Análise morfológica e imunológica das placentas de ratas com diabete de intensidade moderada

Avaliar os efeitos do diabete moderado nos parâmetros reprodutivos maternos e no desenvolvimento placentário-fetal em ratas Wistar. Metodologia: No dia do nascimento, 147 ratas Wistar foram distribuídas aleatoriamente em dois grupos experimentais: Grupo não-diabético (Controle, n=45) - recebeu o veículo; Grupo diabético (STZ, n=102) - recebeu 100 mg streptozotocin/kg. Na fase adulta, as ratas foram acasaladas e, no dia 0 de prenhez, foram incluídas ratas controle que apresentassem glicemia abaixo de 120 mg/dL e, para o grupo diabete moderado, glicemia entre 120 e 300 mg/dL. Em diferentes momentos da prenhez, glicemia e peso corpóreo foram verificados. No 21º dia de prenhez, as ratas foram anestesiadas para coleta de sangue para dosagem de insulina e, em seguida, foi realizada laparotomia para retirada e pesagem dos fetos e placentas. Os dados maternos e fetais foram analisados por Twoway ANOVA seguida do Teste t. Os recém-nascidos (RN) foram classificados em pequenos, adequados e grandes para idade de prenhez e as comparações entre os grupos foram realizadas segundo o Teste de Qui-quadrado. As ratas STZ apresentaram glicemias maiores nos dias 0 e 14 de prenhez, menor número médio de fetos vivos, implantações e de corpos lúteos, aumento nas taxas de perdas embrionárias pós-implantação, no peso placentário e na proporção de RN pequenos (PIP) e grandes (GIP) para idade de prenhez, redução de RN AIP e inalteração nas concentrações de insulina. Portanto, o diabete de intensidade moderada alterou a glicemia materna no início da prenhez, que deflagrou alterações no organismo materno e/ou no desenvolvimento inicial do embrião, afetando sua implantação e futuro desenvolvimento placentário e fetal.To evaluate the mild diabetes effects on the maternal reproductive outcome and placental-fetal development in female Wistar rats. Methodology: At the birth day, 147 female rats were randomly distributed in two experimental groups: 1) Non-diabetic Group (Control, n=45) - received the vehicle; 2) Diabetic Group (STZ, n=102) - received 100 mg streptozotocin/kg. At the adult phase, the female rats were mated and, at the day 0 of pregnancy, they were included in the control group when presented glycemia below 120 mg/dL and, in the group STZ when showed glycemia between 120 and 300 mg/dL. In different moments of the pregnancy, glycemia and body weight were verified. At day 21 of pregnancy, the rats were anaesthetized to collect blood samples for insulin determination and, soon afterwards, the laparotomy was carried out to withdraw and weigh the fetuses and placentas. The maternal and fetal dates were analyzed by Two-way ANOVA followed by t Test. The newborns (NB) were classified in small, appropriate and large for gestational age and the comparisons between the groups were accomplished according to Qui-square Test. Rats STZ presented higher glycemia at days 0 and 14 of pregnancy, lower numbers of alive fetuses; implantations and corpora lutea; increased rate of embryonic losses, placental weight and proportion of small NB (SGA) and large (LGA); reduced rate of AGA NB and unaltered insulin concentrations. Therefore, the mild diabetes altered the maternal glycemia in the early pregnancy, which caused changes in the maternal organism and/or in the early development of the embryo, impairing its implantation and future placental and fetal development.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Gastric motility in pregnant diabetic rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Effect of maternal obesity on diabetes development in adult rat offspring

This study aimed to evaluate whether maternal obesity leads to the onset of diabetes in adult Wistar rats offspring. MSG solution neonatally administration induced obesity in rats (F(1)MSG group, n = 30); and saline solution was also administrated to control rats (F1CON group, n = 13). In 3rd month of age, both control and MS G groups were mated for offspring (generation FA named as F2CON, n = 28 and F(2)MSG groups, n = 15; and so both generations were studied until 7th month of life. Lee Index was measured for experimental obesity validation from 5th to 7th month. Glycemia was weekly determined during pregnancy and monthly from 3rd to 7th month. In the end of experimental period all rats were submitted to oral glucose tolerance test (OGTT), with estimation of total area under the curve (AUC); and insulin tolerance test (ITT). Rats were then anesthetized and killed. Data were statistically analyzed with significance level of p < 0.05. Lee Index has confirmed obesity in all MSG rats. Glycemic levels comparisons between generations showed significant maternal interference in control and MSG groups. OGTT analysis showed higher glycemia in obese rats (F(1)MSG) and their offspring (F(2)MSG) as compared to their respective controls; and MSG groups increased AUC from OGTT. As regards ITT, F(2)MSG showed higher glycemia at 30 and 120 min, suggesting a delay of insulin action decreasing. Although glucose intolerance and insulin resistance clinical conditions represent as a factors for type 2 Diabetes mellitus development, this experimental model proposal was not efficient to induce type 2 Diabetes mellitus, but for obesity developing, glucose intolerance and insulin resistance in successive generations of rats. (c) 2007 Elsevier B.V. All rights reserved