A Target Site for Spontaneous Insertion of IS10 Element in pUC19 DNA Located within Intrinsically Bent DNA

Residual insertion sequence elements (IS elements) in Escherichia coli strains that are commonly used for DNA cloning are known to cause cloning artifacts by transposing themselves into the recombinant DNA fragments. In such cases, chance insertion of IS elements may occur at integration sites in the cloning targets, which in the case of the IS10 element is a 9-bp consensus sequence. We report here that the integration of IS10-related DNA sequences into the pUC19 cloning vector and its derivative occurred with considerable frequency in E. coli strains JM107 and DH10B, with duplication of a 9-bp segment (TCTAAAGTA). Notably, native polyacrylamide gel electrophoresis revealed that intrinsically bent DNA flanks the insertion site

Bethe ansatz for Yangian invariants: Towards super Yang–Mills scattering amplitudes

We propose that Baxter's Z -invariant six-vertex model at the rational gl(2) point on a planar but in general not rectangular lattice provides a way to study Yangian invariants. These are identified with eigenfunctions of certain monodromies of an auxiliary inhomogeneous spin chain. As a consequence they are special solutions to the eigenvalue problem of the associated transfer matrix. Excitingly, this allows to construct them using Bethe ansatz techniques. Conceptually, our construction generalizes to general (super) Lie algebras and general representations. Here we present the explicit form of sample invariants for totally symmetric, finite-dimensional representations of gl(n) in terms of oscillator algebras. In particular, we discuss invariants of three- and four-site monodromies that can be understood respectively as intertwiners of the bootstrap and Yang–Baxter equation. We state a set of functional relations significant for these representations of the Yangian and discuss their solutions in terms of Bethe roots. They arrange themselves into exact strings in the complex plane. In addition, it is shown that the sample invariants can be expressed analogously to Graßmannian integrals. This aspect is closely related to a recent on-shell formulation of scattering amplitudes in planar N=4 super Yang–Mills theory

Activity and stability of recombinant human superoxide dismutase in buffer solutions and hypothermic perfusates.

The stability of recombinant human superoxide dismutase (r-hSOD) in buffer solutions was studied in solutions at various pH and temperatures. Additionally, we studied the effects of incubation with proteases, serum and two types of hypothermic perfusates. R-hSOD was stable in the pH range of 6-11 and at temperatures up to 80 degrees C for 30 min. R-hSOD activity was not affected by incubation with trypsin, aminopeptidase M or serum for 2 h. R-hSOD activity determined at various temperatures (4-37 degrees C) did not vary remarkably. R-hSOD in hypothermic perfusates was stable at 4-37 degrees C for 24 h.</p

Self-bound dense objects in holographic QCD

We study a self-bound dense object in the hard wall model. We consider a spherically symmetric dense object which is characterized by its radial density distribution and non-uniform but spherically symmetric chiral condensate. For this we analytically solve the partial differential equations in the hard wall model and read off the radial coordinate dependence of the density and chiral condensate according to the AdS/CFT correspondence. We then attempt to describe nucleon density profiles of a few nuclei within our framework and observe that the confinement scale changes from a free nucleon to a nucleus. We briefly discuss how to include the effect of higher dimensional operator into our study. We finally comment on possible extensions of our work.Comment: 17 pages, 5 figures, figures replaced, minor revision, to appear in JHE

The Entropy Function for the extremal Kerr-(anti-)de Sitter Black Holes

Based on the entropy function formalism, we consider the extremal Kerr-(anti-)de Sitter black holes in 4-dimensions. Solving differential equations exactly, which are obtained by extremizing the entropy function, we find agreement of the result with Bekenstein-Hawking entropy. Concerning the higher derivative corrections, we extend the computation to the case with Gauss-Bonnet term.Comment: 24 pages, 4 figures, comments on Gauss-Bonnet coefficient added, references adde

Effect of Implantable Cardioverter-defibrillator Therapy for Ventricular Fibrillation Patients with Out-of-hospital Cardiac Arrest

Survivors of ventricular fibrillation out-of-hospital cardiac arrest（VF-OHCA）due to potentially reversible causes such as acute coronary syndrome（ACS）, vasospastic angina and electrolyte abnormalities are considered low risk for recurrent arrhythmia. Accordingly, implantable cardioverter-defibrillator therapy is not routinely recommended in such patients. We investigated the risk of mortality and the value of ICD therapy for VF-OHCA. Among 2,248 cardiopulmonary arrest patients presenting at our hospital, we retrospectively investigated 110 patients with VF-OHCA who were admitted for treatment. We divided the patients based on ICD（n＝71） or No-ICD status（n＝39）, and on reversible cause（n＝70） or irreversible cause（n＝40）. The groups were compared for baseline characteristics, mortality and ICD therapies. Patients with a reversible cause had a significantly lower rate of ICD implantation than those with an irreversible cause（P＝0.03）. Males in the ICD group presented more frequently than those in the No-ICD group, and cardiac mortality in patients with acute coronary syndrome was significantly lower with ICD therapy than without ICD（P＝0.04）. The rate of appropriate ICD therapies with a reversible cause was 28％, and the first ICD therapy was highest within 1 year post-implantation. Patients with VF-OHCA due to a reversible cause remain at high risk of recurrent ventricular arrhythmias, suggesting that ICD implantation is a reasonable approach in such cases

Antibody stabilization for thermally accelerated deep immunostaining

Antibodies have diverse applications due to their high reaction specificities but are sensitive to denaturation when a higher working temperature is required. We have developed a simple, highly scalable and generalizable chemical approach for stabilizing off-the-shelf antibodies against thermal and chemical denaturation. We demonstrate that the stabilized antibodies (termed SPEARs) can withstand up to 4 weeks of continuous heating at 55 °C and harsh denaturants, and apply our method to 33 tested antibodies. SPEARs enable flexible applications of thermocycling and denaturants to dynamically modulate their binding kinetics, reaction equilibrium, macromolecular diffusivity and aggregation propensity. In particular, we show that SPEARs permit the use of a thermally facilitated three-dimensional immunolabeling strategy (termed ThICK staining), achieving whole mouse brain immunolabeling within 72 h, as well as nearly fourfold deeper penetration with threefold less antibodies in human brain tissue. With faster deep-tissue immunolabeling and broad compatibility with tissue processing and clearing methods without the need for any specialized equipment, we anticipate the wide applicability of ThICK staining with SPEARs for deep immunostaining

Head-up Tilt Test May Be Useful for Determining Permission of Flight for Regular Passenger Aircraft Pilots with Vasovagal Syncope

To determine the permissibility of piloting an aircraft with vasovagal syncope （VVS） is a complex responsibility, because it is difficult to evaluate VVS quantitatively. There are no clear guidelines for aircraft pilots with VVS. In some facilities, Head-up Tilt Test （HUTT） is used to determine its reproducibility and treatment effect. In most cases, permission is dependent on the strict examinations by specialists and judgement of the committee established for each country. Therefore, we assessed pilots with VVS and designed an algorism of permission for piloting an aircraft. Here, we describe 7 consecutive regular passenger aircraft pilots with VVS who were restricted to fly. All patients were men and their mean age was 37 years. All pilots were permitted to fly after at least two or more tilt-tests. The observation period was two years after flight permission. None of the pilots in the present study fainted after receiving flight permission. We can make a risk stratification to determine hypotensive susceptibility by multiple tilt tests. Based on the results of multiple HUTT, we can more quantitatively make the judgement as to whether the pilot’s VVS is well managed