Functional Analysis of a Dominant Negative Mutation of Interferon Regulatory Factor 5

BACKGROUND: Interferon regulatory factor (IRF) family members have been implicated as critical transcription factors that function in immune response, hematopoietic differentiation and cell growth regulation. Activation of IRF-5 results in the production of pro-inflammatory cytokines such as TNFalpha, IL6 and IL12p40, as well as type I interferons. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we identify a G202C (position relative to translation start codon) missense-mutation transcript of IRF-5 in transformed B and T cell lines, which were either infected or non-infected by viruses, and peripheral blood from ATL or CLL patients. The mutated transcript encodes a novel protein in which the sixty-eighth amino acid, Alanine, is substituted by Proline (IRF-5P68) in the DNA binding domain of IRF-5. IRF-5P68 phenotype results in a complete loss of its DNA-binding activity and functions as a dominant negative molecule through interacting with wild type IRF-5. Co-expression of IRF-5P68 inhibits MyD88-mediated IRF-5 transactivation. Moreover, Toll-like receptor (TLR)-dependent IL6 and IL12P40 production induced by lipopolysaccharide (LPS), R837 or CpG ODN 1826 was reduced in IRF-5 (P68) expressing cells as compared to the control cells. CONCLUSION: IRF-5P68 acts as a dominant negative regulator that interferes with IRF-5-mediated production of pro-inflammatory cytokines. The functional characterization of the novel IRF-5 mutant in transformed B and T cell lines and in ATL and CLL patients may lead to a better understanding of the role of these transcriptional regulators in hematopoietic malignancies

Light-harvesting chlorophyll a/b-binding protein-coding genes in jatropha and the comparison with castor, cassava and arabidopsis

The Lhc (light-harvesting chlorophyll a/b-binding protein) superfamily represents a class of antennae proteins that play indispensable roles in capture of solar energy as well as photoprotection under stress conditions. Despite their importance, little information has been available beyond model plants. In this study, we presents a first genome-wide analysis of Lhc superfamily genes in jatropha (Jatropha curcas L., Euphorbiaceae), an oil-bearing plant for biodiesel purpose. A total of 27 members were identified from the jatropha genome, which were shown to distribute over nine out of the 11 chromosomes. The superfamily number is comparable to 28 present in castor (Ricinus communis, Euphorbiaceae), but relatively less than 35 in cassava (Manihot esculenta, Euphorbiaceae) and 34 in arabidopsis (Arabidopsis thaliana) that experienced one or two recent whole-genome duplications (WGDs), respectively. In contrast to a high number of paralogs present in cassava and arabidopsis, few duplicates were found in jatropha as observed in castor, corresponding to no recent WGD occurred in these two species. Nevertheless, 26 orthologous groups representing four defined families were found in jatropha, and nearly one-to-one orthologous relationship was observed between jatropha and castor. By contrast, a novel group named SEP6 was shown to have been lost in arabidopsis. Global transcriptome profiling revealed a predominant expression pattern of most JcLhc superfamily genes in green tissues, reflecting their key roles in photosynthesis. Moreover, their expression profiles upon hormones, drought, and salt stresses were also investigated. These findings not only improve our knowledge on species-specific evolution of the Lhc supergene family, but also provide valuable information for further studies in jatropha

Energy Revolution and Security Guarantee of China’s Energy Economy

Energy is the blood for economic operation. Energy structure adjustment may significantly impact the macroeconomic operation of a country. This study quantitatively estimates the effect of energy structure adjustment on energy prices and further evaluates the impact of the resulted price changes on macroeconomic operations using an econometric model and statistical methods. The results indicate that 1% increase of the proportion of natural gas consumption in the total energy consumption raises energy prices by 0.8% while 1% increase of the coal consumption ratio lowers energy prices by 0.2%. Moreover, technological progress, energy investment growth, increased marketization, and energy efficiency improvement are conducive to reducing energy prices. We conclude that China’s energy revolution is less likely to affect the security of the energy economy and the energy price rise remains within the acceptable range of national economy and energy consumption. To better ensure the security of energy economy in the process of China’s energy revolution, we propose that China should focus on technology innovation and deepen the reforms in the energy market to improve the competitiveness of the energy market. These measures are beneficial for mitigating the energy price increase pressure from energy revolution

Transgenerational Effects of Maternal Water Condition on the Growth, C:N Stoichiometry and Seed Characteristics of the Desert Annual Atriplex aucheri

Water conditions directly affect plant growth and thus modify reproduction allocation. However, little is known about the transgenerational effects of water conditions on xerophytes. The desert annual Atriplex aucheri produces three types of seeds (A: dormant, ebracteate black seeds; B: dormant, bracteolate black seeds; C: non-dormant, bracteolate brown seeds) on a single plant. The aim of this study was to investigate the effects of low/high water treatment (thereafter progeny water treatment) on aboveground biomass, C:N stoichiometry, and offspring seed characteristics of A. aucheri grown from brown seeds whose mother plants were under low/high water treatment (thereafter maternal water treatment). Progeny water only affected shoot dry weight and seed allocation of type A. Under low progeny water treatment, plants from parents with low maternal water treatment had the lowest biomass. Maternal water did not significantly influence the C and N content, however high maternal water increased the C:N ratio. Maternal water treatment did not significantly affect seed number. However, plants under low maternal and progeny water treatments had the lowest weight for type B seeds. When progeny plants were under low water treatment, seed allocation of type A, type B, and total seed allocation of plants under high maternal water were significantly lower than those of plants under low maternal water. These results indicate that water conditions during the maternal generation can dramatically contribute to progeny seed variation, but the transgenerational effects depend on the water conditions of progeny plants

Transgenerational Effects of Maternal Water Condition on the Growth, C:N Stoichiometry and Seed Characteristics of the Desert Annual <i>Atriplex aucheri</i>

Water conditions directly affect plant growth and thus modify reproduction allocation. However, little is known about the transgenerational effects of water conditions on xerophytes. The desert annual Atriplex aucheri produces three types of seeds (A: dormant, ebracteate black seeds; B: dormant, bracteolate black seeds; C: non-dormant, bracteolate brown seeds) on a single plant. The aim of this study was to investigate the effects of low/high water treatment (thereafter progeny water treatment) on aboveground biomass, C:N stoichiometry, and offspring seed characteristics of A. aucheri grown from brown seeds whose mother plants were under low/high water treatment (thereafter maternal water treatment). Progeny water only affected shoot dry weight and seed allocation of type A. Under low progeny water treatment, plants from parents with low maternal water treatment had the lowest biomass. Maternal water did not significantly influence the C and N content, however high maternal water increased the C:N ratio. Maternal water treatment did not significantly affect seed number. However, plants under low maternal and progeny water treatments had the lowest weight for type B seeds. When progeny plants were under low water treatment, seed allocation of type A, type B, and total seed allocation of plants under high maternal water were significantly lower than those of plants under low maternal water. These results indicate that water conditions during the maternal generation can dramatically contribute to progeny seed variation, but the transgenerational effects depend on the water conditions of progeny plants

Synthesis, growth mechanism of different Cu nanostructures and their application for non-enzymatic glucose sensing

Cu nanostructures with different shapes were synthesized via a simple reduction approach from CuO nanoleaves at room temperature. The purity and morphology of samples were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) measurements. By changing the dosage of reducing agent, Cu flowers and Cu octahedral cages were obtained successfully. The growth mechanism of morphology evolvement to different shaped Cu nanostructures is discussed. Thermo-gravimetric and differential thermal analysis (TG-DTA) results illuminated that Cu flowers sample has relatively higher activity to oxygen in air atmosphere compared to Cu octahedral cages. A well-defined, stable and fast amperometric response of glucose was observed when the Cu nanostructures were constructed as nonenzymatic glucose sensors. The Cu flowers modified electrode has higher sensitivity than Cu octahedral cages modified one. (C) 2012 Elsevier B.V. All rights reserved

<i>Picea schrenkiana</i> tree ring blue intensity reveal recent glacier mass loss in High Mountain Asia is unprecedented within the last four centuries

Studies on long-term fluctuations in glacier volume and mass are crucial for understanding past climate change. In this paper, we utilized Picea schrenkiana to develop a 525-year chronology of latewood blue intensity (LWBI) in the Tianshan Mountains. Relying on temperature as the main controlling factor for tree growth and glacier mass balance (GMB) variations, the LWBI chronology was used to reconstruct the summer temperature (JJA, R2adj = 47%) and the annual glacier mass balance (annual GMB, R2adj = 39%) in the Tianshan Mountains over the past 400 years. The reconstruction results show that the rapid warming since 1974 has caused the Tianshan No.1 glacier (TS No.1) to experience an unprecedented melting trend within the last four centuries. It is disturbing that the glacier still remain in an ablation state for the next 80 years under both representative concentration paths (RCP) 4.5 and 8.5 scenarios, which will exacerbate the adverse environmental impacts of glacial hazards. Our study provides a continuous record for glacier research in high mountains Asian and contributes to a more detailed assessment of glacier and climate change in this region

Qingnao dripping pills mediate immune-inflammatory response and MAPK signaling pathway after acute ischemic stroke in rats

The aim of the present study was to examine the neuroprotective effect of Qingnao dripping pills (QNDP), especially the infiltration of neutrophils and macrophages, as well as the mitogen-activated protein kinase (MAPK) signal pathway. Adult male Sprague–Dawley rats were randomized to three groups: sham, MCAO, and QNDP. After 24 h of ischemia and reperfusion, neurological deficit scores and infarct volume were measured. Macrophages and neutrophil infiltration in the ischemic brain were respectively determined with CD68 and MPO immunofluorescence and western blot. The proteins involved in MAPK signaling (SAPK/JNK, P-SAPK/JNK, p38, P-p38, ERK1/2, and P-ERK1/2) were measured by western blotting. In vitro ischemic paradigm (oxygen-glucose deprivation) was performed in SH-SY5Y cells to evaluate the effects of QNDP. The viability and death ration of cells induced OGD/R was measured by MTT and LDH assay. The proteins involved in MAPK signaling were measured by western blotting. The results showed that QNDP treatment significantly improved the neurological deficit scores and reduced infarct size. In addition, QNDP treatment inhibited the number of CD68- and MPO-positive cells in the ischemic brain. It inhibited the MAPK signaling pathway in the ischemic brain and SH- SY5Y cells induced OGD/R. Keywords: Acute ischemic stroke, QNDP, Immune-inflammatory response, MAPK signaling pathway, MCA