Evaluation of Analgesic and Anti-Inflammatory Activities of Water Extract of Galla Chinensis In Vivo

Aim. Pain and inflammation are associated with many diseases in humans and animals. Galla Chinensis, a traditional Chinese medicine, has a variety of pharmacological properties. The purpose of this study was to evaluate analgesic and anti-inflammatory activities of Galla Chinensis through different animal models. Method. The analgesic activities were evaluated by hot-plate and writhing tests. The anti-inflammatory effects were assessed by ear edema, capillary permeability, and paw edema tests. The contents of cytokines (NO, iNOS, PGE2, and IL-10) in serum of rats in paw edema test were inspected by ELISA assays. Results. In the hot-plate test, Galla Chinensis could significantly extend pain threshold when compared to control group. The inhibitory rates of writhes ranged from 36.62% to 68.57% in Galla Chinensis-treated mice. Treatment with Galla Chinensis (1 and 0.5 g/kg) could significantly inhibit ear edema (47.45 and 36.91%, resp.; P < 0.01). Galla Chinensis (1 g/kg) had significant (P < 0.05) anti-inflammatory activity in capillary permeability test (29.04%). In carrageenan-induced edema test, the inhibitory rates were 43.71% and 44.07% (P < 0.01) at 1 h and 2 h after administration of Galla Chinensis (1 g/kg), respectively, and the levels of proinflammatory cytokines were significantly reduced. Conclusion. These results suggest that Galla Chinensis has analgesic and anti-inflammatory effects, which may be a candidate drug for the treatment of inflammation and pain

In Vivo

Aim. Dermatophytosis is one of the main fungal diseases in humans and animals all over the world. Galla chinensis, a traditional medicine, has various pharmacological effects. The goal of this study was to evaluate the treatment effect of Galla chinensis solution (GCS) on dermatophytosis-infected dogs (Microsporum canis, Microsporum gypseum, and Trichophyton mentagrophytes, resp.). Methods. The treatment effects of GCS were evaluated by mycological cure rates and clinical score comprised of three indices, including inflammation, hair loss, and lesion scale. Results. The results showed that, in the three models of dermatophytosis, GCS significantly (P<0.05) improved skin lesions and fungal eradication. GCS (10% and 5%) had higher efficacy compared to the positive control (Tujingpi Tincture). The fungal eradication efficacy exceeds 85% after treatment with GCS (10%, 5%, and 2.5%) on day 14. Conclusion. The GCS has antidermatophytosis effect in dogs, which may be a candidate drug for the treatment of dermatophytosis

A comparison of volatile fractions obtained from Lonicera macranthoides via different extraction processes: ultrasound, microwave, Soxhlet extraction, hydrodistillation, and cold maceration

Background: Hydrodistillation has been traditionally used to extract volatile fraction in traditional Chinese medicine. However, with the development of Soxhlet extraction (SE), microwave (MW), ultrasound (US), and cold maceration (CM), hydrodistillation (HD) is being replaced to meet some practical requirements. In this study, we investigated the effect of the five methods on the volatile fraction extract of Lonicera macranthoides. Methods: Volatile fraction from the flower buds of Lonicera macranthoides was obtained by using different extraction methods, HD, SE, MW, US, and CM. The compositions of volatile fraction were analyzed by gas chromatography–mass spectrometric and further compared among extraction methods. Results: Extracts obtained by the five methods reveal the qualitative and quantitative diversity in their compositions, especially for the low-content compositions. According to the results, SE shows the great value in the research where the high molecular-mass compound is of primary interest, and MW offers a way for the isolation of specific compound like octadecadienoic acid and hexadecanoic acid. HD, US, and CM have the advantage over SE and MW for the integrity of the constituents, whereas the phenomenon of compound degradation seems not so serious in solvent extraction methods such as US or CM as HD. Additionally, US and CM show superiority over time or material saving and diversity of the constituent. Conclusion: HD is still the best choice for the pure volatile fraction without organic solvent pollution. However, when it comes to some specifically actual demands, it can be replaced by the four methods for the volatile fraction extraction process, especially for production of certain compound groups

Progress on direct assembly approach for in situ fabrication of electrodes of reversible solid oxide cells

Reversible solid oxide cells (SOCs) are very efficient and clean for storage and regeneration of renewable electrical energy by switching between electrolysis and fuel cell modes. One of the most critical factors governing the efficiency and durability of SOCs technology is the stability of the interface between oxygen electrode and electrolyte, which is conventionally formed by sintering at a high temperature of ~1000–1250 ​°C, and which suffers from delamination problem, particularly for reversibly operated SOCs. On the other hand, our recent studies have shown that the electrode/electrolyte interface can be in situ formed by a direct assembly approach under the electrochemical polarization conditions at 800 ​°C and lower. The direct assembly approach provides opportunities for significantly simplifying the cell fabrication procedures without the doped ceria barrier layer, enabling the utilization of a variety of high-performance oxygen electrode materials on barrier layer–free yttria-stabilized zirconia (YSZ) electrolyte. Most importantly, the in situ polarization induced interface shows a promising potential as highly active and durable interface for reversible SOCs. The objective of this progress report is to take an overview of the origin and research progress of in situ fabrication of oxygen electrodes based on the direct assembly approach. The prospect of direct assembly approach in the development of effective SOCs and in the fundamental studies of electrode/electrolyte interface reactions is discussed

Oracle-free repair synthesis for floating-point programs

The floating-point representation provides widely-used data types (such as “float” and “double”) for modern numerical software. Numerical errors are inherent due to floating-point’s approximate nature, and pose an important, well-known challenge. It is nontrivial to fix/repair numerical code to reduce numerical errors — it requires either numerical expertise (for manual fixing) or high-precision oracles (for automatic repair); both are difficult requirements. To tackle this challenge, this paper introduces a principled dynamic approach that is fully automated and oracle-free for effectively repairing floating-point errors. The key of our approach is the novel notion of micro-structure that characterizes structural patterns of floating-point errors. We leverage micro-structures’ statistical information on floating-point errors to effectively guide repair synthesis and validation. Compared with existing state-of-the-art repair approaches, our work is fully automatic and has the distinctive benefit of not relying on the difficult to obtain high-precision oracles. Evaluation results on 36 commonly-used numerical programs show that our approach is highly efficient and effective: (1) it is able to synthesize repairs instantaneously, and (2) versus the original programs, the repaired programs have orders of magnitude smaller floating-point errors, while having faster runtime performance.ISSN:2475-142

Protective effect of isoflavones and triterpenoid saponins from pueraria lobata on liver diseases: A review

In recent years, with the improvement of people's living standard and the change of diet structure, liver disease and its related complications have become a significant public health problem globally. Pueraria lobata (Pueraria montana var. lobata (Willd.) Sanjappa &amp; Pradeep) belongs to the genus Pueraria, which is widely planted and used as medicine and food in Asia with a long history. A variety of natural active products, including puerarin, daidzein, formononetin, genistein, and soyasaponin, have been isolated and identified from pueraria lobata. A large number of studies have shown that various natural active products of pueraria lobata can play a protective role in different types of liver diseases by regulating oxidative stress, inflammatory response, lipid metabolism, etc. In this review, we focused on the protective effects of isoflavones and triterpenoid saponins from pueraria lobata on the liver through different targeted therapeutic mechanisms. What's more, we summarized their therapeutic potential for different types of liver diseases to provide evidence for their clinical application

Translational potential of ginsenoside Rb1 in managing progression of osteoarthritis

Background: Osteoarthritis (OA) is the most common degenerative joint disorder. Inflammatory cytokine plays an important role in OA progression. Previous studies have demonstrated that ginsenoside Rb1 would prevent inflammation and apoptosis in chondrocytes. However, we have not found any animal study reporting that Rb1 attenuates the severity of OA. Objective: In this study, we used a rat anterior cruciate ligament transaction plus medial meniscus resection (ACLT + MMx) model of OA and a cell model, to investigate whether administration of ginsenoside Rb1 may attenuate the progression of arthritis. Methods: In this in vivo study, 16-week-old male Sprague–Dawley rats were divided into three groups: Group 1 (sham control group), Group 2 (Rb1-treated group), and Group 3 (OA group). In Groups 2 and 3, OA was induced in the right knee joint with ACLT + MMx in rats. Then Group 2 received continuous infusion of ginsenoside Rb1 via osmotic mini-pumps implanted subcutaneously. At 4 weeks after treatment, the rats were sacrificed. Interleukin-1β (IL-1β) level was evaluated by enzyme-linked immunosorbent assay (ELISA); cartilage damage was assessed via histology (Safranin-O/fast green stain) and immunohistochemistry [matrix metalloproteinase-13 (MMP13) and type X collagen (Col X)]. For cell study, C5.18 (rat chondrocyte cell line) was used in this research. The effect of Rb1 on IL-1β-induced MMP13 or Col X expression level in C5.18 cells was investigated. Results: In this in vivo study, characteristics of OA were present in the OA group, in contrast to less severe damage generally observed in the Rb1 treatment group: first, IL-1β level was significantly decreased, and second, cartilage degeneration was attenuated, as indicated by lower histologic damage scores and lower percentages of MMP13 or Col X-positive chondrocytes. In the cell study, the results showed that Rb1 treatment would relieve the MMP13 or Col X expression in C5.18 cells induced by IL-1β. Conclusion: In the present study, we demonstrated that Rb1 can attenuate the progression or severity of arthritis by reducing inflammation

Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles

Truffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles. Three polysaccharide fractions were obtained from Tuber panzhihuanense and referred to as TPZ-NP, TPZ-I, and TPZ-II. Additionally, two polysaccharide fractions were purified from T. pseudoexcavatum (TPD-NP and TPD-I). The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight. Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing. The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression. The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure. Further study on the structure-activity relationship is needed to be done