EFFECT Of CALCIUM CONCENTRATION IN NUTRIENT SOLUTION AND ITS APPLICATION TIME ON TOMATO FRUIT COMPOSITION .

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Analysis of Geometric Relation between Cerebral Contusion and Site of Impact in Traumatic Brain Injury Patients

Cerebral contusion is found in 17–34% of traumatic brain injury (TBI) cases. The inertial and contact effects responsible for this injury makes the presence and location of contusion almost impossible to detect without appropriate imaging techniques. This study aimed to identify the geometric association between site of impact and cerebral contusion in patients with TBI. Data were collected retrospectively from Dr. Hasan Sadikin Hospital Bandung, Indonesia from January 2016–December 2017. TBI patients were scanned for diagnosis of cerebral contusion on the initial CT scan and the presence of soft tissue swelling as the site of impact. The locations were then geometrically classified into four groups; frontal, right temporoparietal, left temporoparietal, and occipital. Analysis was then performed using the multinomial logistic regression method with α=0.05 and 95% confidence interval (CI). Right temporoparietal contusion was associated with contrecoup impact compared to frontal impact (p=0.008, OR 6.8, 95% CI: 1.6–28.7) and occipital impact (p=0.002, OR 6.8, 95% CI: 1.7–26.8) while left temporoparietal contusion was associated with contrecoup impact compared to frontal (p=0.006, OR 6.8, 95% CI: 1.7–26.8) and occipital impact (p=0.016, OR 10, 95% CI: 1.3-17) and with coup impacts compared to frontal impacts (p=0.025, OR 5.3, 95% CI: 1.2–23.3). It is concluded that temporal contusion has a statistically significant correlation with contrecoup impacts. Hubungan Analisis Geometris antara Kontusi Serebral dan Lokasi Benturan pada Pasien Cedera Otak TraumatisKontusi serebral ditemukan pada 17–34% kasus cedera otak traumatika (TBI). Efek inersia dan kontak yang bertanggung jawab untuk cedera ini membuat keberadaan dan lokasi benturan hampir mustahil untuk dideteksi tanpa teknik pencitraan yang tepat. Penelitian ini bertujuan untuk menemukan hubungan geometris antara kontusi serebral dan lokasi benturan yang terjadi pada pasien TBI. Data dikumpulkan secara retrospektif dari Rumah Sakit Dr. Hasan Sadikin Bandung, dari Januari 2016–Desember 2017. Pasien TBI dipindai untuk diagnosis kontusio serebral menggunakan CT scan awal dan adanya pembengkakan jaringan lunak sebagai tempat terjadinya benturan. Lokasi itu kemudian secara geometris diklasifikasikan menjadi empat kelompok; frontal, temporoparietal kanan, temporoparietal kiri, dan oksipital, kemudian dianalisis menggunakan metode regresi logistik multinomial dengan α=0,05 dan interval kepercayaan (IK) 95%. Kontusio temporoparietal kanan dikaitkan dengan impact contrecoup dibanding dengan impact frontal (p=0,008, OR 6,8, IK 95% 1,6–28,7) dan impact oksipital (p=0,002, OR 6,8, IK 95% 1,7–26,8), sementara kontusi temporoparietal kiri dikaitkan dengan impact contrecoup dibandingkan dengan impact frontal (p=0,006, OR 6,8, IK 95% 1,7–26,8) dan impact oksipital (p=0,016, OR 10, IK 95% 1,3–17) dan dengan impact coup dibanding dengan impact frontal (p=0,025, OR 5,3, IK 95% 1.2–23.3). Simpulan, bahwa kontusio temporal memiliki korelasi yang signifikan secara statistik dengan impact contrecoup

Architecture in Sudan: The Post–Independence Era (1956-1970). Focus on the Work of Abdel Moneim Mustafa

This article is part of a study on the Sudanese social and political context during the formation of the Modern Movement and the manifestations in built form and spatial expression during the period 1900-1970. The study has been on–going for several years and includes a literature search, local surveys (of unpublished and undocumented information) as well as photographs taken by the authors, sourced from architects or published material. It is argued that the Sudanese response to the International Style was in fact early experimentation in critical regionalism. The most notable architectural heritage in Sudan are the archaeological remains at Kerma and Napata as well as the remains of ancient Meroe about 180 km north of Khartoum. These cultures demonstrated sophistication in building materials and construction techniques. Due to climate changes, political changes and religious changes over a large stretch of time (642AD with the signing of the Bagt Treaty–1898 at the demise of the Mahdist era) the qualities of the built environment became more transient and rudimentary in character with a greater focus on manifesting tradition through body images, clothing and rituals that were not necessarily tied to a particular physical location rather than through monuments. With foreign interest in the strategic location of the Sudan, and as a part of the scramble for Africa, came specific stylistic and technical manifestations

The Plasmodium alveolin IMC1a is stabilised by its terminal cysteine motifs and facilitates sporozoite morphogenesis and infectivity in a dose-dependent manner.

Apicomplexan parasites possess a unique cortical cytoskeleton structure composed of intermediate filaments. Its building blocks are provided by a conserved family of proteins named alveolins. The core alveolin structure is made up of tandem repeat sequences, thought to be responsible for the filamentous properties of these proteins. A subset of alveolins also possess conserved motifs composed of three closely spaced cysteine residues situated near the ends of the polypeptides. The roles of these cysteine motifs and their contribution to alveolin function remains poorly understood. The sporozoite-expressed IMC1a is unique within the Plasmodium alveolin family in having conserved cysteine motifs at both termini. Using transgenic Plasmodium berghei parasites, we show in this structure-function analysis that mutagenesis of the amino- or carboxy-terminal cysteine motif causes marked reductions in IMC1a protein levels in the parasite, which are accompanied by partial losses of sporozoite shape and infectivity. Our findings give new insight into alveolin function, identifying a dose-dependent effect of alveolin depletion on sporozoite size and infectivity, and vital roles of the terminal cysteine motifs in maintaining alveolin stability in the parasite

O2-independent demethylation of trimethylamine N-oxide by Tdm of Methylocella silvestris

Bacterial trimethylamine N-oxide (TMAO) demethylase, Tdm, carries out an unusual oxygen-independent demethylation reaction, resulting in the formation of dimethylamine and formaldehyde. In this study, sitedirected mutagenesis, homology modelling and metal analyses by inorganic mass spectrometry have been applied to gain insight into metal stoichiometry and underlying catalytic mechanism of Tdm of Methylocella silvestris BL2. Herein, we demonstrate that active Tdm has 1 molar equivalent of Zn2+ and 1 molar equivalent of non-heme Fe2+. We further investigated Zn2+ and Fe2+-binding sites through homology modelling and sitedirected mutagenesis and found that Zn2+ is coordinated by a 3-sulfur-1-O motif. An aspartate residue (D198) likely bridges Fe2+ and Zn2+ centres, either directly or indirectly via H-bonding through a neighbouring H2O molecule. H276 contributes to Fe2+ binding, mutation of which results in an inactive enzyme, and the loss of iron, but not zinc. Site-directed mutagenesis of Tdm also led to the identification of three hydrophobic aromatic residues likely involved in substrate coordination (F259, Y305, W321), potentially through a cation- interaction. Furthermore, a cross-over experiment using a substrate analogue gave direct evidence that a trimethylamine-alike intermediate was produced during the Tdm catalytic cycle, suggesting TMAO has a dual role of being both a substrate and an oxygen donor for formaldehyde formation. Together, our results provide novel insight into the role of Zn2+ and Fe2+ in the catalysis of TMAO demethylation by this unique oxygenindependent enzyme

Removal of Carcinogenic Hexavalent Chromium by Reduction with Iron in Presence of Accelerator Non Toxic Organic Compounds (Chalcone)

The focus of this work is on Cr, which has two predominant oxidation states of the atmosphere: + â€3, which is an essential nutrient in low doses, and +â€6, which is highly toxic and carcinogenic. We found that, the reduction of Cr6+ to Cr3+ was first order reaction and the rate controlling step is the diffusion of (Cr6+) from bulk to iron surface. Toxicity reduction method includes studding of 1-aryl-3-(1-phenylpyrazolo [3,4-b] quinoxalin-3-yl) -2-propenones derivatives or chalcone derivatives concentration, temperatures and rotation speeds effect, which exhibit a strong dependence and supported that the process is diffusion controlled. Thermodynamic parameter ΔG*, ΔH* and ΔS* are given. Mass transfer study of the process was correlated by the equation: Sh = 0.53Sc0.33Re0.38. The order of acceleration was confirmed by quantum calculations

Care Bundle Application Decreases The Frequency and Severity of Intraventricular Hemorrhage in Preterm Neonates: Single Center Study

Background: Intraventricular hemorrhage (IVH) is a severe complication in preterm babies admitted to Neonatal Intensive Care Units (NICU). Advanced stages of IVH predispose to neurological deficits such as cerebral palsy and hydrocephalus. There are numerous strategies and policies implemented in NICUs around the world to decrease the incidence of IVH in preterm babies and prevent its ensuing neurodevelopmental complications. Aim of the Work: To study the effect of implementing a bundle of care on incidence and severity of IVH among preterm neonates. Materials and Methods: Retrospective analysis of patient records for incidence and severity of IVH between May and August 2018 was done. This was followed by an educational interim period where NICU staff received training of pre-natal, natal, and postnatal care bundle guidelines to reduce IVH. The guidelines were then implemented on all preterm babies (28-34 weeks gestational age (GA)) born and admitted to Kasr Al Ainy Teaching Hospital NICU, Cairo University between October 2018 and January 2019 (n=58). Cranial ultrasonography was performed at week 1 and week 2-3 of life. Frequency and severity of IVH were compared among studied groups. Risk factors for IVH were analyzed and recorded. Results: The frequency of IVH was significantly lower in the post-bundle group (44-46% pre-bundle, and 27% post-bundle) especially evidenced by the ultrasonography in week two of life. Severity of IVH also improved post bundle since there were no grade III IVH patients in that group. Exposure to hypocapnia, blood pressure fluctuations, IV fluid boluses and administration of sodium bicarbonate were significantly correlated with development of IVH in preterm babies (p = 0.001). Conclusion: Implementation of an IVH care bundle that includes perinatal measures can positively affect the neurological outcome by decreasing incidence and severity of IVH in preterm babies

Nurses experience of caring for patients with COVID-19 : a phenomenological study

Introduction: COVID-19 has impacted all dimensions of life and imposed serious threat on humankind. Background: In Jordan, understanding how nurses experienced providing care for patients with COVID-19 offers a framework of knowledge about similar situations within the context of Arabic culture. Aim: To explore nurses' experience with providing hands-on care to patients with active COVID-19 infection in an Arabic society. Methods: A descriptive phenomenological study interviewed 10 nurses through a purposive sampling approach until data saturation was reached. The research site was hospital designated to receive patients with active COVID-19 infection. Semi-structured interviews were used to collect the data. Findings: Three themes were generated from the data: the impact of the COVID-19 outbreak on nurses' health; unfamiliar work and social environments; and conforming to professional standards. Discussion: There are specific risks to the physical and mental wellbeing of nurses who provide hands-on care to patients with COVID-19 in an Arabic society. Implication for nursing and health policy: Health care institutions should consider establishing programs that promote nurses' wellbeing and support their productivity in a crisis. A danger pay allowance should be considered for nurses during extraordinary circumstances, such as pandemics