Rheological behaviour of dextran sulfate solutions

The purpose of this research was to study the rheological behaviour of dextran sulfate solutions under different conditions. A PC controlled rotational viscometer with a cone and plate geometry equipped with a temperature controlled unit was used to measure apparent viscosity of dextran solutions at different temperatures (25, 40 and 60 oC) and concentrations (2, 5, 10 and 20%). Viscosity tests of dextran sulfate solutions were performed at a range of shear rates (8-230 1/s) and the rheological data were fitted using the Power Law equation to get consistency and flow behaviour indices. The results indicated that all dextran sulfate solutions showed Newtonian flow behaviour. The viscosity of dextran sulfate solutions was significantly affected by temperature, e.g. the viscosity of a 5% solution at 25, 40 and 60 oC was 16.7, 12.7 and 10.7 mPa.s, respectively

Binding studies of crocin to β-Lactoglobulin and its impacts on both components

© 2020 Elsevier Ltd Light susceptibility of crocin and high degree of unsaturation limit its application in food systems. This study aimed to investigate the impact of molecular binding of crocin to β-lactoglobulin (β-LG) on crocin stability as well as the conformational stability of β-LG. The overall binding affinity was estimated about 1.74 × 103 M−1 through following UV spectral changes of β-LG at different concentrations of crocin. A 1:1 binding stoichiometry for the complex of crocin and β-LG was recognized using Job's method. The results of molecular docking and molecular dynamic simulation indicated that in addition to a single hydrogen bond with an oxygen linker attached to polyene chain, hydrophobic interactions play the major role in binding of crocin's polyene chain to β-LG surface. Three-dimensional emission and CD spectra showed conformational alterations in both tertiary and secondary structure of β-LG varying with crocin concentration. Depending on the temperature, crocin showed a dual effect on the thermodynamic stability of β-LG. Crocin destabilized β-LG up to room temperature, but stabilized at higher temperatures. The CD spectra of crocin in the Soret region and simulation results proved the bending of polyene chain and changes in the angle between sugar groups and polyene chain in combination with the protein. β-LG complexation enhanced the chemical stability of crocin by 20% during a 30-day storage, while its degradation was accelerated under UV irradiation. According to the results, it is predicted crocin functionalities to be affected by β-LG association and vice versa