56 research outputs found
One In-Situ Extraction Algorithm for Monitoring Bunch-by-Bunch Profile in the Storage Ring
As the brightness of synchrotron radiation (SR) light sources improves, the
operation stability of light sources is weakened. To explore various beam
instability related issues in light sources, one transverse beam diagnostics
system for bunch-by-bunch (BbB) profile measurement has been established at
Hefei Light Source-II (HLS-II). In this paper, one in-situ extraction algorithm
in the data processing backend of the system is developed for BbB profiles, so
as to provide important beam information of the machine operation in time.Comment: Accepted by the International Conference on Optical Communication and
Optical Information Processing (OCOIP 2023
Preliminary X-ray crystallographic studies of mouse UPR responsive protein P58(IPK) TPR fragment
Identification of residues involved in nucleotidyltransferase activity of JHP933 from helicobacter pyloriby site-directed mutagenesis
Helicobacter pylori is a well-known bacterial pathogen involved in the
development of peptic ulcer, gastric adenocarcinoma and other forms of
gastric cancer. Evidence has suggested that certain strain-specific genes in
the plasticity region may play key roles in the pathogenesis of H.
pylori-associated gastroduodenal diseases. Therefore there is considerable
interest in the strain-specific genes located in the plasticity regions of H.
pylori. JHP933 is encoded by the gene in the plasticity region of H. pylori
strain J99. Recently, the crystal structure of JHP933 has confirmed it as a
nucleotidyltransferase (NTase) superfamily protein and a putative active site
has been proposed. However, no evidence from direct functional assay has been
presented to confirm the active site and little is known about the functional
mechanism of JHP933. Here, through superimposition with Cid1/NTP complex
structures, we modelled the complex structures of JHP933 with different NTPs.
Based on the models and using rational site-directed mutagenesis combined
with enzymatic activity assays, we confirm the active site and identify
several residues important for the nucleotidyl transferring function of
JHP933. Furthermore, mutations of these active site residues result in the
abolishment of the nucleotidyltransferase activity of JHP933. This work
provides preliminary insight into the molecular mechanism underlying the
pathophysiological role in H. pylori infection of JHP933 as a novel NTase
superfamily protein
Effect of Polyimide-Phosphating Double Coating and Annealing on the Magnetic Properties of Fe-Si-Cr SMCs
Fe-Si-Cr soft magnetic powder cores (SMCs), with high electrical resistivity, magnetic permeability, saturation magnetic induction, and good corrosion resistance, are widely applied to inductors, filters, choke coils, etc. However, with the development of electronic technology with high frequency and high power density, the relative decline in the magnetic properties limits the high-frequency application of SMCs. In this paper, the phosphating process and polyimide (PI) insulation coating is applied to Fe-Si-Cr SMCs to reduce the core loss, including hysteresis loss and eddy current loss. The microstructure and composition of Fe-Si-Cr powders were analyzed by SEM, XRD, and Fourier-transform infrared spectra, respectively. The structural characteristics of the Fe-Si-Cr @ phosphate layer @ PI layer core–shell double coating were studied, and the best process parameters were determined through experiments. For SMCs with 0.4 wt% content of PI, the relative permeability is greater than 68%, and the core loss is the lowest, 7086 mW/cm3; annealed at 500 °C, the relative permeability is greater than 57%, and the core loss is the lowest, 6222 mW/cm3. A 0.4 wt% content of PI, annealed at 500 °C, exhibits the ideal magnetic properties: μe = 47 H/m, P = 6222 mW/cm3
Preliminary X-ray crystallographic studies of yeast mitochondrial protein Tom70p
Tom70p is an important translocase of the outer membrane complex member and a major surface receptor of the protein-translocation machinery in the outer mitochondrial membrane. To investigate the mechanism by which Tom70p functions to deliver the mitochondrial protein precursors, the cytosolic fragment of yeast Tom70p (cTom70p) has been crystallized
Data on the phylogenetic typing, integron gene cassette array analysis, multi-drug resistance analysis and correlation between antimicrobial resistance determinants in Klebsiella strains
The antimicrobial resistance of Klebsiella species in the poultry industry is becoming a public concern. In support our recent publication “Characterization of antimicrobial resistance in Klebsiella species isolated from chicken broilers” (Wu et al., 2016) [1], multilocus sequence typing (MLST) and gyrA PCR-RFLP assays were conducted to identify the genetic relationships between and phylogenetic groups of the 90 antimicrobial resistant Klebsiella species isolated from a commercial broiler slaughter plant in Shandong, China. In addition, PCR-RFLP was performed to identify different gene cassette arrays in class 1 and 2 integrons, and the correlations between different antimicrobial resistance determinants were analyzed. Keywords: Antimicrobial resistant Klebsiella species, Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), Gene cassette arrays of integron, Statistical analysi
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