Research on Keyhole Diameter’s Vision Measurement Based on Parallel Technology

A keyhole diameter of the cartridge’s measurement based on machine vision is a part of the cartridge’s geometry measurement system, according to the system requirements, to complete the measurement within 5 seconds. A Image Collection System was constructed using computer, CCD camera, LED source, meanwhile a measurement system was compiled by C# on VS2010 platform based on machine vision. Use the Otsu algorithm to extracts the keyhole’s edge and near the pixels in order to reduce the computational Canny operator, and use parallel computing in the Canny operator to improve computing speed purposes. Use QueryPerformanceCounter timer for each module timing Canny operator, Canny operator improved computation time is reduced from the original 6s to nearly a hundred ms improved. Meet the time requirements of cartridge geometry measurement system, and other machine vision in which the project can be widely used

Compression Behaviour of Natural and Reconstituted Clays

International audienceThe intercept of the log(1+e) - logσv' straight line is introduced to describe the effect of the starting point on the compressibility of natural and reconstituted clays. It is found that when the effective stress exceeds the remoulded yield stress, the compression behaviour of reconstituted clays is controlled solely by the water content at the remoulded yield stress and the liquid limit. Comparison of the compression behaviour of natural and reconstituted clays indicates that their difference in compressibility is caused by soil structure and the difference in water content at the compression starting point. The compression behaviour of natural clays can be classified into three regimes: 1) the pre-yield regime characterised by small compressibility with soil structure restraining the deformation up to the consolidation yield stress; 2) the transitional regime characterised by a gradual loss of soil structure when the effective stress is between the consolidation yield stress and the transitional stress; and 3) the post-transitional regime characterised by the same change law in compression behaviour as reconstituted clays when the effective stress is higher than the transitional stress. For the investigated clays, the transitional stress is 1.0-3.5 times the consolidation yield stress. The compression index varies solely with the void ratio at an effective stress of 1.0 kPa for both natural clays in post-transitional regime and reconstituted clays when the effective stress exceeds the remoulded yield stress, and when compressed in such cases the compression curves of both natural clays and reconstituted clays can be normalised well to a unique line using the void index

Regulation of Proapoptotic Mammalian ste20–Like Kinase MST2 by the IGF1-Akt Pathway

Hippo, a Drosophila serine/threonine kinase, promotes apoptosis and restricts cell growth and proliferation. Its mammalian homolog MST2 has been shown to play similar role and be regulated by Raf-1 via a kinase-independent mechanism and by RASSF family proteins through forming complex with MST2. However, regulation of MST2 by cell survival signal remains largely unknown.Using immunoblotting, in vitro kinase and in vivo labeling assays, we show that IGF1 inhibits MST2 cleavage and activation induced by DNA damage through the phosphatidylinosotol 3-kinase (PI3K)/Akt pathway. Akt phosphorylates a highly conserved threonine-117 residue of MST2 in vitro and in vivo, which leads to inhibition of MST2 cleavage, nuclear translocation, autophosphorylation-Thr180 and kinase activity. As a result, MST2 proapoptotic and growth arrest function was significantly reduced. Further, inverse correlation between pMST2-T117/pAkt and pMST2-T180 was observed in human breast tumors.Our findings demonstrate for the first time that extracellular cell survival signal IGF1 regulates MST2 and that Akt is a key upstream regulator of MST2

Poly[[(1,10-phenanthroline){μ3-2,2′,2′′-[1,3,5-triazine-2,4,6-triyltris(sulfane­diyl)]triacetato}­cadmium] 0.42-hydrate]

The asymmetric unit of the title complex, {[Cd(C9H7N3O6S3)(C12H8N2)]·0.42H2O}n, contains a CdII atom, one doubly deprotonated 2,2′,2′′-[1,3,5-triazine-2,4,6-triyltris(sulfanediyl)]triacetic acid ligand (HTTTA2−), a 1,10-phenanthroline (phen) ligand and a fractionally occupied water mol­ecule [site occupancy = 0.421 (15)]. The CdII atom is six-coordinated within a distorted octa­hedral coordination geometry. Six coordination arises from four O atoms derived from three different HTTTA2− ligands, and two N atoms of the chelating phen mol­ecule. The incompletely deprotonated triazine ligand adopts a μ3-η1:η1:η2 coordination mode, resulting in the formation of chains along the c axis based on Cd2O2 dimeric units. Adjacent chains are stacked through π–π stacking [3.533 (2) Å between phen and triazine rings] and C—H⋯O inter­actions, forming supra­molecular sheets in the ab plane. Intra-and intermolecular O—H⋯O hydrogen bonds are also observed

Oral microbiome-driven virulence factors: A novel approach to pancreatic cancer diagnosis

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy, often associated with a poor prognosis for patients. One of the major challenges in managing PDAC is the difficulty in early diagnosis, owing to the limited and invasive nature of current diagnostic methods. Recent studies have identified the oral microbiome as a potential source of non-invasive biomarkers for diseases, including PDAC. In this study, we focused on leveraging the differential expression of virulence factors (VFs) encoded by the oral microbiome to create a diagnostic tool for PDAC. We observed a higher alpha diversity in VF categories among PDAC patients compared to healthy controls. We then identified a panel of VF categories that were significantly upregulated in PDAC patients, these being associated with bacterial adherence, exoenzyme production, and nutritional/metabolic processes. Moreover, Streptococcus-derived VFs were notably enriched in PDAC patients. We developed a diagnostic model using random forest analysis based on the levels of these VFs. The model's diagnostic accuracy was evaluated using receiver operating characteristic (ROC) curve analysis, with an area under the curve (AUC) of 0.88, indicating high accuracy in differentiating PDAC patients from healthy controls. Our findings suggest that VFs encoded by the oral microbiome hold potential as diagnostic tools for PDAC, offering a non-invasive approach that could significantly enhance early detection and prognosis, ultimately leading to improved patient outcomes

Cyclodepsipeptide Toxin Promotes the Degradation of Hsp90 Client Proteins through Chaperone-Mediated Autophagy

Promoting the degradation of Hsp90 client proteins by inhibiting Hsp90, an important protein chaperone, has been shown to be a promising new anticancer strategy. In this study, we show that an oxazoline analogue of apratoxin A (oz-apraA), a cyclodepsipeptide isolated from a marine cyanobacterium, promotes the degradation of Hsp90 clients through chaperone-mediated autophagy (CMA). We identify a KFERQ-like motif as a conserved pentapeptide sequence in the kinase domain of epidermal growth factor receptor (EGFR) necessary for recognition as a CMA substrate. Mutation of this motif prevents EGFR degradation by CMA and promotes the degradation of EGFR through the proteasomal pathway in oz-apraA–treated cells. Oz-apraA binds to Hsc70/Hsp70. We propose that apratoxin A inhibits Hsp90 function by stabilizing the interaction of Hsp90 client proteins with Hsc70/Hsp70 and thus prevents their interactions with Hsp90. Our study provides the first examples for the ability of CMA to mediate degradation of membrane receptors and cross talks of CMA and proteasomal degradation mechanisms