On the intrinsic bottom content of the nucleon and its impact on heavy new physics at the LHC

Heavy quark parton distribution functions (PDFs) play an important role in several Standard Model and New Physics processes. Most analyses rely on the assumption that the charm and bottom PDFs are generated perturbatively by gluon splitting and do not involve any non-perturbative degrees of freedom. It is clearly necessary to test this hypothesis with suitable QCD processes. Conversely, a non-perturbative, intrinsic heavy quark parton distribution has been predicted in the literature. We demonstrate that to a very good approximation the scale-evolution of the intrinsic heavy quark content of the nucleon is governed by non-singlet evolution equations. This allows us to analyze the intrinsic heavy quark distributions without having to resort to a full-fledged global analysis of parton distribution functions. We exploit this freedom to model intrinsic bottom distributions which are so far missing in the literature in order to estimate the impact of this non-perturbative contribution to the bottom-quark PDF, and on parton--parton luminosities at the LHC. This technique can be applied to the case of intrinsic charm, albeit within the limitations outlined in the following.Comment: 23 pages, 11 figure

Isolation and functional characterization of CE1 binding proteins

<p>Abstract</p> <p>Background</p> <p>Abscisic acid (ABA) is a plant hormone that controls seed germination, protective responses to various abiotic stresses and seed maturation. The ABA-dependent processes entail changes in gene expression. Numerous genes are regulated by ABA, and promoter analyses of the genes revealed that <it>cis</it>-elements sharing the ACGTGGC consensus sequence are ubiquitous among ABA-regulated gene promoters. The importance of the core sequence, which is generally known as ABA response element (ABRE), has been demonstrated by various experiments, and its cognate transcription factors known as ABFs/AREBs have been identified. Although necessary, ABRE alone is not sufficient, and another <it>cis</it>-element known as "coupling element (CE)" is required for full range ABA-regulation of gene expression. Several CEs are known. However, despite their importance, the cognate transcription factors mediating ABA response via CEs have not been reported to date. Here, we report the isolation of transcription factors that bind one of the coupling elements, CE1.</p> <p>Results</p> <p>To isolate CE1 binding proteins, we carried out yeast one-hybrid screens. Reporter genes containing a trimer of the CE1 element were prepared and introduced into a yeast strain. The yeast was transformed with library DNA that represents RNA isolated from ABA-treated Arabidopsis seedlings. From the screen of 3.6 million yeast transformants, we isolated 78 positive clones. Analysis of the clones revealed that a group of AP2/ERF domain proteins binds the CE1 element. We investigated their expression patterns and analyzed their overexpression lines to investigate the <it>in vivo </it>functions of the CE element binding factors (CEBFs). Here, we show that one of the CEBFs, AtERF13, confers ABA hypersensitivity in Arabidopsis, whereas two other CEBFs enhance sugar sensitivity.</p> <p>Conclusions</p> <p>Our results indicate that a group of AP2/ERF superfamily proteins interacts with CE1. Several CEBFs are known to mediate defense or abiotic stress response, but the physiological functions of other CEBFs remain to be determined. Our <it>in vivo </it>functional analysis of several CEBFs suggests that they are likely to be involved in ABA and/or sugar response. Together with previous results reported by others, our current data raise an interesting possibility that the coupling element CE1 may function not only as an ABRE but also as an element mediating biotic and abiotic stress responses.</p

Neutrino interactions and nuclear effects in oscillation experiments and the nonperturbative dispersive sector in strong (quasi-)Abelian fields

We present in the first part cross sections for single pion production, quasi-elastic and deep inelastic scattering by muon and tau-neutrinos relevant for oscillation experiments, including nuclear effects. It will be useful for the study of neutrino oscillations in future oscillation experiments. In the second part we introduce a method to separate perturbative and non-perturbative parts in the dispersive sector of the Euler-Heisenberg Lagrangian density in strong (quasi-)Abelian fields. Further, using (modified) Borel-Pade approximation applied to the Euler-Heisenberg Lagrangian, we test numerically the approximate analytic continuation of the perturbative results into the nonperturbative sector

Differential screening identifies transcripts with depot-dependent expression in white adipose tissues

<p>Abstract</p> <p>Background</p> <p>The co-morbidities of obesity are tied to location of excess fat in the intra-abdominal as compared to subcutaneous white adipose tissue (WAT) depot. Genes distinctly expressed in WAT depots may impart depot-dependent physiological functions. To identify such genes, we prepared subtractive cDNA libraries from murine subcutaneous (SC) or intra-abdominal epididymal (EP) white adipocytes.</p> <p>Results</p> <p>Differential screening and qPCR validation identified 7 transcripts with 2.5-fold or greater enrichment in EP <it>vs</it>. SC adipocytes. Boc, a component of the hedgehog signaling pathway demonstrated highest enrichment (~12-fold) in EP adipocytes. We also identified a dramatic enrichment in SC adipocytes <it>vs</it>. EP adipocytes and in SC WAT <it>vs</it>. EP WAT for transcript(s) for the major urinary proteins (Mups), small secreted proteins with pheromone functions that are members of the lipocalin family. Expression of Boc and Mup transcript was further assessed in murine tissues, adipogenesis models, and obesity. qPCR analysis reveals that EP WAT is a major site of expression of Boc transcript. Furthermore, Boc transcript expression decreased in obese EP WAT with a concomitant upregulation of Boc transcript in the obese SC WAT depot. Assessment of the Boc binding partner Cdon in adipose tissue and cell fractions thereof, revealed transcript expression similar to Boc; suggestive of a role for the Boc-Cdon axis in WAT depot function. Mup transcripts were predominantly expressed in liver and in the SC and RP WAT depots and increased several thousand-fold during differentiation of primary murine preadipocytes to adipocytes. Mup transcripts were also markedly reduced in SC WAT and liver of <it>ob/ob </it>genetically obese mice compared to wild type.</p> <p>Conclusion</p> <p>Further assessment of WAT depot-enriched transcripts may uncover distinctions in WAT depot gene expression that illuminate the physiological impact of regional adiposity.</p