The Light and Period Variations of the Eclipsing Binary BX Draconis

New CCD photometric observations of BX Dra were obtained for 26 nights from 2009 April to 2010 June. The long-term photometric behaviors of the system are presented from detailed studies of the period and light variations, based on the historical data and our new observations. All available light curves display total eclipses at secondary minima and inverse O'Connell effects with Max I fainter than Max II, which are satisfactorily modeled by adding the slightly time-varying hot spot on the primary star. A total of 87 times of minimum light spanning over about 74 yrs, including our 22 timing measurements, were used for ephemeris computations. Detailed analysis of the O-C diagram showed that the orbital period has changed in combinations with an upward parabola and a sinusoidal variation. The continuous period increase with a rate of +5.65 \times 10^-7 d yr^-1 is consistent with that calculated from the Wilson-Devinney synthesis code. It can be interpreted as a mass transfer from the secondary to the primary star at a rate of 2.74 \times 10^-7 M\odot yr^-1, which is one of the largest rates for contact systems. The most likely explanation of the sinusoidal variation with a period of 30.2 yrs and a semi-amplitude of 0.0062 d is a light-traveltime effect due to the existence of a circumbinary object. We suggest that BX Dra is probably a triple system, consisting of a primary star with a spectral type of F0, its secondary component of spectral type F1-2, and an unseen circumbinary object with a minimum mass of M3 = 0.23 M\odot.Comment: 24 pages, including 5 figures and 9 tables, accepted for publication in PAS

Hydrogen Cyanide Produced by Pseudomonas chlororaphis O6 Exhibits Nematicidal Activity against Meloidogyne hapla

Root-knot nematodes (Meloidogyne spp.) are parasites that attack many field crops and orchard trees, and affect both the quantity and quality of the products. A root-colonizing bacterium, Pseudomonas chlororaphis O6, possesses beneficial traits including strong nematicidal activity. To determine the molecular mechanisms involved in the nematicidal activity of P. chlororaphis O6, we constructed two mutants; one lacking hydrogen cyanide production, and a second lacking an insecticidal toxin, FitD. Root drenching with wild-type P. chlororaphis O6 cells caused juvenile mortality in vitro and in planta. Efficacy was not altered in the fitD mutant compared to the wild-type but was reduced in both bioassays for the mutant lacking hydrogen cyanide production. The reduced number of galls on tomato plants caused by the wild-type strain was comparable to that of a standard chemical nematicide. These findings suggest that hydrogen cyanide-producing root colonizers, such as P. chlororaphis O6, could be formulated as “green” nematicides that are compatible with many crops and offer agricultural sustainability

A New SX Phe Star in the Globular Cluster M15

A new SX Phe star (labelled SXP1) found from $BV$ CCD photometry is the first to be discovered in the globular cluster M15. It is a blue straggler and is located 102\arcsec.8 north and 285\arcsec.6 west of the center of M15 \citep{har96}. Mean magnitudes of SXP1 are = 18$\fm$671 and = 18\fm445. The amplitude of variability of SXP1 is measured to be $\Delta V \approx 0.15$. From multiple-frequency analysis based on the Fourier decomposition method, we detect two very closely separated pulsating frequencies: the primary frequency at $f_1=24.630$ c/d for both $B$- and $V$-bands, and the secondary frequency at $f_2=24.338$ c/d for the $B$-band and 24.343 c/d for the $V$-band. This star is the second among known SX Phe stars found to pulsate with very closely separated frequencies ($f_2/f_1\ge0.95$). These frequencies may be explained by excitation of nonradial modes; however, we have an incomplete understanding of this phenomenon in the case of SX Phe stars with relatively high amplitudes. The metallicity-period and the variability amplitude-period relations for SXP1 in M15 are found to be consistent with those for SX Phe stars in other globular clusters.Comment: 15 pages with 6 figures, accepted by the Astronomical Journal (scheduled May 2001

Precision genome engineering with programmable DNA-nicking enzymes

Zinc finger nucleases (ZFNs) are powerful tools of genome engineering but are limited by their inevitable reliance on error-prone nonhomologous end-joining (NHEJ) repair of DNA double-strand breaks (DSBs), which gives rise to randomly generated, unwanted small insertions or deletions (indels) at both on-target and off-target sites. Here, we present programmable DNA-nicking enzymes (nickases) that produce single-strand breaks (SSBs) or nicks, instead of DSBs, which are repaired by error-free homologous recombination (HR) rather than mutagenic NHEJ. Unlike their corresponding nucleases, zinc finger nickases allow site-specific genome modifications only at the on-target site, without the induction of unwanted indels. We propose that programmable nickases will be of broad utility in research, medicine, and biotechnology, enabling precision genome engineering in any cell or organism.

Effects of strobe light stimulation on postnatal developing rat retina

The nature and intensity of visual stimuli have changed in recent years because of television and other dynamic light sources. Although light stimuli accompanied by contrast and strength changes are thought to have an influence on visual system development, little information is available on the effects of dynamic light stimuli such as a strobe light on visual system development. Thus, this study was designed to evaluate changes caused by dynamic light stimuli during retinal development. This study used 80 Sprague-Dawley rats. From eye opening (postnatal day 14), half of the rats were maintained on a daily 12-h light/dark cycle (control group) and the remaining animals were raised under a 12-h strobe light (2 Hz)/dark cycle (strobe light-reared group). Morphological analyses and electroretinogram (ERG) were performed at postnatal weeks 3, 4, 6, 8, and 10. Among retinal neurons, tyrosine hydroxylase-immunoreactive (TH-IR, dopaminergic amacrine cells) cells showed marked plastic changes, such as variations in numbers and soma sizes. In whole-mount preparations at 6, 8, and 10 weeks, type I TH-IR cells showed a decreased number and larger somata, while type II TH-IR cells showed an increased number in strobe-reared animals. Functional assessment by scotopic ERG showed that a-wave and b-wave amplitudes increased at 6 and 8 weeks in strobe-reared animals. These results show that exposure to a strobe light during development causes changes in TH-IR cell number and morphology, leading to a disturbance in normal visual functions