Xiaochaihu Decoction Treatment of Chicken Colibacillosis by Improving Pulmonary Inflammation and Systemic Inflammation

Chicken colibacillosis—the most common disease of poultry, is caused mainly by avian pathogenic Escherichia coli (APEC). It has a major impact on the poultry industry worldwide. The present study was conducted to investigate the therapeutic effects of Xiaochaihu Decoction (XCHD) supplementation on clinical manifestation, organ index, bacterial load in organ and inflammatory mediators in a chicken model challenged with APEC. The results showed that all doses of XCHD significantly elevated the survival rate of infected chickens. XCHD improved the clinical signs of infected chickens, reduced the organ index, reduced the bacterial load of organs, and inhibited the secretion of serum and pulmonary inflammatory factors IL-1β, IL-6 and TNF- α. Taken together, this study demonstrates that XCHD had protective effects on APEC-infected chickens. Its mechanism includes anti-inflammatory and antibacterial effects. These findings may contribute to the further study of the mechanism of the formula and the prevention or treatment of colibacillosis in poultry. The significance of this study is that it provides a certain theoretical basis for the replacement of antibiotics by XCHD

Ocular surface involvement and histopathologic changes in the acute stage of Stevens-Johnson syndrome and toxic epidermal necrolysis: a cross-sectional study

Abstract Background Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are rare and extremely serious drug-induced dermatological disorders. The ocular surface condition at the early stage has been little studied and should contribute to novel perspectives in early and effective topical therapy of these diseases. The objectives of the study were to evaluate the acute phase of ocular surface involvement and histopathologic changes in patients with acute SJS/TEN. Methods Ten patients with acute phase of SJS/TEN onset and eleven age- and sex-matched healthy volunteers were recruited. Ocular surface symptoms and signs, conjunctival impression cytology, and tear multi-cytokine were assessed. Results Ocular surface objective signs were normal at the acute stage of SJS/TEN, while most patients have abnormal ocular surface subjective symptoms and meibomian gland secretion. Conjunctival impression cytology showed a significant decrease in goblet cell density and severe ocular surface squamous metaplasia in acute SJS/TEN patients. Tear multi-cytokine analysis showed all 21 pro- and anti-inflammatory cytokines all sharply elevated. Goblet cell density was significantly negatively correlated with tear C-X3-C motif chemokine ligand 1 (CX3CL1) and interleukin 13. Conclusions Severe pathologic squamous metaplasia and inflammation onset in the ocular surface at the acute stage of the SJS/TEN, even if the ocular surface condition seemed basically normal with adequate systemic immunosuppressant and general supportive treatment. Early topical anti-inflammatory therapy should be carried out actively

Coronatine Enhances Chilling Tolerance of Tomato Plants by Inducing Chilling-Related Epigenetic Adaptations and Transcriptional Reprogramming

Low temperature is an important environmental factor limiting the widespread planting of tropical and subtropical crops. The application of plant regulator coronatine, which is an analog of Jasmonic acid (JA), is an effective approach to enhancing crop’s resistance to chilling stress and other abiotic stresses. However, the function and mechanism of coronatine in promoting chilling resistance of tomato is unknown. In this study, coronatine treatment was demonstrated to significantly increase tomato chilling tolerance. Coronatine increases H3K4me3 modifications to make greater chromatin accessibility in multiple chilling-activated genes. Corresponding to that, the expression of CBFs, other chilling-responsive transcription factor (TF) genes, and JA-responsive genes is significantly induced by coronatine to trigger an extensive transcriptional reprogramming, thus resulting in a comprehensive chilling adaptation. These results indicate that coronatine enhances the chilling tolerance of tomato plants by inducing epigenetic adaptations and transcriptional reprogramming

Melatonin Mediates the Regulation of Morphological and Anatomical Traits in <i>Carex leucochlora</i> under Continuous Salt Stress

Soil salinity is one of the most critical factors limiting plant growth and development. Carex leucochlora is an important turfgrass species with a wide distribution in northern China that is highly sensitive to salt stress, which impairs its development. Recently, melatonin has emerged as a nontoxic biomolecule that regulates growth and enhances salt tolerance in plants. In this study, the mechanism of melatonin’s regulation of plant growth and anatomical characteristics in C. leucochlora seedlings under continuous salt stress was explored. Our results indicated that salt stress strongly suppressed plant growth and leaf cell activity, inhibited root morphology and root activity, and negatively affected leaf and root anatomic structures in the seedlings. Conversely, melatonin (150 μmol L−1) pretreatment improved the detrimental effect of salt stress by restoring the morphology of the leaf, alleviating damage to the cell membrane, improving root activity, and altering the root architecture and plant growth attributes. Moreover, after 12 days of salt stress, anatomical observations of the leaf showed that the thickness of the leaf blade, vascular bundle area of the leaf main vein, vesicular cell area, thickness of the upper epidermis, and thickness of the lower epidermis were increased by 30.55, 15.63, 12.60, 16.76 and 27.53%, respectively, with melatonin under salinity. Melatonin treatment also showed an increase of 5.91, 7.59, 15.57, and 20.51% in epidermal thickness, vascular cylinder diameter, xylem vessel diameter, and pith cell diameter, respectively, compared with salt stress after 12 days. These results suggest that melatonin alleviated salt stress through augmenting seedling growth, leaf cell activity, and root characteristics, maintained the stability of anatomic traits to maintain chloroplast cell homeostasis, and also protected the vascular tissues to promote the radial transport of water and ions in the C. leucochlora seedlings. These modifications induced by the exogenous application of melatonin may help C. leucochlora to acclimate successfully to saline soils

Exogenous Melatonin Improves Seed Germination of Wheat (<i>Triticum aestivum</i> L.) under Salt Stress

Melatonin (MT) can effectively reduce oxidative damage induced by abiotic stresses such as salt in plants. However, the effects of MT on physiological responses and molecular regulation during wheat germination remains largely elusive. In this study, the response of wheat seeds to MT under salt stress during germination was investigated at physiological and transcriptome levels. Our results revealed that application of MT significantly reduced the negative influence of salt stress on wheat seed germination. The oxidative load was reduced by inducing high activities of antioxidant enzymes. In parallel, the content of gibberellin A3 (GA3) and jasmonic acid (JA) increased in MT-treated seedling. RNA-seq analysis demonstrated that MT alters oxidoreductase activity and phytohormone-dependent signal transduction pathways under salt stress. Weighted correlation network analysis (WGCNA) revealed that MT participates in enhanced energy metabolism and protected seeds via maintained cell morphology under salt stress during wheat seed germination. Our findings provide a conceptual basis of the MT-mediated regulatory mechanism in plant adaptation to salt stress, and identify the potential candidate genes for salt-tolerant wheat molecular breeding

Melatonin-Mediated Enhancement of Photosynthetic Capacity and Photoprotection Improves Salt Tolerance in Wheat

The role of melatonin in plant growth and response to environmental stress has been widely demonstrated. However, the physiological and molecular regulation of salt tolerance in wheat seedlings by melatonin remains unclear. In this study, we investigated changes in phenotype, physiology, photosynthetic parameters, and transcript levels in wheat seedlings to reveal the role of melatonin in the regulation of salt tolerance in wheat. The results indicate that the application of exogenous melatonin significantly alleviates growth inhibition, reactive oxygen species accumulation, and membrane oxidative damage induced by salt stress in wheat. Additionally, exogenous melatonin increased antioxidant enzyme activity and regulated photosynthetic gas exchange. Transcriptomic data showed a significant up-regulation of genes encoding light-harvesting chlorophyll protein complex proteins in photosynthesis and genes related to chlorophyll and carotenoid biosynthesis under the influence of melatonin. These results suggest that exogenous melatonin improves salt tolerance in wheat seedlings by enhancing the antioxidant, photoprotective, and photosynthesis activities

Soil Moisture Retrieval from Multi-GNSS Reflectometry on FY-3E GNOS-II by Land Cover Classification

The reflected GNSS signals at the L-band is significantly advantageous in soil moisture monitoring as they are sensitive to the dielectric properties determined by the volumetric water content of topsoil, and they can penetrate vegetation, except in very dense forests. The Global Navigation satellite system Occultation Sounder (GNOS-II) with a reflectometry technique onboard the Fengyun-3E (FY-3E) satellite, launched on 5 July 2021, is the first mission that can receive reflected Global Navigation Satellite System (GNSS) signals from GPS, BeiDou and Galileo systems. This paper presents the soil moisture retrieval results from the FY-3E GNOS-II mission using 16 months of data. In this study, the reflectivity observations from different GNSS systems were firstly intercalibrated with some differences analyzed. Observations were also corrected by considering vegetation attenuation for 16 different land cover classifications. Finally, an empirical model was constructed for volumetric soil moisture (VSM) estimation, where the reflectivity of GNOS-II was linearly related to the SMAP reference soil moisture for each 36 km ease grid. The overall root-mean-square error of the retrieved soil moisture is 0.049 compared with the SMAP product, and 0.054 compared with the in situ data. The results of the three GNSS systems show similar levels of accuracy. This paper, for the first time, demonstrates the feasibility of global soil moisture retrieval using multiple GNSS signals