(+)QTOF-MS and HPLC-DAD Analysis

Abstract. It is known for a long time that seasoning/condimentary herbs have antioxidant activity and antibacterial properties, being good natural alternatives for disease prevention. The different efficiency of these plants is assigned to their bioactive molecules, stability and bioavailability. In the present study seven aromatic herbs (basil, thyme, oregano, rosemary, clove, cinnamon and sage) were investigated individually. A new product was developed using basil, thyme, oregano, rosemary, clove, cinnamon and sage, according to a default recipe. The characterization of each plant aimed to identify the specific “fingerprint†by its main bioactive molecules and the “traceability†of these molecules in the new product, made by mixing the selected plants according to a default recipe. In order to determine the main bioactive compounds of the individual plants composition, in comparison with the new plant-based (EPC) formula, high throughput techniques like UV-Vis spectroscopy and LC-QTOF-MS  spectrometry were used. The most important bioactive compounds determined in the studied herbs, which may exert antioxidant activity and antibacterial properties, were phenolic compounds (phenolic acids, flavonoids), quinones, clorophylls as well some polar terpenoids. The fingerprints are providing comprehensive and accurate information about the compounds that may exert antimicrobial properties. In order to assure the biological effects and the bioavailability of the polyphenols and the secondary metabolites we have to consider the antagonistic and synergistic effect that the metabolites can exert on each other