ヒトCYP4B1 mRNA発現の臓器差

The cytochrome P450 enzymes (GYP) play important roles in the metabolism of many therapeutic agents and environmental compounds. Differences in the activities of these enzymes are thought to be responsible for individual variability in drug response and/or toxicity. Among the GYP enzymes, the isoform CYP4B1 is of particular interest and involved in the metabolism of several protoxins such as 2-aminoanthracene and 2-aminofluorene, all of which are bladder carcinogens. The present study compared the relative expression pattern of CYP4B1 in cDNA panels of human tissues. We developed an assay for CYP4B1 mRNA by performing real-time RT-PCR. Using this method, CYP4B1 mRNA levels were analyzed in human tissues. CYP4B1 mRNA was selectively expressed in lung, bladder, and prostate. These findings suggest that a high expression of CYP4B1 increases the risk of tumor by activation of carcinogenic compounds