Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells

Abstract Background Interleukin-24(IL-24), also referred to as melanoma differentiation-associated gene-7(mda-7), is a unique member of the IL-10 gene family, which displays nearly ubiquitous cancer-specific toxicity. The most notable feature of IL-24 is selectively induced growth suppression and apoptosis in various cancer cells, with no harmful effects toward normal cells. Autophagy is a self-protective mechanism in many kinds of tumor cells that respond to anticancer treatment. It is reported that autophagy inhibition could enhance the effects of many kinds of anticancer treatments, including gene therapy. However, whether IL-24 is effective to treat oral squamous cell carcinomas (OSCC) and if autophagy inhibition could improve the anticancer effect of IL-24 towards OSCC is has not been detected. Methods MTT assays were carried out to determine the cell proliferation; Transfection was used to gene transfer; Western Blot was performed to detect the protein level of LC3II, P62, Beclin 1, Cleaved caspase-3, β-Tubulin and β-actin; Apoptosis rates and cell cycle alteration were analyzed using flow cytometry; Autophagy induction was confirmed by MDC staining, GFP-LC3 staining and transmission electron microscopy. Amount of IL-24 in the culture medium was quantified by ELISA. Apoptosis in vivo was analyzed by TUNEL assay. HE staining was used to observe the morphology of the samples. Results In the present study, we proved that IL-24 have a novel anticancer effect towards KB cells and that autophagy inhibition could improve the anticancer effect of IL-24. IL-24 treated cells showed autophagy characteristics and autophagy inhibition by 3-methyladenine (3-MA) significantly enhanced IL-24-induced apoptosis. Similar results were obtained in the KB cells xenograft tumor model. Conclusions These results suggest that the combination of autophagy inhibitors and IL-24 based on the AdLTR2EF1α-mediated gene transfer could be a promising way to cure OSCC.http://deepblue.lib.umich.edu/bitstream/2027.42/113230/1/13046_2015_Article_211.pd

Dynamic Complexity of a Phytoplankton-Fish Model with the Impulsive Feedback Control by means of Poincaré Map

The phytoplankton-fish model for catching fish with impulsive feedback control is established in this paper. Firstly, the Poincaré map for the phytoplankton-fish model is defined, and the properties of monotonicity, continuity, differentiability, and fixed point of Poincaré map are analyzed. In particular, the continuous and discontinuous properties of Poincaré map under different conditions are discussed. Secondly, we conduct the analysis of the necessary and sufficient conditions for the existence, uniqueness, and global stability of the order-1 periodic solution of the phytoplankton-fish model and obtain the sufficient conditions for the existence of the order-kk≥2 periodic solution of the system. Numerical simulation shows the correctness of our results which show that phytoplankton and fish with the impulsive feedback control can live stably under certain conditions, and the results have certain reference value for the dynamic change of phytoplankton in aquatic ecosystems

Dynamic Analysis of Beddington–DeAngelis Predator-Prey System with Nonlinear Impulse Feedback Control

In this paper, a predator-prey system with pesticide dose-responded nonlinear pulse of Beddington–DeAngelis functional response is established. First, we construct the Poincaré map of the impulsive semidynamic system and discuss its main properties including the monotonicity, differentiability, fixed point, and asymptote. Second, we address the existence and globally asymptotic stability of the order-1 periodic solution and the sufficient conditions for the existence of the order-k(k ≥ 2) periodic solution. Thirdly, we give the threshold conditions for the existence and stability of boundary periodic solutions and present the parameter analysis. The results show that the pesticide dosage increases with the extension of the control period and decreases with the increase of the threshold. Besides, the state pulse feedback control can manage the pest population at a certain level and avoid excessive application of pesticides

Experimental Study on the Influence of Microwave Energy Pulse Width and Duty Cycle on Evaporation and Ignition Characteristics of ADN-Based Liquid Propellant Droplets

This study investigates the evaporation and ignition characteristics of a single droplet of ammonium dinitramide (ADN)-based liquid propellant utilizing a waveguide resonant cavity device, in conjunction with a high-speed photographic imaging system and testing system. Experimental methods are employed to analyze the impact of microwave pulse width and duty cycle on the puffing and meicro-explosion phenomena of the droplet, as well as the delay time and duration of ignition. The experimental findings reveal that increasing the duty cycle enhances the ignition success rate and diminishes flame development time. Specifically, elevating the microwave duty cycle from 60% to 80% reduces the ignition delay time of the droplet from 132.8 ms to 88.1 ms, and the ignition duration from 23.1 ms to 19.9 ms. Furthermore, an increase in microwave energy pulse width expedites the combustion process of the flame and influences plasma generation. Increasing the pulse width of microwave energy from 20 µs to 40 µs prolongs the ignition delay time from 140.3 ms to 200.5 ms and extends the ignition duration from 56.7 ms to 77.8 ms. Additionally, it is observed that a higher duty cycle leads to a more pronounced puffing phenomenon that initiates earlier. In contrast, a higher pulse width results in a more pronounced puffing phenomenon that commences later. This study provides a thorough investigation into the microwave ignition mechanism of ADN-based liquid propellants, offering theoretical insights into the ignition and combustion stability of such propellants in microwave-assisted ignition systems

HMM-Based Method for Aircraft Environmental Control System Turbofan Rolling Bearing Fault Diagnosis

In response to the high-noise, nonlinear, and nonstationary characteristics of vibration signals from aircraft environmental control system (ECS) turbofan rolling bearings, this paper proposes a diagnostic method for the degree of ECS turbofan bearing faults based on the Hidden Markov Model (HMM). Experimental results demonstrate that HMM can accurately diagnose and predict faults in ECS turbofan rolling bearings. The HMM method enhances diagnostic accuracy, and its effectiveness and feasibility in fault diagnosis based on different rolling bearing fault instances are elaborated. By employing the HMM model to establish precise models from decomposed dynamic data, it successfully identifies faults such as the fracture of the bearing cage under biased load conditions, although its performance in recognizing overheating faults is suboptimal

Atmospheric-Window-Matching Hierarchical Broadband Infrared Absorber Realized by Lithography-Free Fabrication

An ultra-broadband selective absorber has been realized with a hierarchical structure through integrating vacuum impedance-matched structure, quarter wavelength structure, and gradient refractive index structure. Through optimizing the design parameters of the proposed hierarchical structure, an ultra-broadband infrared absorber covering the three major atmospheric windows (0.7–2.5, 3–5, and 8–14 μm) has been numerically and experimentally demonstrated. An overall absorption up to 80% covering all the three major atmospheric infrared windows and a ratio of the total absorptions within and beyond the windows as high as 5.88 has been achieved with the developed absorber. The high absorption and spectral selectivity of the absorber make it promising for sensitive broadband infrared spectroscopy detection. The proposed hierarchical structure also provides great design freedom with many tunable factors, making it convenient to extend the design for other applications. In addition, we developed a cost-effective lithography-free method for the fabrication of this structure. The design flexibility and fabrication convenience of this hierarchical structure render it suitable for the development of tailored selective broadband absorbers for targeted applications