Projekto metodo patirtis taikant nestandartinę jaunimo užimtumo formą „savanoryste grįstas turizmas“: Mariupolio viešųjų institucijų pavyzdys

The topic of the study is especially relevant because of the non-standard form of employment (NFE) ‘volunteering’ has acquired across the global. Similarly, NFE ‘voluntourism’ has recently been acquiring the form of a social movement and the development of mechanisms for public administration of these activities is far from complete. The aim of the article is to summarise the world experience of the project approach of public authorities to youth policy and the introduction of NFE of youth ‘volunteering’, as well as to analyse the experience of the micro-project approach of public authorities in Mariupol, Ukraine. The study is based on a comprehensive and systematic approach to public management of youth employment and uses methods to describe, summarise, analyse and synthesise the results of observations and experiments. The generalisation of the world experience of the project approach of public authorities to the introduction of non-standard forms of youth employment ‘volunteering’ and ‘voluntourism’ and the modern experience of its use is supported by UNESCO, which allows expanding its scope. It established that experience of the project approach of public authorities on creation of NFE ‘volunteering’ in the city of Mariupol, Ukraine, can be considered as innovative technology of information education. Thus, the project approach of public authorities with the use of NFE ‘volunteering’ and ‘voluntourism’ plays a specific useful role in professional and life situations of young people and allows them to perform social functions of professional, community and personal goals and competencies

T,2004, A new precursor for the immobilization of enzymes inside sol–gel-derived hybrid silica nanocompositescontaining polysaccharides

Abstract Tetrakis(2-hydroxyethyl) orthosilicate (THEOS) introduced by Hoffmann et al. (J. Phys. Chem. B., 106 (2002) 1528) was first used to prepare hybrid nanocomposites containing various polysaccharides and immobilize enzymes in these materials. Two different types of O-glycoside hydrolyses (EC3.2.1), 1 ! 3-h-D-glucanase L IV from marine mollusk Spisula sacchalinensis and a-D-galactosidase from marine bacterium Pseudoalteromonas sp. KMM 701, were taken for the immobilization. To reveal whether the polysaccharide inside the hybrid material influences the enzyme entrapment and functioning, negatively charged xanthan, cationic derivative of hydroxyethylcellulose and uncharged locust bean gum were examined. The mechanical properties of these nanocomposites were characterized by a dynamic rheology and their structure by a scanning electron microscopy. It was found that 1 ! 3-h-D-glucanase was usually immobilized without the loss of its activity, while the a-D-galactosidase activity in the immobilized state depended on the polysaccharide type of material. An important point is that the amount of immobilized enzymes was small, comparable to their content in the living cells. It was shown by the scanning electron microscopy that the hybrid nanocomposites are sufficiently porous that allows the enzymatic substrates and products to diffuse from an external aqueous solution to the enzymes, whereas protein molecules were immobilized firmly and not easily washed out of the silica matrix. A sharp increase of the enzyme lifetime (more than a hundred times) was observed after the immobilization. As established, the efficient entrapment of enzymes is caused by few advantages of new precursor over the currently used TEOS and TMOS: complete solubility of THEOS in water and the catalytic effect of polysaccharides on the sol -gel processes; (ii) the entrapment of enzymes can be performed at any pH which is suitable for their structural integrity and functionality; (iii) a gel can be prepared at reduced concentrations of THEOS (1 -2%) in the initial solution that excludes a notable heat release in the course of its hydrolysis.

Drosophila melanogaster Sperm under Simulated Microgravity and a Hypomagnetic Field: Motility and Cell Respiration

The role of the Earth’s gravitational and magnetic fields in the evolution and maintenance of normal processes of various animal species remains unclear. The aim of this work was to determine the effect of simulated microgravity and hypomagnetic conditions for 1, 3, and 6 h on the sperm motility of the fruit fly Drosophila melanogaster. In addition to the usual diet, the groups were administered oral essential phospholipids at a dosage of 500 mg/kg in medium. The speed of the sperm tails was determined by video recording and analysis of the obtained video files, protein content by western blotting, and cell respiration by polarography. The results indicated an increase in the speed of movement of the sperm tails after 6 h in simulated microgravity. The levels of proteins that form the axoneme of the sperm tail did not change, but cellular respiration was altered. A similar effect occurred with the administration of essential phospholipids. These results may be due to a change in the level of phosphorylation of motor proteins. Exposure to hypomagnetic conditions led to a decrease in motility after 6 h against a background of a decrease in the rate of cellular respiration due to complex I of the respiratory chain. This effect was not observed in the flies that received essential phospholipids. However, after 1 h under hypomagnetic conditions, the rate of cellular respiration also increased due to complex I, including that in the sperm of flies receiving essential phospholipids