Performance of mNGS in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in non-neutropenic patients

The diagnosis of invasive pulmonary aspergillosis (IPA) diseases in non-neutropenic patients remains challenging. It is essential to develop optimal non-invasive or minimally invasive detection methods for the rapid and reliable diagnosis of IPA. Metagenomic next-generation sequencing (mNGS) in bronchoalveolar lavage fluid (BALF) can be a valuable tool for identifying the microorganism. Our study aims to evaluate the performance of mNGS in BALF in suspected IPA patients and compare it with other detection tests, including serum/BALF galactomannan antigen (GM) and traditional microbiological tests (BALF fungal culture and smear and lung biopsy histopathology). Ninety-four patients with suspicion of IPA were finally enrolled in our study. Thirty-nine patients were diagnosed with IPA, and 55 patients were non-IPA. There was significance between the IPA and non-IPA groups, such as BALF GM (P < 0.001), history of glucocorticoid use (P = 0.004), and pulmonary comorbidities (P = 0.002), as well as no significance of the other demographic data including age, sex, BMI, history of cigarette, blood GM assay, T-SPOT.TB, and NEUT#/LYMPH#. The sensitivity of the BALF mNGS was 92.31%, which was higher than that of the traditional tests or the GM assays. The specificity of BALF mNGS was 92.73%, which was relatively similar to that of the traditional tests. The AUC of BALF mNGS was 0.925, which presented an excellent performance compared with other traditional tests or GM assays. Our study demonstrated the important role of BALF detection by the mNGS platform for pathogen identification in IPA patients with non-neutropenic states, which may provide an optimal way to diagnose suspected IPA disease

25 Gb/s Data Transmission Using a Directly Modulated InGaAlAs DBR Laser over 14 nm Wavelength Tuning Range

With the deployment of the fifth generation of mobile networks (5G), 25 and 100 Gb/s directly modulated lasers and modules will become the mainstream optical transmitters. A directly modulated InGaAlAs/InP distributed Bragg reflector (DBR) laser is fabricated by butt-joint technology. A 25 Gb/s data transmission over a single-mode fiber of up to 10 km is demonstrated, and a wavelength tuning range of 14.28 nm is achieved through injection current tuning of a DBR section and temperature control of a thermoelectric cooler (TEC), which is the best candidate of colorless light sources for wavelength-division-multiplexed passive optical network (WDM-PON) systems

High Optical Feedback Tolerance of a Detuned DBR Laser for 10-Gbps Isolator-Free Operation

The optical feedback tolerance (OFT) of a distributed Bragg reflector (DBR) laser was investigated experimentally. The static and modulation performance of the DBR laser under optical feedback was examined by evaluating its relative intensity noise (RIN) and bit error rate (BER). It is shown that the OFT of the DBR laser is closely related to its peak wavelength detuning relative to the Bragg wavelength. A high tolerance to optical feedback was demonstrated, both in the continuous wave (CW) and the direct modulation (DM) states, when the DBR laser was red-detuned relative to the Bragg wavelength. The excess RIN induced by optical feedback was well suppressed to a level of −140 dB/Hz at a feedback level of −9 dB in the CW state. In a 10 Gbps direct modulation state, bit error rates (BER) below 1 × 10−9 and 3 × 10−7 were obtained under a feedback level of −15 dB and −9 dB, respectively, for the case of back-to-back transmission. After 20 km fiber transmission, the BER still maintained below 1 × 10−7 under a feedback level of −15 dB, with a power penalty of less than 1 dB

Data on the association of CMPK1 with clinicopathological features and biological effect in human epithelial ovarian cancer

Human epithelial ovarian cancer (EOC) is the most lethal gynecological disease. However, the molecular mechanisms by which transforming growth factor-β (TGF-β) regulates ovarian tumor progression markers remain unclear. The present data show cytidine monophosphate kinase (CMPK) as an EOC biomarker and are related to the article entitled “Cytidine monophosphate kinase is inhibited by the TGF-β signalling pathway through the upregulation of miR-130b-3p in human epithelial ovarian cancer” [1]. CMPK, as well as cystatin B [2] and β-2-microglobulin [3], is overexpressed in human epithelial-type ovarian tumors. CMPK is an enzyme required for nucleic acid biosynthesis [4] and is regulated by the TGF-β signaling pathway in EOC cells [1]. Furthermore, the data show the effect of CMPK-shRNA on EOC cell apoptosis and TGF-β-induced Smad2 phosphorylation. CMPK expression in two EOC cell lines OVCAR-3 and SK-OV-3 is regulated by multiple miRNAs and some of these miRNAs may affect EOC chemoresistance [5]. Keywords: TGF-β signaling, UMP/CMP kinase, Tissue microarray, Tumorigenesis, miRNA, Therapeutic targe