Self-regulated mechanism of Plk1 localization to kinetochores: lessons from the Plk1-PBIP1 interaction

Mammalian polo-like kinase 1 (Plk1) has been studied extensively as a critical element in regulating various mitotic events during M-phase progression. Plk1 function is spatially regulated through the targeting activity of the conserved polo-box domain (PBD) present in the C-terminal non-catalytic region. Recent progress in our understanding of Plk1 localization to the centromeres shows that Plk1 self-regulates its initial recruitment by phosphorylating a centromeric component PBIP1 and generating its own PBD-binding site. Paradoxically, Plk1 also induces PBIP1 delocalization and degradation from the mitotic kinetochores late in the cell cycle, consequently permitting itself to bind to other kinetochore components. Thus, PBIP1-dependent self-recruitment of Plk1 to the interphase centromeres serves as a prelude to the efficient delivery of Plk1 itself to other kinetochore components whose interactions with Plk1 are vital for proper mitotic progression

Surface and bottom temperature and salinity climatology along the continental shelf off the Canadian and U.S. East Coasts

© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Continental Shelf Research 124 (2016): 165-181, doi:10.1016/j.csr.2016.06.005.A new hydrographic climatology has been created for the continental shelf region, extending from the Labrador shelf to the Mid-Atlantic Bight. The 0.2-degree climatology combines all available observations of surface and bottom temperature and salinity collected between 1950 and 2010 along with the location, depth and date of these measurements. While climatological studies of surface and bottom temperature and salinity have been presented previously for various regions along the Canadian and U.S. shelves, studies also suggest that all these regions are part of one coherent system. This study focuses on the coherent structure of the mean seasonal cycle of surface and bottom temperature and salinity and its variation along the shelf and upper slope. The seasonal cycle of surface temperature is mainly driven by the surface heat flux and exhibits strong dependency on latitude (r≈−0.9). The amplitude of the seasonal cycle of bottom temperature is rather dependent on the depth, while the spatial distribution of bottom temperature is correlated with latitude. The seasonal cycle of surface salinity is influenced by several components, such as sea-ice on the northern shelves and river discharge in the Gulf of St. Lawrence. The bottom salinity exhibits no clear seasonal cycle, but its spatial distribution is highly correlated with bathymetry, thus Slope Water and its intrusion on the shelf can be identified by its relatively high salinity compared to shallow, fresher shelf water. Two different regimes can be identified, especially on the shelf, separated by the Laurentian Channel: advection influences the phasing of the seasonal cycle of surface salinity and bottom temperature to the north, while in the southern region, river runoff and air-sea heat flux forcing are dominant, especially over the shallower bathymetry.Support from NSF OCE PO to Y-OK (OCE-1242989 and OCE-1435602) and SJL (OCE-1332666)

Diagnosis of Metastatic Breast Cancer to an Intraabdominal Lymph Node by Endoscopic Ultrasound

Breast cancer can present with metastatic disease initially or as a systemic relapse despite seemingly adequate initial treatment. We report a case of suspected metastatic breast cancer to an intraabdominal lymph node based on imaging, which was subsequently confirmed by tissue sampling at the time of endoscopic ultrasound (EUS). While previous studies have shown the utility of EUS in the diagnosis of metastatic breast cancer, this is the first case to our knowledge that describes the use of EUS in diagnosing recurrent breast cancer to an intraabdominal lymph node

Distinct mechanisms of decadal subsurface heat content variations in the eastern and western Indian Ocean modulated by tropical Pacific SST

Author Posting. © American Meteorological Society, 2018. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 31 (2018): 7751-7769, doi:10.1175/JCLI-D-18-0184.1.Decadal variability of the subsurface ocean heat content (OHC) in the Indian Ocean is investigated using a coupled climate model experiment, in which observed eastern tropical Pacific sea surface temperature (EPSST) anomalies are specified. This study intends to understand the contributions of external forcing relative to those of internal variability associated with EPSST, as well as the mechanisms by which the Pacific impacts Indian Ocean OHC. Internally generated variations associated with EPSST dominate decadal variations in the subsurface Indian Ocean. Consistent with ocean reanalyses, the coupled model reproduces a pronounced east–west dipole structure in the southern tropical Indian Ocean and discontinuities in westward-propagating signals in the central Indian Ocean around 100°E. This implies distinct mechanisms by which the Pacific impacts the eastern and western Indian Ocean on decadal time scales. Decadal variations of OHC in the eastern Indian Ocean are attributed to 1) western Pacific surface wind anomalies, which trigger oceanic Rossby waves propagating westward through the Indonesian Seas and influence Indonesian Throughflow transport, and 2) zonal wind anomalies over the central tropical Indian Ocean, which trigger eastward-propagating Kelvin waves. Decadal variations of OHC in the western Indian Ocean are linked to conditions in the Pacific via changes in the atmospheric Walker cell, which trigger anomalous wind stress curl and Ekman pumping in the central tropical Indian Ocean. Westward-propagating oceanic Rossby waves extend the influence of this anomalous Ekman pumping to the western Indian Ocean.This research was supported by the Independent Research and Development Program at WHOI to CCU, an NSF OCE PO grant (NSF OCE- 1242989) to Young-Oh Kwon, NOAA CP CVP grants (NA15OAR4310176 and NA17OAR4310255) to Hyodae Seo, and a research grant fromtheMinistry of Science and Technology of the People’s Republic of China to Tsinghua University (2017YFA0603902).2019-02-1

Long-term SST variability on the Northwest Atlantic continental shelf and slope

Author Posting. © American Geophysical Union, 2020. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 47(1), (2020): e2019GL085455, doi:10.1029/2019GL085455.The meridional coherence, connectivity, and regional inhomogeneity in long‐term sea surface temperature (SST) variability over the Northwest Atlantic continental shelf and slope from 1982–2018 are investigated using observational data sets. A meridionally concurrent large SST warming trend is identified as the dominant signal over the length of the continental shelf and slope between Cape Hatteras in North Carolina and Cape Chidley, Newfoundland and Labrador, Canada. The linear trends are 0.37 ± 0.06 and 0.39 ± 0.06 °C/decade for the shelf and slope regions, respectively. These meridionally averaged SST time series over the shelf and slope are consistent with each other and across multiple longer observational data sets with records dating back to 1900. The coherence between the long‐term meridionally averaged time series over the shelf and slope and basin‐wide averaged SST in the North Atlantic implies approximately two thirds of the warming trend during 1982–2018 may be attributed to natural climate variability and the rest to externally forced change including anthropogenic warming.We are grateful to the Editor Dr. Kathleen Donohue and two anonymous reviewers. This work was supported by NOAA's Climate Program Office's Modeling, Analysis, Predictions, and Projections (MAPP) program (NA19OAR4320074). We acknowledge our participation in MAPP's Marine Prediction Task Force. The data of NOAA OISST used in this study are available at NOAA Earth System Research Laboratory (https://www.esrl.noaa.gov/psd/data/gridded/data.noaa.oisst.v2.highres.html). The HadISST data set is available at Met Office, Hadley Centre (https://www.metoffice.gov.uk/hadobs/hadisst/). The COBE SST and NOAA ERSST data sets are available at NOAA Earth System Research Laboratory's Physical Sciences Division (https://www.esrl.noaa.gov/psd/data/gridded/data.cobe.html; https://www.esrl.noaa.gov/psd/data/gridded/data.noaa.ersst.v5.html). The near‐surface air temperature is available at Global Historical Climatology Network‐Monthly Database (https://www.ncdc.noaa.gov/data‐access/land‐based‐station‐data/land‐based‐datasets/global‐historical‐climatology‐network‐monthly‐version‐4). The data of SSH are available at Copernicus Marine Environment Monitoring Service (http://marine.copernicus.eu/services‐portfolio/access‐to‐products/?option=com_csw&view=details&product_id=SEALEVEL_GLO_PHY_ L4_REP_OBSERVATIONS_008_047).2020-07-0

Variability of extracorporeal cardiopulmonary resuscitation utilization for refractory adult out-of-hospital cardiac arrest: an international survey study.

Objective: A growing interest in extracorporeal cardiopulmonary resuscitation (ECPR) as a rescue strategy for refractory adult out-of-hospital cardiac arrest (OHCA) currently exists. This study aims to determine current standards of care and practice variation for ECPR patients in the USA and Korea. Methods: In December 2015, we surveyed centers from the Korean Hypothermia Network (KORHN) Investigators and the US National Post-Arrest Research Consortium (NPARC) on current targeted temperature management and ECPR practices. This project analyzes the subsection of questions addressing ECPR practices. We summarized survey. Results: Overall, 9 KORHN and 4 NPARC centers reported having ECPR programs and had complete survey data available. Two KORHN centers utilized extracorporeal membrane oxygenation only for postarrest circulatory support in patients with refractory shock and were excluded from further analysis. Centers with available ECPR generally saw a high volume of OHCA patients (10/11 centers care for \u3e75 OHCA a year). Location of, and providers trained for cannulation varied across centers. All centers in both countries (KORHN 7/7, NPARC 4/4) treated comatose ECPR patients with targeted temperature management. All NPARC centers and four of seven KORHN centers reported having a standardized hospital protocol for ECPR. Upper age cutoff for eligibility ranged from 60 to 75 years. No absolute contraindications were unanimous among centers. Conclusion: A wide variability in practice patterns exist between centers performing ECPR for refractory OHCA in the US and Korea. Standardized protocols and shared research databases might inform best practices, improve outcomes, and provide a foundation for prospective studies