43 research outputs found
The technique of inhalation anaethesia in experimental investigation in the rat
The rat is the most frequently used animal in scientific inquiry conducted for the
purpose of advancing basic knowledge that may lead to an improvement in the
results of treatment. Understanding of the pharmacological properties of inhalation
anaesthetics, in combination with monitoring of their concentration in
the inspired and end-tidal gas, together provide safe and precise control of the
depth of the anaesthesia. However, accurate application of the inhalation method
of anaesthesia requires special equipment for the delivery and effective scavenging
of inhalation anaesthetics
The pathophysiology of intracerebral haemorrhage
Spontaneous intracerebral haemorrhage carries a high mortality rate and treatment
of the disease raises more questions then answers. Mass effect, ischaemia and toxicity
of blood components are responsible for brain tissue damage. Initially occurring
disturbances of cerebral blood flow have a temporary character and do not
play a key role in the pathology of intracerebral haematoma. Oedema formatting in
the 24–48 hours after intracerebral bleeding is the result of multidirectional processes.
The pathological mechanism that underlines it is the function of activation of
systemic complement and cascade of coagulation. In the light of these findings,
further clinical and experimental investigations should be focused on these factors
Apoptosis in the course of experimetal intracerebral haemorrhage in the rat
Intracerebral haematoma was produced in 25 adult rats by infusion of 100 µl of
autologous blood into the striatum. The animals’ brains were removed at 1, 3,
7, 14 and 21 days after production of the haematoma. The TUNEL method was
used to detect DNA fragmentation and TUNEL-positive cells were qualified.
TUNEL-positive cells were already found on the first day of observation and
were present for three weeks after haematoma production.
These results provide evidence that programmed cell death is associated with
intracerebral haemorrhage