ASSESSMENT OF ANTIMICROBIAL RESISTANCE OF Salmonella spp. ISOLATED FROM FARMED ASIAN CLAM Corbicula fluminea

U ovom istraživanju, Salmonella spp. je uspješno izolirana iz azijskog školjkaša Corbicula fluminea primjenom Ksiloza Lizin Deoksiolata (XLD) selektivnog agara. Ukupno je izolirano 200 bakterijskih kolonija iz živih azijskih školjkaša i podvrgnuto ispitivanju osjetljivosti na antimikrobne tvari primjenom disk difuzije. U istraživanju je primijenjeno ukupno 18 antibiotika, i to: oksolinska kiselina, nalidiksična kiselina, eritromicin, tetraciklin, doksiciklin, oleandomicin, oksitetraciklin, spiramicin, ampicillin, kanamicin, fosfomicin, florfenikol, linkomicin, novobiocin, kloramfenikol, amoksicilin, flumekvin i sulfametoksazol. Rezultati ove studije ukazali su da je ukupni uzorak osjetljivih na antibiotike za Salmonella spp. izolirane iz uzorka C. fluminea bilo 50% ili 1800 slučajeva. Potom su uslijedili slučajevi rezistencije na antibiotike od 45% ili 1620 slučajeva i posredno osjetljivih na antibiotike (5% ili 180 slučajeva). Na temelju rezultata, tetraciklin, doksiciklin, oksitetraciklin i flumekvin pokazali su najveću inhibiciju izolirane Salmonella spp. Indeks višestruke rezistencije na antibiotike (MAR) bio je 0,36, što ukazuje da su uzorci školjkaša bili visoko izloženi testiranim antibioticima.In the present study, Salmonella spp. was successfully isolated from Asian clam Corbicula fluminea by using Xylose Lysine Deoxycholate (XLD) selective agar. A total of 200 bacterial colonies from live Asian clams were isolated and subjected to antimicrobial sensitivity test by using disc diffusion method. A total of 18 antibiotics was applied in the present study, namely oxolinic acid, nalidixic acid, erythromycin, tetracycline, doxycycline, oleandomycin, oxytetracycline, spiramycin, ampicillin, kanamycin, fosfomycin, florfenicol, lincomycin, novobiocin, chloramphenicol, amoxycillin, flumequine and sulphamethoxazole. The findings of the present study showed that total antibiotic sensitive case for Salmonella spp. isolated from C. fluminea sample was 50% or 1800 cases. This was followed by antibiotic resistance case 45% or 1620 cases and intermediary antibiotic sensitive case (5% or 180 cases). Based on the results of the present study, tetracycline, doxycycline, oxytetracycline and flumequine showed the highest inhibition of isolated Salmonella spp. The multiple antibiotic resistance (MAR) index was 0.36, indicating the sampled clams were highly exposed to the tested antibiotics

Assessment of Antimicrobial Resistance of Salmonella spp. Isolated from Farmed Asian Clam Corbicula Fluminea

In the present study, Salmonella spp. was successfully isolated from Asian clam Corbicula fluminea by using Xylose Lysine Deoxycholate (XLD) selective agar. A total of 200 bacterial colonies from live Asian clams were isolated and subjected to antimicrobial sensitivity test by using disc diffusion method. A total of 18 antibiotics was applied in the present study, namely oxolinic acid, nalidixic acid, erythromycin, tetracycline, doxycycline, oleandomycin, oxytetracycline, spiramycin, ampicillin, kanamycin, fosfomycin, florfenicol, lincomycin, novobiocin, chloramphenicol, amoxycillin, flumequine and sulphamethoxazole. The findings of the present study showed that total antibiotic sensitive case for Salmonella spp. isolated from C. fluminea sample was 50% or 1800 cases. This was followed by antibiotic resistance case 45% or 1620 cases and intermediary antibiotic sensitive case (5% or 180 cases). Based on the results of the present study, tetracycline, doxycycline, oxytetracycline and flumequine showed the highest inhibition of isolated Salmonella spp. The multiple antibiotic resistance (MAR) index was 0.36, indicating the sampled clams were highly exposed to the tested antibiotics