Stem cell factor SALL4, a potential prognostic marker for myelodysplastic syndromes

Background: Myelodysplastic syndromes (MDS) are a group of heterogeneous diseases with variable clinical course. Predicting disease progression is difficult due to lack of specific molecular marker(s). SALL4 plays important roles in normal hematopoiesis and leukemogenesis. SALL4 transgenic mice develop MDS prior to acute myeloid leukemia (AML) transformation. However, the role of SALL4 in human MDS has not been extensively investigated. In this study, we evaluate the diagnostic/prognostic value of SALL4 in MDS by examining its expression levels in a cohort of MDS patients. Methods: Fifty-five newly diagnosed MDS, twenty MDS-AML, and sixteen post-treatment MDS patients were selected for our study along with ten healthy donors. Results: We demonstrated that SALL4 was over-expressed in MDS patients and proportionally increased in MDS patients with high grade/IPSS scores. This expression pattern was similar to that of Bmi-1, an important marker in predicting MDS/AML progression. In addition, the level of SALL4 was positively correlated with increased blast counts, high-risk keryotypes and increased significantly in MDS-AML transformation. Furthermore, higher level of SALL4 expression was associated with worse survival rates and SALL4 level decreased following effective therapy. Conclusions: To the best of our knowledge, this is the largest series and the first to report the expression pattern of SALL4 in detail in various subtypes of MDS in comparison to that of Bmi-1. We conclude that SALL4 is a potential molecular marker in predicting the prognosis of MDS

Development of Fatal Intestinal Inflammation in MyD88 Deficient Mice Co-infected with Helminth and Bacterial Enteropathogens

Infections with intestinal helminth and bacterial pathogens, such as enteropathogenic Escherichia coli, continue to be a major global health threat for children. To determine whether and how an intestinal helminth parasite, Heligomosomoides polygyrus, might impact the TLR signaling pathway during the response to a bacterial enteropathogen, MyD88 knockout and wild-type C57BL/6 mice were infected with H. polygyrus, the bacterial enteropathogen Citrobacter rodentium, or both. We found that MyD88 knockout mice co-infected with H. polygyrus and C. rodentium developed more severe intestinal inflammation and elevated mortality compared to the wild-type mice. The enhanced susceptibility to C. rodentium, intestinal injury and mortality of the co-infected MyD88 knockout mice were found to be associated with markedly reduced intestinal phagocyte recruitment, decreased expression of the chemoattractant KC, and a significant increase in bacterial translocation. Moreover, the increase in bacterial infection and disease severity were found to be correlated with a significant downregulation of antimicrobial peptide expression in the intestinal tissue in co-infected MyD88 knockout mice. Our results suggest that the MyD88 signaling pathway plays a critical role for host defense and survival during helminth and enteric bacterial co-infection

Epidemiology of potential drug- drug interactions in hospitalized patients with type 2 diabetes mellitus in China: a retrospective study

BackgroundCombination therapy was associated with an increased risk of drug- drug interactions (DDIs) in patients with type 2 diabetes mellitus (T2DM). The present study aimed to investigate the epidemiology of potential DDIs (pDDIs), including potential chemical drug-drug interactions (pCDIs) and potential herb-drug interactions (pHDIs), and classify the influencing factors of pDDIs in these patients.MethodsA retrospective study of the epidemiology of pDDIs among T2DM hospitalized patients older than 18 years and treated with at least two drugs during hospitalization was conducted over a 12-month period in 2019. PDDIs were identified with C (monitor therapy), D (consider therapy modification), and X (avoid combination) risk ratings. Binary logistic regression was used to analyze the risk factors of pDDIs.ResultsA total of 6796 pDDIs were identified from 737 T2DM hospitalized patients during hospitalization, with 0.87% classified as X risk rating, 13.39% as D risk rating. Additionally, 1753 pDDIs were identified after discharge, with 0.11% as X and 25.73% as D risk rating. The drug-drug association networks showed that the majority of pCDIs were associated with cardiovascular system drugs. Chlorphenamine-potassium chloride and danshen-warfarin were the most prevalent interacting pairs of pCDIs and pHDIs with X rating during hospitalization. Multivariate analysis indicated that the likelihood of developing over 4 pDDIs was significantly higher among T2DM patients who had received over 8 medications. The presence of pDDIs after discharge was strongly associated with the complications of T2DM and the number of discharge medications.ConclusionsT2DM patients were frequently exposed to pDDIs, including pCDIs and pHDIs, both during hospitalization and after discharge. Multi-drug combination was the primary risk factor for pDDIs. Strategies such as enhancing the monitoring and warning for pDDIs, increasing clinical pharmacological experience, as well as developing universally applicable clinical guidelines for pDDIs may be beneficial in reducing the incidence of potentially harmful drug-combinations

Sustainable Development of Employability of University Students Based on Participation in the Internship Promotion Programme of Zhejiang Province

In the context of severely restricted employment opportunities, undertaking an internship is an important way for students to enhance their employability and sustainable development. The aim of this study was to judge if the implementation of the Internship Promotion Programme (IPP) at universities in Zhejiang Province, China, could enhance graduates’ employability. We conducted surveys and interviews with 400 students and 100 business executives who participated in the IPP during the past 6 years. Based on the collected data and statistical analysis, the findings show that internships help students to improve their innovative thinking, interpersonal communication abilities (domestic and international), problem-solving skills, value creation and responsibility. Participants in the IPP were compared with students who did not take part. The results demonstrated that the IPP participants exhibited higher capabilities to perform important workplace tasks and that as a result, they became more attractive to employers. The analysis, therefore, demonstrates a positive correlation between the internship experience and employability. As a result, it can be concluded that the IPP improves students’ employability and is beneficial to enhancing the quality of higher education, and hence the sustainable development of students

Sustainable Development of Employability of University Students Based on Participation in the Internship Promotion Programme of Zhejiang Province

In the context of severely restricted employment opportunities, undertaking an internship is an important way for students to enhance their employability and sustainable development. The aim of this study was to judge if the implementation of the Internship Promotion Programme (IPP) at universities in Zhejiang Province, China, could enhance graduates’ employability. We conducted surveys and interviews with 400 students and 100 business executives who participated in the IPP during the past 6 years. Based on the collected data and statistical analysis, the findings show that internships help students to improve their innovative thinking, interpersonal communication abilities (domestic and international), problem-solving skills, value creation and responsibility. Participants in the IPP were compared with students who did not take part. The results demonstrated that the IPP participants exhibited higher capabilities to perform important workplace tasks and that as a result, they became more attractive to employers. The analysis, therefore, demonstrates a positive correlation between the internship experience and employability. As a result, it can be concluded that the IPP improves students’ employability and is beneficial to enhancing the quality of higher education, and hence the sustainable development of students

Microfluidics-derived microcarrier systems for oral delivery

Oral delivery is with high patient compliance but low bioavailability and controllability. The emerging microfluidics-derived microcarriers have sprung up to relieve the hurdles. Herein, a review on the recent progress in applying microfluidics-derived microcarriers for oral delivery is presented. First, we briefly introduce the microfluidic fabrication of microcarriers based on laminar flow and droplets. Then, we focus on the applications of microfluidics-derived microcarriers for oral delivery in stomach, small intestine, and colon. Last but not least, we discuss the challenges and outlooks of the microfluidics-derived microparticles for oral delivery in the end. This review is anticipated to help readers to follow up the research frontiers in this field, and guide the future development direction of microfluidics-derived microcarriers

Radiative and non-radiative exciton recombination rate constants in ZnSe clusters

Understanding the origin of photoluminescence intermittency and its correlation with microstructure is crucial for the design and preparation of quantum dots (QDs) with high fluorescence quantum yield. ZnSe clusters provide a typical model for studying the effect of their size, geometrical and electronic structures on their radiative and non-radiative process of II-VI QDs. The rate constants of radiative and non-radiative processes, kr and knr , of the (ZnSe) n clusters were computed by using first-principles calculations, Einstein spontaneous radiation theory and Fermi’s golden rule. The kr and knr variations with cluster size were analyzed in term of a number of quantities. Emission energy and reorganization energy were identified to play dominant roles in the determination of kr and knr for the studied clusters. Furthermore, a correlation between these two quantities and the geometric rigidity of the ZnSe clusters was revealed. The clusters with greater geometric rigidity tend to possess larger emission energy, and smaller reorganization energy. While radiative and non-radiative electron–hole recombination rates of the ZnSe clusters vary in a complicated way because of their diverse structures and prominent quantum size effect, our study highlights the correlation between recombination rate and cluster structure, which would be helpful for the design of QD materials with high fluorescence quantum yields

Quasi-Solid-State Lithium-Sulfur Batteries Assembled by Composite Polymer Electrolyte and Nitrogen Doped Porous Carbon Fiber Composite Cathode

Solid-state lithium sulfur batteries are becoming a breakthrough technology for energy storage systems due to their low cost of sulfur, high energy density and high level of safety. However, its commercial application has been limited by the poor ionic conductivity and sulfur shuttle effect. In this paper, a nitrogen-doped porous carbon fiber (NPCNF) active material was prepared by template method as a sulfur-host of the positive sulfur electrode. The morphology was nano fiber-like and enabled high sulfur content (62.9 wt%). A solid electrolyte membrane (PVDF/LiClO4/LATP) containing polyvinylidene fluoride (PVDF) and lithium aluminum titanium phosphate (Li1.3Al0.3Ti1.7(PO4)3) was prepared by pouring and the thermosetting method. The ionic conductivity of PVDF/LiClO4/LATP was 8.07 × 10−5 S cm−1 at 25 °C. The assembled battery showed good electrochemical performance. At 25 °C and 0.5 C, the first discharge specific capacity was 620.52 mAh g−1. After 500 cycles, the capacity decay rate of each cycle was only 0.139%. The synergistic effect between the composite solid electrolyte and the nitrogen-doped porous carbon fiber composite sulfur anode studied in this paper may reveal new approaches for improving the cycling performance of a solid-state lithium-sulfur battery