Antibiotic resistance in the pathogenic bacteria isolated from environmental samples of the food production facilities

Foodborne pathogens, such as Salmonella spp. and Listeria monocytogenes, may be present at various stages of production. The overuse of antibiotics over the past few decades has led to the emergence of many antibiotic-resistant bacteria, including foodborne pathogens. We investigated the sensitivity of Salmonella spp and Listeria monocytogenes to various antibiotics. Studied strains of L. monocytogenes and Salmonella spp. they showed resistance to tetracycline, which totaled 66.7%. High resistance of strains to ampicillin was also noted (57.14%). All strains were sensitive to amikacin, azithromycin, Meropenem, ciprofloxacin, moxifloxacin, norfloxacin. All strains of Salmonella spp. were resistant to ampicillin and penicillin G. Resistance to streptomycin and trimethoprim/sulfamethoxazole was 91.7%. All strains of L. monocytogenes were resistant to nalidixic acid. 22.2% of the strains showed resistance to tetracycline and cefepime. 11.1% of the strains were resistant to chloramphenicol and trimethoprim/sulfamethoxazole

Antibiotic resistance in the pathogenic bacteria isolated from environmental samples of the food production facilities

Foodborne pathogens, such as Salmonella spp. and Listeria monocytogenes, may be present at various stages of production. The overuse of antibiotics over the past few decades has led to the emergence of many antibiotic-resistant bacteria, including foodborne pathogens. We investigated the sensitivity of Salmonella spp and Listeria monocytogenes to various antibiotics. Studied strains of L. monocytogenes and Salmonella spp. they showed resistance to tetracycline, which totaled 66.7%. High resistance of strains to ampicillin was also noted (57.14%). All strains were sensitive to amikacin, azithromycin, Meropenem, ciprofloxacin, moxifloxacin, norfloxacin. All strains of Salmonella spp. were resistant to ampicillin and penicillin G. Resistance to streptomycin and trimethoprim/sulfamethoxazole was 91.7%. All strains of L. monocytogenes were resistant to nalidixic acid. 22.2% of the strains showed resistance to tetracycline and cefepime. 11.1% of the strains were resistant to chloramphenicol and trimethoprim/sulfamethoxazole

The role of monitoring production environment facilities to support microbiological safety and food quality in meat processing plants

The results of many studies prove the microbiota of the surfaces of the production environment can be a source of food contamination. Environmental monitoring allows to identify problem areas in the enterprise and take corrective actions to eliminate them. This work is conducted to the analysis of the microbiota of abiotic objects selected in the area of close proximity to food products at a pork processing plant by sequencing the 16S RNA gene. The phylum Proteobacteria (from 37.7 to 73.6%), Firmicutes (from 0.14 to 18.6%), Bacteroidota and Actinobacteriota were the dominant components of the microbial communities of the meat processing enterprise. Bacteria of the genus Pseudomonas were found in all samples, the number of readings of these bacteria ranged from 1.90% to 28.76% of the total number of readings. Bacteria of the genus Brochotrix were found in samples from 0.02% to 2.75%. The identification of this phylum indicates the potential presence of pathogenic microorganisms and spoilage microorganisms at production facilities, which can negatively affect the quality and safety of food products

Preparation of Biocidal Nanocomposites in X-ray Irradiated Interpolyelectolyte Complexes of Polyacrylic Acid and Polyethylenimine with Ag-Ions

Due to the presence of cationic units interpolyelectrolyte complexes (IPECs) can be used as a universal basis for preparation of biocidal coatings on different surfaces. Metallopolymer nanocomposites were successfully synthesized in irradiated solutions of polyacrylic acid (PAA) and polyethylenimine (PEI), and dispersions of non-stoichiometric IPECs of PAA–PEI containing silver ions. The data from turbidimetric titration and dynamic light scattering showed that pH 6 is the optimal value for obtaining IPECs. Metal polymer complexes based on IPEC with a PAA/PEI ratio equal to 3/1 and 1/3 were selected for synthesis of nanocomposites due to their aggregative stability. Studies using methods of UV–VIS spectroscopy and TEM have demonstrated that the size and spatial organization of silver nanoparticles depend on the composition of polymer systems. The average sizes of nanoparticles are 5 nm and 20 nm for complexes with a molar ratio of PAA/PEI units equal to 3/1 and 1/3, respectively. The synthesized nanocomposites were applied to the glass surface and exhibited high antibacterial activity against both gram-positive (Staphylococcus aureus) and gram-negative bacteria (Salmonella). It is shown that IPEC-Ag coatings demonstrate significantly more pronounced biocidal activity not only in comparison with macromolecular complexes of PAA–PEI, but also coatings of PEI and PEI based nanocomposites