20 research outputs found
Characterization and quantitative topographical distribution of salmon calcitonin-binding sites in rat kidney sections
Get PDFAbstractRenal binding sites for labelled salmon calcitonin (sCT) were studied using cryostat sections and autoradiography. Increasing concentrations of unlabelled sCT inhibited 125I-sCT binding. 125I-sCT bound to a single site with a Kd of 2 nM and a number of sites of 220 fmolmg protein. Mammalian calcitonins had low affinities and peptides unrelated to CT were devoid of any significant affinity for 125I-sCT receptors. Auto-radiograms disclosed a high concentration of 125I-sCT receptors mainly located in the outer medulla and heterogeneously in the renal cortex. The distribution of specific binding sites is in agreement with the current concepts of renal action of calcitonin
Down-regulation of rat kidney calcitonin receptors by salmon calcitonin infusion evidenced by autoradiography.
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Insulin receptors along the rat nephron: [125I] Insulin binding in microdissected glomeruli and tubules
No full textDifferential short-term desensitization to vasopressin, isoproterenol, glucagon, parathyroid hormone and calcitonin in the thick ascending limb of rat kidney
No full textHuman parathyroid hormone-related protein and human parathyroid hormone receptor type 1 are expressed in human medulloblastomas and regulate cell proliferation and apoptosis in medulloblastoma-derived cell lines
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