Premeditations of performance in recent live television

The article discusses the ways that performances in television are premeditated. Its focus is on practices of scripting performances in current interactive and reality TV. With a basis in empirical material on television production, the article describes concretely some cases of scripting in international television formats. Its focus is particularly on forms of scripting that are seldom recognized as such, thus contributing to a feel of the real, the authentic and the immediate. Three forms of scripting are discussed: ‘cueing’, scripting of social settings and scripting of temporal sequencings. The article shows how current television formats that strive for the unscripted feel in fact could be said to involve radical extensions of scripting beyond conventional practices. In more general theoretical terms, the article suggests looking to the scripting of mediated performances as an alternative approach to the tradition of media production studies

Absolute FKBP binding affinities obtained via non-equilibrium unbinding simulations

We compute absolute binding affinities for two ligands bound to the FKBP protein using non-equilibrium unbinding simulations. The methodology is straight-forward, requiring little or no modification to many modern molecular simulation packages. The approach makes use of a physical pathway, eliminating the need for complicated alchemical decoupling schemes. Results of this study are promising. For the ligands studied here the binding affinities are typically estimated within less than 4.0 kJ/mol of the target values; and the target values are within less than 1.0 kJ/mol of experiment. These results suggest that non-equilibrium simulation could provide a simple and robust means to estimate protein-ligand binding affinities.Comment: 9 pages, 3 figures (no necessary color). Changes made to methodology and results between revision