“Output Research Supporting Class” Manuscript Writing Training to Avoid Misconduct in an Islamic Perspective

The tridharma point that is most challenging for a lecturer to achieve is research, and currently, the benchmark for the research field is scientific publications. However, in reality, there are still many lecturers who are hampered in fulfilling research outputs in the form of scientific publications, this is because lecturers experience difficulties in compiling manuscripts of research results for publication. There are currently 144 lecturers at the Tasikmalaya Health Engineering Polytechnic and around 50% of the lecturers still have the functional position of expert assistant and do not have a functional position or teaching staff. This PKM aims to improve the ability of Tasikmalaya Health Polytechnic lecturers to compile manuscripts, avoid misconduct, and publish the manuscript in the intended journal. PKM methodThis is done using an Asset Based Community-Driven Development (ABCD) approach, namely focusing on the assets, potential and strengths of the Health Polytechnic lecturers. Workshop and coaching clinic preparing manuscripts, using reference management, and submitting manuscripts to journals. The research results showed that of the 50 participants who took part in the workshop, 15 lecturers succeeded in compiling manuscripts of research results, avoiding misconduct, and improving the manuscripts according to reviewer input. This shows the need for ongoing training and stimulation activities for lecturers in compiling research output

Halal Critical Point Analysis of <i>Bajakah</i> Wood (<i>Spatholobus littoralis</i> Hassk.) Nano Particle as Anticancer Agent

Bajakah wood contains phenolic compounds, flavonoids, tannins, and saponins with anticancer activity. The discovery and development of new drugs require several stages. In the process, there are many possibilities of adding other substances to form new active substances or as solvents that allow drug preparations to be doubtful of halalness. Hence, it is necessary to analyze the critical point of halal ingredients. The purpose of this study was to determine the characteristics of the nanoparticles of bajakah wood (Spatholobus littoralis Hassk.) and to test the anticancer activity in several cancer cell cultures, as well as to analyze the critical point of halalness of the material. This research method is a composition test using chromatography and anticancer activity test using MTT. Analysis of the critical point of halal materials using hazard analysis critical control point (HACCP). The research was carried out at the Indonesian Engineering Nanotechnology Laboratory South Tangerang and the UGM Integrated Laboratory Sleman in July–December 2020. The results showed that bajakah wood nanoparticles contained pure water as a solvent, viscosity 0.08878 cP, scattering intensity 1.1059 cps, diameter 176.1+/−43.7 (nm). Cytotoxic test results showed IC50 against cell culture MCF7 1,063.28 (±114.98) g/mL, HepG2 53.34 (±0.35) g/mL, T47D 150.63 (±8.44) g/mL, WiDR 114.38 (±7.82) μg/mL, HTB 97.50 (±3.49) μg/mL, HeLa 182.95 (±36.22) μg/mL, and Vero 710.10 (±106.46) μg/mL. This study concludes that bajakah wood nanoparticles are not critical in terms of halal ingredients. At the same time, their anticancer activity is weak against breast cancer and uterine cervical cancer, medium categories against liver cancer and lung cancer, and is not toxic to normal cells.   ANALISIS TITIK KRITIS KEHALALAN NANOPARTIKEL KAYU BAJAKAH (SPATHOLOBUS LITTORALIS HASSK.) SEBAGAI AGEN ANTIKANKER Kayu bajakah mengandung senyawa fenolik, flavonoid, tanin, dan saponin yang memiliki aktivitas antikanker. Penemuan dan pengembangan obat baru memerlukan beberapa tahapan. Dalam prosesnya terdapat banyak kemungkinan penambahan zat lain untuk membentuk zat aktif baru atau sebagai pelarut yang memungkinkan sediaan obat diragukan kehalalannya. Oleh sebab itu, perlu dilakukan analisis titik kritis kehalalan bahan. Tujuan penelitian ini mengetahui karakteristik sediaan nanopartikel kayu bajakah (Spatholobus littoralis Hassk.) dan menguji aktivitas antikanker pada beberapa kultur sel kanker, serta menganalisis titik kritis kehalalan bahan. Metode penelitian ini adalah uji komposisi menggunakan kromatografi dan uji aktivitas antikanker menggunakan MTT. Analisis titik kritis kehalalan bahan menggunakan hazard analysis critical control point (HACCP). Penelitian dilakukan di Laboratorium Nanovasi Rekayasa Indonesia Tangerang Selatan dan Laboratorium Terpadu UGM Sleman pada Juli–Desember 2020. Hasil penelitian menunjukkan sediaan nanopartikel kayu bajakah mengandung pelarut air murni, viskositas 0,08878 cP, scattering intensity 1,1059 cps, berdiameter 176,1+/−43,7 (nm). Hasil uji sitotoksik menunjukkan IC50 terhadap kultur sel MCF7 1.063,28 (±114,98) μg/mL, HepG2 53,34 (±0,35) μg/mL, T47D 150,63 (±8,44) μg/mL, WiDR 114,38 (±7,82) μg/mL, HTB 97,50 (±3,49) μg/mL, HeLa 182,95 (±36,22) μg/mL, dan Vero 710,10 (±106,46) μg/mL. Kesimpulan penelitian ini bahwa nanopartikel kayu bajakah bersifat tidak kritis dalam kehalalan bahan. Selain itu, aktivitas antikankernya lemah terhadap kanker payudara dan kanker serviks uteri, sedang terhadap kanker hati dan kanker paru, serta tidak toksik pada sel normal

Antagonic Effect of Soursop Leaf Aqueous Extract and Doxorubicin Combination in MCF7 and T47D Breast Cancer Cell

The success of breast cancer therapy is still not optimal and the side effects caused by breast cancer therapy. The use of standard drug combinations with herbs is often used as co-chemotherapy and is believed to increase the drug's effectiveness. However, research on the antagonistic effect of the drug combination is still minimal. This study examines the anticancer effect of soursop leaf aquoxes extract and the combined impact of doxorubicin on MCF7 and T47D breast cancer cells. This research is pure in vitro experimental study of MCF7 and T47D breast cancer culture cells at the Parasitology Laboratory of the Faculty of Medicine, Universitas Gadjah Mada in August 2018. Toxicity tests were carried out using the method of tetrazolium 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) to calculate cell viability. The IC50 value was obtained by analyzing probit regression calculation using SPSS software. The synergism of this compound with doxorubicin was determined based on the value of the Combination Index (CI) using a combination test with series 1/2 IC50, 3/8 IC50, 1/4 IC50, and 1/8 IC50 and the data was analyzed using Compusyn 1.0 software. In this study, the effect of soursop leaf preparations will be tested on T47D and MCF7 breast cancer cell cultures and assess the impacts of co-chemotherapy of soursop leaf aqueous extract with doxorubicin. This study showed that IC50 soursop leaf aqueous extract in T47D breast cancer culture was 84 μg/mL and in MCF7 166.5 μg/mL. In contrast, the combined test showed that soursop leaf aqueous extract was antagonistic with doxorubicin in both T47D and MCF7 cancer cell cultures.   EFEK ANTAGONIS KOMBINASI EXTRAK AIR DAUN SIRSAK DAN DOKSORUBISIN PADA KULTUR SEL KANKER MCF7 AND T47D Keberhasilan terapi kanker payudara saat ini masih belum optimal dan terdapat efek samping yang ditimbulkan dari terapi kanker payudara tersebut. Penggunaan kombinasi obat standar dengan herbal sering digunakan sebagai ko-kemoterapi dan diyakini dapat meningkatkan efektivitas obat, tetapi penelitian mengenai efek antagonis kombinasi obat masih sangat terbatas. Penelitian ini mengkaji efek antikanker ekstrak air daun sirsak dan kombinasinya dengan doksorubisin pada sel kanker payudara MCF7 dan T47D. Penelitian ini merupakan eksperimental murni secara in vitro pada sel kanker payudara MCF7 dan T47D di Laboratorium Parasitologi Fakultas Kedokteran Universitas Gadjah Mada periode Agustus 2018. Uji toksisitas dilakukan menggunakan metode tetrazolium 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) untuk menghitung viabilitas sel. Nilai IC50 didapatkan melalui analisis menggunakan perhitungan regresi probit menggunakan perangkat lunak SPSS. Efek sinergis senyawa ini dengan doksorubisin ditentukan berdasar atas nilai Indeks Kombinasi (IK) menggunakan uji kombinasi dengan seri 1/2 IC50, 3/8 IC50, 1/4 IC50, dan 1/8 IC50 serta data dianalisis menggunakan perangkat lunak Compusyn 1.0. Efek sediaan daun sirsak pada penelitian ini akan diujikan terhadap kultur sel kanker payudara T47D dan MCF7 serta menilai efek ko-kemoterapi ekstrak air daun sirsak dengan doksorubisin. Hasil penelitian ini menunjukkan bahwa IC50 ekstrak air daun sirsak pada kultur sel kanker T47D adalah 84 μg/mL dan pada kultur sel kanker MCF7 166.5 μg/mL, sedangkan uji kombinasi memperlihatkan bahwa ekstrak air daun sirsak berefek antagonis dengan doksorubisin pada kultur sel kanker T47D dan MCF7

Potensi Ekstrak Air Daun Sirsak sebagai Penurun Kolesterol dan Pengendali Bobot Badan

Penelitian ini bertujuan untuk mengetahui efek ekstrak air daun sirsak terhadap bobot badan dan kadar kolesterol darah. Penelitian ini menggunakanrancangan acak lengkap dengan 5 kelompok perlakuan masing-masing sebanyak 3 ulangan. Hewan coba berupa tikus galur Wistar sebanyak 15 ekor dibagi menjadi 5 kelompok perlakuan, yaitu kelompok yang diberikan ekstrak air daun sirsak dengan dosis 200 mg/kgBB, 400mg/kgBB, kontrol positif, kontrol negatif, dan kontrol normal. Rerata bobot badan dan kadar kolesterol kemudian dianalisis menggunakan Sapphiro Wilk test, ANOVA dan Kruskall Wallis. Hasil penelitian menunjukkan bahwa semua kelompok yang diberikan ekstrak daun sirsak memiliki efek menghambat peningkatan bobot badan jika dibandingkan dengan kontrol normal, sedangkan untuk kadar kolesterol darah didapatkan bahwa seluruh kelompok perlakuan menunjukkan kadar kolesterol darah yang sama dengan kelompok yang diberikan simvastatin. Kesimpulan penelitian ini adalah bahwa ekstrak air daun sirsak memiliki efek mengendalikan bobot badan dan kolesterol darah. Efek terhadap kolesterol darah serupa dengan simvastatin, karena ekstrak air daun sirsak mengandung fl avonoid yang mempunyai efek menghambat enzim HMG CoA reduktase, serupa dengan mekanisme kerja simvastatin dalam menurunkan kadar kolesterol darah

Clinical Characteristics, Comorbidities, Length of Stay, and Mortality of COVID-19 Patients in RSUD Cideres, Majalengka, West Java

Until now, the world is still facing the COVID-19 pandemic caused by SARS-CoV-2. The number of deaths of COVID-19 patients in Indonesia is quite large when compared to other countries. Clinical manifestations, comorbidities, length of stay, and the mortality of COVID-19 vary widely in each study. This study analyzes clinical characteristics, comorbidities, length of stay, and mortality of COVID-19 patients in RSUD Cideres, Majalengka, West Java. This study is an observational study with total sampling and purposive sampling. Subjects were patients with nasal swabs confirmed SARS-CoV-2 and hospitalized at RSUD Cideres from January to July 2021. Clinical manifestations, comorbidities, length of stay, and mortality of COVID-19 patients aged ≥17 years old, were extracted from medical records. Results showed that the majority of 213 COVID-19 patients were age 41–60 years 114 (53.5%), female 114 (53.5%), with an upper respiratory tract in 207 (97.1%), and shortness of breath in 203 (95.3%) patients. 153 (71.8%) patients had a fever, while headache, diarrhea, and anosmia respectively 49 (23.0%), 19 (8.9%), and 10 (4.6%), respectively. Vital signs were the Glasgow Coma Scale (GCS) normal in 190 (89.2%) patients. Most of patients had respiration rate >20/min in 201 (94.3%), oxygen saturation <90% in 98 (46.0%), and chest x-rays: bronchopneumonia 134 (62.9%) and pneumonia 23 (10.7%). Comorbidities were hypertension, diabetes mellitus, and chronic heart failure in 74 (34.7%), 56 (26.2%), and 46 (11.7%) patients, respectively. The length of stay who died for 1–7 days (22 patients) and 8–14 days (9 patients); means almost all patients (29 of 32) died during the length of stay for 1–14 days. In conclusion, the majority of COVID-19 patients at RSUD Cideres are female, aged 41–60 years, had upper respiratory tract/shortness of breath, GCS normal, saturation oxygen <90%, chest x-rays: bronchopneumonia and pneumonia, comorbidities: hypertension, and length of stay for patients who died 1–7 days.   KARAKTERISTIK KLINIS, KOMORBITAS, LAMA RAWAT, DAN MORTALITAS PASIEN COVID-19 DI RSUD CIDERES, MAJALENGKA, JAWA BARAT Hingga saat ini, dunia masih menghadapi pandemi COVID-19 yang disebabkan oleh SARS-CoV-2. Jumlah kematian pasien COVID-19 di Indonesia cukup besar jika dibanding dengan negara lain. Manifestasi  klinis, komorbid, lama rawat, dan mortalitas pasien COVID-19 sangat bervariasi pada setiap penelitian. Tujuan penelitian ini menganalisis karakteristik klinis, komorbiditas, lama rawat, dan mortalitas pasien COVID-19 di RSUD Cideres, Majalengka, Jawa Barat. Penelitian ini adalah penelitian observasional dengan total sampling dan purposive sampling. Subjek adalah pasien dengan nasal swab terkonfirmasi SARS-CoV-2 dan dirawat di RSUD Cideres periode Januari hingga Juli 2021. Data manifestasi klinis, komorbid, lama rawat, dan mortalitas pasien COVID-19 usia ≥17 diambil dari rekam medik. Hasil menunjukkan bahwa mayoritas 213 pasien COVID-19 berusia 41–60 tahun 114 (53,5%) dan wanita 114 (53,5%) dengan keluhan saluran napas atas 207 (97,1%) dan napas pendek 203 (95,3%) pasien. 153 (71,8%) pasien mengalami demam, sedangkan sakit kepala, diare, dan anosmia masing-masing 49 (23,0%), 19 (8,9%), dan 10 (4,6%) pasien. Tanda vital Glasgow Coma Scale (GCS) normal pada 190 (89,2%) pasien. Kebanyakan pasien mempunyai frekuensi napas >20/menit pada 201 (94,3%) pasien, saturasi oksigen <90% pada 98 (46,0%) pasien, serta rontgen toraks: bronkopneumonia pada 134 (62,9%) pasien dan pneumonia pada 23 (10,7%) pasien. Komorbid adalah hipertensi, diabetes melitus, dan chronic heart failure pada 74 (34,7%), 56 (26,2%), dan 46 (11,7%) pasien berturut-turut. Lama rawat pasien yang meninggal 1–7 hari (22 pasien) dan 8–14 hari (9 pasien), berarti hampir semua pasien (29 dari 32) meninggal selama rawat 1–14 hari. Simpulan, mayoritas pasien COVID-19 di RSUD Cideres adalah wanita, berusia 41–60 tahun, keluhan saluran napas atas/napas pendek, GCS normal, rontgen toraks: bronkopneumonia dan pneumonia, komorbid: hipertensi, serta lama rawat pasien yang meninggal paling banyak 1–7 hari

“Output Research Supporting Class” Manuscript Writing Training to Avoid Misconduct in an Islamic Perspective

The tridharma point that is most challenging for a lecturer to achieve is research, and currently, the benchmark for the research field is scientific publications. However, in reality, there are still many lecturers who are hampered in fulfilling research outputs in the form of scientific publications, this is because lecturers experience difficulties in compiling manuscripts of research results for publication. There are currently 144 lecturers at the Tasikmalaya Health Engineering Polytechnic and around 50% of the lecturers still have the functional position of expert assistant and do not have a functional position or teaching staff. This PKM aims to improve the ability of Tasikmalaya Health Polytechnic lecturers to compile manuscripts, avoid misconduct, and publish the manuscript in the intended journal. PKM methodThis is done using an Asset Based Community-Driven Development (ABCD) approach, namely focusing on the assets, potential and strengths of the Health Polytechnic lecturers. Workshop and coaching clinic preparing manuscripts, using reference management, and submitting manuscripts to journals. The research results showed that of the 50 participants who took part in the workshop, 15 lecturers succeeded in compiling manuscripts of research results, avoiding misconduct, and improving the manuscripts according to reviewer input. This shows the need for ongoing training and stimulation activities for lecturers in compiling research output