115 research outputs found
A Bayesian method for inferring quantitative information from FRET data
<p>Abstract</p> <p>Background</p> <p>Understanding biological networks requires identifying their elementary protein interactions and establishing the timing and strength of those interactions. Fluorescence microscopy and Förster resonance energy transfer (FRET) have the potential to reveal such information because they allow molecular interactions to be monitored in living cells, but it is unclear how best to analyze FRET data. Existing techniques differ in assumptions, manipulations of data and the quantities they derive. To address this variation, we have developed a versatile Bayesian analysis based on clear assumptions and systematic statistics.</p> <p>Results</p> <p>Our algorithm infers values of the FRET efficiency and dissociation constant, <it>K<sub>d</sub></it>, between a pair of fluorescently tagged proteins. It gives a posterior probability distribution for these parameters, conveying more extensive information than single-value estimates can. The width and shape of the distribution reflects the reliability of the estimate and we used simulated data to determine how measurement noise, data quantity and fluorophore concentrations affect the inference. We are able to show why varying concentrations of donors and acceptors is necessary for estimating <it>K<sub>d</sub></it>. We further demonstrate that the inference improves if additional knowledge is available, for example of the FRET efficiency, which could be obtained from separate fluorescence lifetime measurements.</p> <p>Conclusions</p> <p>We present a general, systematic approach for extracting quantitative information on molecular interactions from FRET data. Our method yields both an estimate of the dissociation constant and the uncertainty associated with that estimate. The information produced by our algorithm can help design optimal experiments and is fundamental for developing mathematical models of biochemical networks.</p
The chlL ( frxC ) gene: Phylogenetic distribution in vascular plants and DNA sequence from Polystichum acrostichoides ( Pteridophyta ) and Synechococcus sp. 7002 ( Cyanobacteria )
We examined chlL ( frxC ) gene evolution using several approaches. Sequences from the chloroplast genome of the fern Polystichum acrostichoides and from the cyanobacterium Synechococcus sp. 7002 were determined and found to be highly conserved. A complete physical map of the fern chloroplast genome and partial maps of other vascular plant taxa show that chlL is located primarily in the small single copy region as in Marchantia polymorpha. A survey of a wide variety of non-angiospermous vascular plant DNAs shows that chlL is widely distributed but has been lost in the pteridophyte Psilotum and (presumably independently) within the Gnetalean gymnosperms.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41636/1/606_2004_Article_BF00994092.pd
Light-harvesting II (B800-B850 complex) structural genes from Rhodopseudomonas capsulata
Reverse transcriptase pauses at N2-methylguanine during in vitro transcription of Escherichia coli 16S ribosomal RNA.
Directed mutations affecting spectroscopic and electron transfer properties of the primary donor in the photosynthetic reaction center
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