Additional file 2: Figure S2. of Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination

Expression of ABA metabolism genes in response to sinapic acid. a Quantitative real-time RT-PCR (qRT-PCR) to examine the expression of ABA catabolism genes (CYP707A1, CYP707A2, CYP707A3, and CYP707A4) with sinapic acid. b qRT-PCR to examine the expression of de novo ABA biosynthesis genes (BG1 and BG2) with sinapic acid. The seeds were incubated with 0.5 mM sinapic acid or dimethyl sulfoxide (DMSO) for 36 h at 4 °C in the dark and then germinated on MS agar medium for 1 d. Total RNA was isolated from the treated and mock-treated seeds; Actin2 primers were used as an internal control. (PPTM 107 kb

Additional file 4: Figure S4. of Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination

Germination of aba2-1 and abi3-1 seeds in the presence of 400 mg l−1 choline chloride. (PPTM 69 kb

Additional file 3: Figure S3. of Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination

Quantification of sinapoylcholine released from wild-type and fah1–1 seeds pretreated with 0.5 mM sinapic acid. After 2 d of treatment with 0.5 mM sinapic acid, sinapic acid esters were extracted from seeds and sinapoylcholine was quantified by HPLC; DW, dry weight. (PPTM 58 kb

Additional file 5: Table S1. of Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination

List of primers used in this study. (DOCX 14 kb

MOESM2 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 2. Pictorial representation of the Agrobacterium-mediated transient transformation of G. hirsutum

MOESM10 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 10. GUS staining of plants transiently expressing GhGPX1 promoter constructs using Agrobacterium EHA105-mediated transient transformation at different times after inoculation

MOESM8 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 8. Transformation efficiency

MOESM6 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 6. GUS staining of transiently transformed cotton seedlings

MOESM5 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 5. Transient transformation of cotton seedlings with GhGPX1pro-GUS under vacuum infiltration with or without Agrobacterium in the transformation solution

MOESM9 of A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Additional file 9. Quantification of relative expression levels for GhGPX1 and GhWRKY40 in Agrobacterium-inoculated G. hirsutum plants using two different endogenous reference genes