Systemic mucoid degeneration of the arterial tunica intima in a young dog

A 27-mo-old, spayed female mixed-breed dog was presented with left forelimb pain, which progressed to full thickness necrosis of the soft tissues of multiple limbs. Clinical imaging and postmortem examination suggested multiple large arterial thromboemboli. Histologic examination of vascular lesions revealed markedly thickened tunica intima with polypoid intraluminal projections, which partially to entirely occluded the arterial lumen. The expanded tunica intima was comprised of intimal accumulation of Alcian blue-positive matrix with scattered spindle-to-satellite cells. These cells were positive for von Willebrand factor and vimentin but negative for alpha-smooth muscle actin, suggesting endothelial origin. Deposition of the intimal mucoid matrix was observed in the elastic and muscular arteries associated with regional ischemic changes. Mucoid emboli, likely from fragmentation of proliferative intimal tissue, were identified in smaller vessels supplied by affected arteries. Based on these findings, we diagnosed systemic mucoid degeneration of the arterial tunica intima. Such systemic arterial degeneration characterized by deposition of mucoid matrix in the tunica intima has not been reported previously in dogs, to our knowledge, and should be distinguished from thromboembolism and other degenerative vascular diseases

Gross Cystic Disease Fluid Protein 15 in Stratum Corneum Is a Potential Marker of Decreased Eccrine Sweating for Atopic Dermatitis

<div><p>It is well known that eccrine sweating is attenuated in patients with atopic dermatitis (AD). We have reported by using proteome analysis that gross cystic disease fluid protein 15 (GCDFP15), a substance secreted from eccrine sweat glands, is decreased in tape-stripped stratum corneum (SC) samples from AD patients. The aim of this study was to evaluate GCDFP15 production by eccrine glands with SC samples and to assess sweating in AD. SC samples were obtained from 51 healthy control (HC) and 51 AD individuals. Sweat samples were from 18 HC and 12 AD subjects. GCDFP15 was quantified by ELISA. By immunohistochemistry, the expression of GCDFP15 in eccrine glands was examined in normal and AD skin specimens. To identify GCDFP15-producing cells, double immunofluorescence staining for GCDFP15 and S100 protein was performed in frozen sections. To address the mechanism underlying the decreased eccrine sweating in AD patients, we examined the expression of cholinergic receptor M3 (CHRM3), a receptor for acetylcholine-induced sweating, in eccrine sweat glands. The amounts of GCDFP15 in the SC extracts were significantly lower in AD than HC (<i>P</i> < 0.0001). The sweat samples from AD patients also had lower levels of GCDFP15 concentration (<i>P</i> < 0.05). Immunohistochemistry showed positive GCDFP15 staining in the eccrine gland secretory cells and the ductal and acrosyringial lumen in normal skin, but AD lacked clear staining. Immunofluorescence staining revealed that GCDFP15 was co-expressed with S100 protein, suggesting that the clear cell of eccrine glands produces GCDFP15. Finally, we found that the expression of CHRM3 was depressed in AD, suggesting contribution to the low sweating. The SC of AD patients contains a low amount of GCDFP15 due to both low sweating and low GCDFP15 concentration in the sweat. GCDFP15 in SC is a potential marker for dysregulated sweating in AD.</p></div

Collection of stratum corneum samples and sweat samples from healthy controls and atopic dermatitis cases.

<p>HC: healthy control.</p><p>AD: atopic dermatitis.</p><p>Collection of stratum corneum samples and sweat samples from healthy controls and atopic dermatitis cases.</p

GCDFP15 levels in stratum corneum and sweat.

<p>Stratum corneum (SC) extracts (A) and sweat samples (B) were obtained from healthy control (HC) and atopic dermatitis (AD) individuals (i; male and female, ii; male, iii; female). GCDFP15 in SC extracts and sweat was quantified by ELISA. The results are expressed in box plot. The rectangle spans the first quartile to the third quartile. A segment inside the rectangle shows the median, and whiskers above and below the box show the locations of the minimum and maximum.</p

Distribution patterns of GCDFP15, CEA, and S100 protein in eccrine gland secretory coil.

<p>(A) Double staining of CEA and GCDFP15 in the eccrine gland secretory coil. The expression of CEA is observed in whole eccrine gland secretory coil cells. (B) Double staining of S100 protein and GCDFP15. The expression of S100 protein is observed in a part of eccrine gland secretory coil cells as granular pattern. Colocalization of S100 protein and GCDFP15 is shown in yellow in the merged image. Bars indicate 50 μm.</p