Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor of HIV-1 replication by functional cDNA library screening in MT-4 cells

AbstractWe conducted a phenotypic cDNA screening using a T cell line-based assay to identify human genes that render cells resistant to human immunodeficiency virus type 1 (HIV-1). We isolated potential HIV-1 resistance genes, including the carboxy terminal domain (CTD) of bromodomain-containing protein 4 (Brd4). Expression of GFP-Brd4-CTD was tolerated in MT-4 and Jurkat cells in which HIV-1 replication was markedly inhibited. We provide direct experimental data demonstrating that Brd4-CTD serves as a specific inhibitor of HIV-1 replication in T cells. Our method is a powerful tool for the identification of host factors that regulate HIV-1 replication in T cells

視野左方偏倚が線分二等分試験に及ぼす影響 : Head Mounted Displayを用いた若年健常者に対する検討

Objective: The purpose of this study was to verify the effect on spatial perception in healthy young subjects of an unconscious leftward optical shift created by a head-mounted display (HMD) with an offset camera. Methods: We recruited 40 healthy right-handed adults who were divided into four groups according to the hand used in the tests and the visual direction displayed by the HMD (centered or 10° left). Each of the four groups (n = 10) undertook line bisection tasks across four combinations of variables: using a finger/stick or a mouse to point at a touch panel located 60 or 120 cm away from the subject. Results: According to the results, regardless of the hand used, when the index finger or a stick was used (reaching condition), the line bisection point was displaced significantly to the left of the center. Additionally, a major left-displacement trend was observed in the short-distance reaching task, which did not require the use of a stick. In contrast, the long-distance task required a stick to be used, and the left displacements were all smaller than those for the short-distance tasks that used the index finger. Conclusion: This finding may be explained by the subjects having sufficient experience coordinating hand and eye movements in the condition where they used their dominant hand and reached with their own arms without using a stick.東京都立大学学位論文甲第1160号　副論

キャリア ケイセイ シエン キョウイク プログラム ノ カイハツ ト キョウイク コウカ ノ ヒョウカ : チーム イリョウ タイケン ガクシュウ ノ ジッセンテキ ケンキュウ オ トオシテ

我々は歯学部学生のキャリア形成支援のための教育プログラム「チーム医療体験学習」を開発し， 歯学部の2，3，4年次の学生を対象として実施した。本プログラムは，グループワークによる事前学 習とチーム医療の現場体験を2つの柱とし，学生の歯科医療職としてのキャリア意識を強化することを 目的とした。プログラム終了時のアンケートから，学生には歯科専門職としての将来の姿勢や目指す方 向性が具体化すると同時に幅広い知識を身につけたいといった学習に対する動機付が強化されるなど 明らかな教育成果が認められた。プログラム実施前後のアンケートの分析結果から， 「チーム医療体験 学習」が歯学部学生のキャリア形成支援教育の手法として有効なプログラムである可能性が示唆された。We prepared career advancement program “Experience learning in medical team treatment" towards the second，third and fouth year students of dental school. This program consits of prior learning concerned with 'Team treatment and Dental profession' and experience learning in medical team treatment at Tokushima University Hospital. The purpose of this program was to rouse career conciousness as a dental profession. This program provided students with career image which they want to be in the future. Besides，they were motivated to study more widely to approach their own image. In this paper，we evaluated the effects of the program“The experience learning in medical team treatment" on career advancement of dental school students. As a result，it is suggested that this program might be fruitful as career advancement on dental students

Simple Stratification of Hepatocellular Carcinoma Surveillance after Direct-acting Antiviral Therapy for Chronic Hepatitis C

Reports on surveillance systems useful for determining the risk of developing hepatocellular carcinoma （HCC） after direct-acting antiviral （DAA） treatment for hepatitis C have been published. Liver cirrhosis （LC） is a high-risk factor for HCC, but the evaluation frequency necessary for patients with chronic hepatitis （CH） remains unknown. Here, we aimed to identify how frequent CH patients should be evaluated for HCC, with particular emphasis on patients achieving a sustained virological response （SVR） with DAA treatment. Data were collected pre-treatment （Pre） and at the time of SVR for 141 patients with hepatitis C receiving DAA treatment. We defined LC by a platelet （PLT） count ≤10×104/µl, and CH was defined by a PLT count of ＞10×104/µl. The incidence of HCC in patients with CH after achieving SVR was retrospectively evaluated. In total, 128 patients （CH, n＝102; LC, n＝26） achieved SVR, and 13 developed HCC after SVR during the follow-up period （mean, 748 days）. Although fibrosis-4 （FIB-4） index, the presence of α-fetoprotein, and prothrombin time were significant risk factors for HCC in patients with CH in the univariate analysis, only the Pre-FIB-4 index was an independent predictive factor for HCC development in the multivariate analysis （p＝0.04）. An FIB-4 index ≥3 was a significant risk factor for HCC （p＝0.005）. The cumulative risk for HCC at 1000 days was 2.6％ and 24.2％ in the FIB-4 index ＜3 and FIB-4 index ≥3 groups, respectively （p＝0.004）. Frequent HCC examination is recommended for FIB-4 index ≥3 CH patients who obtain SVR after DAA treatment

都市部の妊娠期・出産後の女性の喫煙と食生活および生活習慣との関連

都市部に在住する乳児の母親を対象として,妊娠前後の喫煙の現状と食・生活習慣との関連を明らかにすることを目的に,無記名自記式アンケート調査を実施した。対象は,平成14年11月の1ヶ月間に,京都市内14ヶ所の保健所・支所にて,生後4ヶ月児健診を受診した991名の乳児の母親である。喫煙や食・生活習慣に関する調査紙を手渡し,回答が得られた466名(回収率47.0%)を解析対象とした。その結果,妊娠前喫煙率は28.5%で,年齢が若いほど喫煙率が高かった。妊娠を契機に74.4%が禁煙し,妊娠中喫煙率は7.3%であったが,出産後喫煙率は10.9%に増加していた。喫煙者は非喫煙者と比較して,食・生活習慣,授乳,喫煙に関する知識の欠如などの健康課題を有しており,小児期からの喫煙防止教育等,総合的な保健対策が望まれる

Relationship between the Plasma Levels of Catecholamines and Return of Spontaneous Circulation in Patients with Out-of-Hospital Cardiac Arrest

Purpose. The dynamic state of epinephrine (Ep) in the plasma of patients with out-of-hospital cardiac arrest (OHCA) remains unclear. The purpose of this study was to evaluate the relationship between the plasma levels of catecholamines (such as epinephrine (Ep), norepinephrine (Nep), and dopamine) and vasopressin (antidiuretic hormone (ADH)) and the acquisition of return of spontaneous circulation (ROSC) in OHCA patients. Methods. This was a prospective, observational clinical study. Patients with OHCA transferred to our hospital between July 2014 and July 2017 were enrolled. The levels of catecholamines and ADH in the plasma were measured using blood samples immediately obtained on arrival at our hospital and before the administration of Ep. Patients in whom Ep was already administered prior to obtaining blood samples were excluded. Patients were divided into two groups: with and without ROSC, that is, ROSC (+) and ROSC (−) groups, respectively. The plasma levels of these agents and the conditions of resuscitation were compared between the two groups. Results. A total of 96 patients with OHCA were analyzed. The ROSC (+) and ROSC (−) groups included 34 and 62 patients, respectively. There were no significant differences observed between the two groups in age, cause of cardiopulmonary arrest, and prehospital resuscitation time. The plasma levels of Ep and Nep were significantly lower in the ROSC (+) group than in the ROSC (−) group. However, there were no significant differences in the plasma levels of dopamine and ADH between the two groups. Conclusion. Increased levels of Ep in the plasma may not be associated with the acquisition of ROSC in patients with OHCA