Preventive and Curative Effects of Atriplex Halimus L. Aqueous Extract on Benzene Provoked Hepatic Injury in Rats

Objective: The present study was designed to estimate the preventive and curative potency of aqueous extract of Atriplex halimus L. (Ah) aerial parts against benzene (BZ) intoxication in liver rats. Methods: 30 male albino rats were divided into five groups of 6 rats each: Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in drinking water for 15 weeks. Aqueous extract of Atriplex halimus was received intragastrically during the last 30 days of BZ exposure for curative treatment (AhC) and all the duration of BZ exposure for preventive treatment (AhP). At the end the experiment, body weight gain and relative liver weight were estimated and liver enzyme markers (AST, ALT, ALP and LDH) were analysed by spectrophotometry. Histopathological studies on hepatic tissue were also performed by the method of Hematoxylin and Eosin staining. Results: Benzene administrated to rats caused an alteration in physiological parameters (body and organs weigh) and hepatic enzymes markers (AST, ALT, ALP and LDH). In addition, Histopathological studies showed a massive degeneration in liver tissue in BZ-exposed rats. However, treatment by Atriplex halimus especially preventive effect ameliorated most of the adverse effects induced by benzene. Ah restored the altered of physiological, biochemical and histopathological changes. Conclusion: The present study suggests that Atriplex halimus extract could be a substantially promising hepatoprotective agent against benzene toxic effects and may be against other hepatotoxic chemical or drugs. Keywords: Atriplex halimus, Benzene, hepatotoxicity, Rats

PROTECTIVE ROLE OF AQUEOUS EXTRACT OF ATRIPLEX HALIMUS L. AGAINST BENZENE-INDUCED DAMAGE ON RENAL FUNCTION AND GLOMERULAR CELLS IN RATS

Objective: Benzene (BZ) is an important pollutant compound, present in both occupational and general environment that has been linked to adverse health effect. The aim of the current study was to investigate the curative and preventive effect of Atriplex halimus L. (Ah) extract against BZ-induced nephrotoxicity in rats. Methods: A total of 30 male albino Wistar rats were divided into five groups (n=6): Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in their drinking water for 15 weeks. Ah (Aqueous Extract of aerial parts) was given by gavage during the past 30 days of BZ-exposed for curative treatment (AhC) and all the duration of BZ exposure in the animals for preventive treatment (AhP). Some biochemical, oxidative stress parameters, and histopathology of kidney tissue were studied. Results: Obtained results revealed that BZ exposure to rats caused a significant elevation in urea, creatinine, and malondialdehyde levels. Then, it led to reducing the glutathione level, catalase, and glutathione-S-transferase activities when compared to the control animals. Histopathological studies showed a massive damage in kidneys of the BZ-exposed rat. Our results showed that curative treatment of aqueous extract of Ah has a partial correction of the biochemical, oxidative stress parameters, and kidney morphology, but preventive treatment has a dramatic effect on the previous parameters. Conclusion: Results demonstrated the beneficial effects of Ah preventive treatment against stress oxidative and kidney damage induced by BZ

Protective effects of Aristolochia longa and Aquilaria malaccensis against lead induced acute liver injury in rats

Objective: To investigate the protective effects of Aristolochia longa (A. longa) and Aquilaria malaccensis (A. malaccensis) on acute hepatotoxicity induced by lead in female albino rats.Methods: Twenty five (25) apparently healthy female Wistar rats were randomly divided into five groups of five rats in each: control, Pb, Pb + A. longa (Ar), Pb+ A. malaccensis (Aq), and Pb+ A. longa (Ar) + A. malaccensis (Aq) lead (100 mg/kg b.w.) as Pb (C2H3O2)2 added in their drinking water for 75 days. A. longa (rhizome powder at a dose of 10 g/kg of diet) and A. malaccensis (heartwood powder at a dose 10 g/kg of diet) were added to the feed during the last 15 days of lead exposed in the animals.Results: Obtained results revealed that lead treatment caused a significant increase in serum GOT, GPT and ALP activities and in liver of MDA level and CAT activity. In contrast, it led to an decrease in the liver GOT, GPT and GST activities and in GSH level in rats. Also, the results clearly showed that lead causes alterations of hepatic tissue in comparison with controls. Our results showed that treatment with A. malaccensis and A. longa a partial correction of the previous parameters. The histological observations confirmed the hepatoprotection results by the biochemical parameters.Conclusions: Results demonstrated beneficial effects of A. longa and A. malaccensis treatment in Pb-induced oxidative stress and tissue damage in liver

Protective effects of Aristolochia longa and Aquilaria malaccensis against lead induced acute liver injury in rats

Objective: To investigate the protective effects of Aristolochia longa (A. longa) and Aquilaria malaccensis (A. malaccensis) on acute hepatotoxicity induced by lead in female albino rats.Methods: Twenty five (25) apparently healthy female Wistar rats were randomly divided into five groups of five rats in each: control, Pb, Pb + A. longa (Ar), Pb+ A. malaccensis (Aq), and Pb+ A. longa (Ar) + A. malaccensis (Aq) lead (100 mg/kg b.w.) as Pb (C2H3O2)2 added in their drinking water for 75 days. A. longa (rhizome powder at a dose of 10 g/kg of diet) and A. malaccensis (heartwood powder at a dose 10 g/kg of diet) were added to the feed during the last 15 days of lead exposed in the animals.Results: Obtained results revealed that lead treatment caused a significant increase in serum GOT, GPT and ALP activities and in liver of MDA level and CAT activity. In contrast, it led to an decrease in the liver GOT, GPT and GST activities and in GSH level in rats. Also, the results clearly showed that lead causes alterations of hepatic tissue in comparison with controls. Our results showed that treatment with A. malaccensis and A. longa a partial correction of the previous parameters. The histological observations confirmed the hepatoprotection results by the biochemical parameters.Conclusions: Results demonstrated beneficial effects of A. longa and A. malaccensis treatment in Pb-induced oxidative stress and tissue damage in liver