Results of Controlled tissue regeneration in surgery for diabetic foot syndrome

Relevance. The problem of tissue regeneration in diabetic foot syndrome has currently become particularly relevant due to the multipolarity of the mechanisms of trophic disorders, their tendency to recur, the expansion of the affected areas when using traditional treatment methods, resistance to drug treatment methods, deterioration of patients' quality of life, as well as due to the significant expenditure of time and effort required for treatment. All these factors lead to significant economic costs in the management of patients in this cohort.The aim of the study: to develop new combined methods of surgical treatment of tissue defects in patients with complicated forms of diabetic foot syndrome.Materials and methods. The paper presents an original approach to solving one of the urgent problems of modern surgery of complications of diabetes mellitus, to the treatment of pathological processes of soft tissues of the lower extremities.Conclusions. At the stages of the entire study period, rapid wound healing was registered regardless of the initial clinical data and patient's medical history. There was also a decrease in the inflammatory response and active restoration of damaged tissues

An oligonucleotide hybridization approach to DNA sequencing

AbstractWe have proposed a DNA sequencing method based on hybridization of a DNA fragment to be sequenced with the complete set of fixed-length oligonucleotides (e.g., 48 = 65 536 possible 8-mers) immobilized individually as dots of a 2-D matrix [(1989) Dokl. Akad. Nauk SSSR 303, 1508-1511]. It was shown that the list of hybridizing octanucleotides is sufficient for the computer-assisted reconstruction of the structures for 80% of random-sequence fragments up to 200 bases long, based on the analysis of the octanucleotide overlapping. Here a refinement of the method and some experimental data are presented. We have performed hybridizations with oligonucleotides immobilized on a glass plate, and obtained their dissociation curves down to heptanucleotides. Other approaches, e.g. an additional hybridization of short oligonucleotides which continuously extend duplexes formed between the fragment and immobilized oligonucleotides, should considerably increase either the probability of unambiguous reconstruction, or the length of reconstructed sequences, or decrease the size of immobilized oligonucleotides.DNA sequencing; Oligonucleotide hybridization, immobilize

Temporomandibular joint's reconstruction after segmental mandibulectomy in patients with primary and secondary tumors of the mandible

Background: Auto and allografts are used for segmental resection of the mandible with its exarticulation and simultaneous reconstruction. Endoprosthetic replacement of temporomandibular joint (TMJ) may bring good functional results. However, some complications, such as fracture of the fixing part of the endoprosthesis, migration of its head into the middle cranial fossa and prosthesis eruption, can occur in the long-term. The use of revascularized bone autografts allow for replacement of the mandibular defect and to restore TMJ function. Aim: To evaluate functional, aesthetic and oncological results after segmental resection of the mandible with its exarticulation and simultaneous reconstruction with allografts and revascularized bone autografts. Materials and methods: Thirty patients were enrolled into the study, 22 of them being with primary mandibular tumors and 8 with oral cancers originating from mucosa, with advanced involvement of the mandible. Segmental mandibulectomy with simultaneous reconstruction was performed in all patients, with 9 of them having the allograft and endoprosthesis of the articular head and 21 patients having revascularized bone or combined grafts. If only a defect of the mandibular ramus and articular head was to be replaced, we used an iliac free flap (n = 5), whereas for replacement of a defect of the mandibular ramus, body and articular head a fibular free flap was implanted (n = 16). Results: The use of allografts was associated with 4 (44.4%) complication events, such as plate fracture (n = 2) at 2 and 6 years and eruption of the plate. When revascularized grafts were used, complete necrosis was seen in 1 (4.7%) case. The iliac graft was formed with the size of the ramus defect (most often, up to the mandibular angle), and the articular head was formed from the distal part. At least one osteotomy was performed in the fibular graft at the angle, and the articular head was formed in the distal part. Twenty (66.7%) patients are currently disease-free. Six (33.3%) patients died of relapse at 1 to 5 years, and 4 (13.3%) patients died with lung metastases of osteogenic sarcoma of the mandible. Conclusion: Allotransplantation after segmental resection of the mandible gives good functional results, although with a high rate of late complications (44.4%). In patients with limited defects of the mandibular ramus and head, revascularized iliac grafts can be used. In those with large defects, the method of choice is a fibular graft. It is possible to make the articular head of the distal end of the graft with its subsequent adaptation to the functional load