Downregulation of Brassica napus MYB69 (BnMYB69) increases biomass growth and disease susceptibility via remodeling phytohormone, chlorophyll, shikimate and lignin levels

MYB transcription factors are major actors regulating plant development and adaptability. Brassica napus is a staple oil crop and is hampered by lodging and diseases. Here, four B. napus MYB69 (BnMYB69s) genes were cloned and functionally characterized. They were dominantly expressed in stems during lignification. BnMYB69 RNA interference (BnMYB69i) plants showed considerable changes in morphology, anatomy, metabolism and gene expression. Stem diameter, leaves, roots and total biomass were distinctly larger, but plant height was significantly reduced. Contents of lignin, cellulose and protopectin in stems were significantly reduced, accompanied with decrease in bending resistance and Sclerotinia sclerotiorum resistance. Anatomical detection observed perturbation in vascular and fiber differentiation in stems, but promotion in parenchyma growth, accompanied with changes in cell size and cell number. In shoots, contents of IAA, shikimates and proanthocyanidin were reduced, while contents of ABA, BL and leaf chlorophyll were increased. qRT-PCR revealed changes in multiple pathways of primary and secondary metabolisms. IAA treatment could recover many phenotypes and metabolisms of BnMYB69i plants. However, roots showed trends opposite to shoots in most cases, and BnMYB69i phenotypes were light-sensitive. Conclusively, BnMYB69s might be light-regulated positive regulators of shikimates-related metabolisms, and exert profound influences on various internal and external plant traits

Assessing perceived participation among older adults total hip arthroplasty and total knee arthroplasty patients six months post-surgery: a cross-sectional study

AimsThis research sought to assess the perceived levels of participation and autonomy in senior patients who had received total hip arthroplasty (THA) or total knee arthroplasty (TKA) in Hangzhou, China. Furthermore, the study aimed to identify the factors linked to these outcomes.Study designThis investigation will utilize a cross-sectional study design to assess perceived participation and autonomy among older adults total hip arthroplasty (THA) and total knee arthroplasty (TKA) patients. The research was conducted in Hangzhou, China, at a tertiary hospital.MethodsConvenient sampling was utilized to select 139 patients who underwent THA or TKA between March 2022 and March 2023 and met the inclusion criteria at a tertiary hospital in Hangzhou. The Impact on Participation and Autonomy Questionnaire, Hip/Knee Injury and Osteoarthritis Outcome Score (HOOS/KOOS), 5-Item Geriatric Depression Scale, Multidimensional Scale of Perceived Social Support, and Elders Health Empowerment Scale were used to assess perceived participation, hip/knee-related symptoms and functional restrictions, depression symptoms, social support, and health empowerment.ResultsThe mean score for perceived participation and autonomy was 22.554 (SD: 13.042). The mean scores for participation in indoor autonomy, outdoor autonomy, family roles, and social relations were 0.654 (SD: 0.608), 1.324 (SD: 0.792), 1.053 (SD: 0.657), and 0.664 (SD: 0.542), respectively. Negative correlations were observed between perceived participation/autonomy scores and HOOS/KOOS, social support, and health empowerment scores. Conversely, a positive correlation was found between perceived participation/autonomy scores and depression scores. The detrimental effect of HOOS/KOOS, social support, and health empowerment scores on perceived participation and autonomy was notable, while the impact of depressive symptoms was comparatively minor.ConclusionOlder Chinese patients, at first six months post THA/TKA surgery, reported higher levels of perceived participation compared to individuals with other conditions, such as stroke patients. Functional limitations resulting from hip/knee-related symptoms, as well as social support and health empowerment, emerged as significant influencing factors for perceived participation and autonomy. This research enhances our comprehension of the elements influencing perceived participation among older adults individuals who have undergone THA/TKA procedures

Research on New Whitening and Water-Saving Technology Based on Industrial Equipment

Energy conservation and consumption reduction have always been the goals pursued by the power industry. Based on these goals, this study explored a new type of whitening and water-saving technology for industrial equipment. Unlike existing direct heating and condensation heating technologies, the main innovation of this work lies in not changing the saturated wet flue gas before it is discharged into the atmospheric environment. A series of experiments were conducted on electrode plates with different wind speeds, supersaturation levels, and porosities using three principles, namely droplet electrostatic adsorption, ionic-wind-enhanced condensation, and droplet dipole deflection, through the construction of a parameter-adjustable and -controllable enthalpy and humidity chamber and a pilot development platform with a wind volume of 10,000 m3/h. In addition, the collection efficiency was calculated using thermodynamic laws. The results showed that, under the working conditions of white mist supersaturation of 5.77 g/kg, a hole opening rate of 70%, and a wind speed of 3 m/s, the water collection efficiency was the highest—close to 60%—verifying the feasibility of this technology. This technology not only eliminates white smoke but also saves water resources and has certain economic benefits, providing support for the development of industrial equipment for smoke removal in the future

Coal mining collapse analysis of total caving method based on FLAC3D and UAV aerial surveying

Objective Total caving coal mining in the upper reaches of the Yellow River in Inner Mongolia has resulted in large areas of subsidence, causing frequent ecological and environmental problems on the surface. Methods For the study of the surface crack development rule of the goaf and collapse stress and the displacement field evolution characteristics, for a string of the Chuancaogedan Coal Mine 6102 working face as the research object. The distribution range and rule of surface cracks were found by UAV aerial survey, a FLAC3D numerical model was constructed to analyze the variation of stress and displacement of surrounding rock in goaf. Results The results of the analysis were combined with the results of UAV mutual authentication. The results show that (1) The collapse is mainly in the form of ground fissures, which are mainly distributed in the northwest and midwest of the mine. There are two types of ground fractures in the working face. One type of arc-shaped stepped fracture group is distributed in parallel and appears at intervals of 5-20 m. Most of them form staggered platforms with a step height of 15-130 cm, and the fractures develop in the direction of the vertical working face advance at a deviation angle of 3°-5°. For the other kind of linear edge crack belts, exhibiting severe tensile damage, were observed along the working face peripheral, belt development can occur, a few can be spread in the inside of the working face, and the distance from the outermost crack to the working face is 38.53 m. (2) During the excavation of the underground coal seam, an obvious saddle-shaped tensile stress concentration area appears in the roof of the goaf, and with the advance of excavation, the tensile stress in the surface concentration area first increases and then decreases, with a maximum value of 0.181 MPa. (3) Complete collapse occurred at the top of the goaf, with the maximum surface vertical displacement of approximately 5.5 m located at the middle of the goaf. The maximum surface horizontal displacement was located above the coal pillar of the goaf, with a maximum value of 1.93 m. (4) Numerical simulation results regarding settlement and crack angles were found to align closely with the UAV survey data. Conclusion The research results can provide a reference for the solution of ecological environmental problems caused by coal seam mining

Micromorphology and Mechanical and Dielectric Properties of Bismaleimide Composite Modified by Multiwalled Carbon Nanotubes and Polyethersulfone

Multiwalled carbon nanotubes (MWCNTs) were modified by oxidizing agent to obtain O-MWCNTs, and the surface of it was coated with active group. 4,4′-Diaminodiphenylmethane bismaleimide (MBMI) was used as matrix, 3,3′-diallyl bisphenol A (BBA) and bisphenol-A diallyl ether (BBE) were used as reactive diluent, polyethersulfone (PES) as toughening agent, and O-MWCNTs as modifier; OMWCNT/PES-MBAE composite was prepared through in situ sol-gel method. The effect of PES and OMWCNTs on the mechanical and dielectric properties of composite was analyzed, and the microstructure was examined by transmission electron microscope (TEM) and scanning electron microscope (SEM). The mechanism of composite toughened by PES and OMWCNTs was observed and analyzed. The results showed that Diels-Alder reaction between MBMI and allyl compounds occurred completely and unsaturated double bond disappeared. O-MWCNTs and PES resin dispersed smoothly in polymer matrix and were used as reinforcement, and PES resin and O-MWCNTs could synergistically improve the properties of the composite and exhibited a typical ductile fracture. The impact and bending strengths were 16.09 kJ/m2 and 153.57 MPa, which were 74.32% and 53.08% higher than those of the MBAE matrix, respectively, and the dielectric constant and the dielectric loss were 3.76 (100 Hz) and 2.79 × 10−3 (100 Hz), when the content of PES was 2 wt% and O-MWCNTs was 0.02 wt%. The outstanding properties of the material made it play an important role in high-performance insulating material applications

Rainfall Induced Shallow Landslide Temporal Probability Modelling and Early Warning Research in Mountains Areas: A Case Study of Qin-Ba Mountains, Western China

The rainfall-induced landslide early warning model (LEWM) is an important means to mitigate property loss and casualties, but the conventional discriminant matrix-based LEWM (DLEWM) leaves room for subjectivity and limits warning accuracy. Additionally, it is important to employ appropriate indicators to evaluate warning model performance. In this study, a new method for calculating the spatiotemporal probability of rainfall-induced landslides based on a Bayesian approach is proposed, and a probabilistic-based LEWM (PLEWM) at the regional scale is developed. The method involves four steps: landslide spatial probability modeling, landslide temporal probability modeling, coupling of spatial and temporal probability models, and the conversion method from the spatiotemporal probability index to warning levels. Each step follows the law of probability and is tested with real data. At the same time, we propose the idea of using economic indicators to evaluate the performance of the multilevel LEWM and reflect its significant and unique aspects. The proposed PLEWM and the conventional DLEWM are used to conduct simulate warnings for the study area day-by-day in the rainy season (July-September) from 2016 to 2020. The results show that the areas of the 2nd-, 3rd-, and 4th-level warning zones issued by the PLEWM account for 60.23%, 45.99%, and 43.98% of those of the DLEWM, respectively. The investment in issuing warning information and the losses caused by landslides account for 54.54% and 59.06% of those of the DLEWM, respectively. Moreover, under extreme rainfall conditions, the correct warning rate of the PLEWM is much higher than that of the DLEWM

Correlating genomic copy number alterations with clinicopathologic findings in 75 cases of hepatocellular carcinoma

Abstract Background Oligonucleotide array comparative genomic hybridization (aCGH) analysis has been used for detecting somatic copy number alterations (CNAs) in various types of tumors. This study aimed to assess the clinical utility of aCGH for cases of hepatocellular carcinoma (HCC) and to evaluate the correlation between CNAs and clinicopathologic findings. Methods aCGH was performed on 75 HCC cases with paired DNA samples from tumor and adjacent nontumor tissues. Survival outcomes from these cases were analyzed based on Barcelona-Clinic Liver Cancer Stage (BCLC), Edmondson-Steiner grade (E-S), and recurrence status. Correlation of CNAs with clinicopathologic findings was analyzed by Wilcoxon rank test and clustering vs. K means. Results The survival outcomes indicated that BCLC stages and recurrence status could be predictors and E-S grades could be a modifier for HCC. The most common CNAs involved gains of 1q and 8q and a loss of 16q (50%), losses of 4q and 17p and a gain of 5p (40%), and losses of 8p and 13q (30%). Analyses of genomic profiles and clusters identified that losses of 4q13.2q35.2 and 10q22.3q26.13 seen in cases of stage A, grade III and nonrecurrence were likely correlated with good survival, while loss of 1p36.31p22.1 and gains of 2q11.2q21.2 and 20p13p11.1 seen in cases of stage C, grade III and recurrence were possibly correlated with worst prognosis. Conclusions These results indicated that aCGH analysis could be used to detect recurrent CNAs and involved key genes and pathways in patients with HCC. Further analysis on a large case series to validate the correlation of CNAs with clinicopathologic findings of HCC could provide information to interpret CNAs and predict prognosis

Supplementation of

To investigate the therapeutic effect of polyphenol from the fruits of Rhodomyrtus tomentosa (RTFP) on experimental colitis induced by dextran sulphate sodium(DSS) in mice. A total of 24 Babl/c mice were randomly divided into normal control group, colitis model group and DSS+RTFP intervention group, respectively. The intestinal disease activity index (DAI), pathological histology of colon were investigated, and the changes of gut microbiota in mice were evaluated by 16S rRNA sequencing. Compared with the DSS-induced colitis group, RTFP showed intestinal anti-inflammatory effects, the DAI score was significantly decreased, RTFP improved colitis induced weight loss, fecal imformation and blood in the stool. RTFP relieved the phenomenon of shortening colon length, shortening intestinal wall, and splenomegaly. RTFP intervention inhibited the increase of bacteroides abundance caused by DSS, and remodeled the diversity of gut microbiota. Taken together, RTFP could effectively intervene in experimental colitis induced by DSS in mice, which may be related to the modulating gut microbiota and intestinal cell immune balance