CXCL12‐induced macropinocytosis modulates two distinct pathways to activate mTORC1 in macrophages

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141686/1/jlb0683.pd

Coordination of the Rab5 Cycle on Macropinosomes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/88083/1/j.1600-0854.2011.01280.x.pd

High purity NaI(Tl) scintillator to search for dark matter

A high purity and large volume NaI(Tl) scintillator was developed to search for cosmic dark matter. The required densities of radioactive impurities (RIs) such as U-chain, Th-chain are less than a few ppt to establish high sensitivity to dark matter. The impurity of RIs were effectively reduced by selecting raw materials of crucible and by performing chemical reduction of lead ion in NaI raw powder. The impurity of $^{226}$Ra was reduced less than 100 $\mu$Bq/kg in NaI(Tl) crystal. It should be remarked that the impurity of $^{210}$Pb, which is difficult to reduce, is effectively reduced by chemical processing of NaI raw powder down to less than 30 $\mu$Bq/kg. The expected sensitivity to cosmic dark matter by using 250 kg of the high purity and large volume NaI(Tl) scintillator (PICO-LON; Pure Inorganic Crystal Observatory for LOw-background Neutr(al)ino) is 7$\times$10$^{-45}$ cm$^{2}$ for 50 GeV$/c^{2}$ WIMPs.Comment: 6 pages, 2 Figures, Proceedings of International Symposium on Radiation Detectors and Their Uses (ISRD2016). Talk given on 19th Jan. 2016 by K.Fushimi. To be published in Proceedings will be published as JPS conference proceedings (2016

PICO-LON Project for WIMPs search

Highly segmented inorganic crystal has been shown to have good performance for dark matter search. The energy resolution of ultra thin and large area NaI(Tl) scintillator has been developed. The estimated sensitivity for spin-dependent excitation of 127I was discussed. The recent status of low background measurement at Oto Cosmo Observatory is reportedComment: 3 pages, 1 figure, Proceedings of TAUP200

Fluorescence-guided bone resection by using Visually Enhanced Lesion Scope in diffuse chronic sclerosingosteomyelitis of the mandible: clinical and pathological evaluation

Diffuse chronic sclerosingosteomyelitis (DCSO) is a refractory disease, becausethe etiology and pathogenesis remain poorly understood and to determine the border betweenunhealthy boneandhealthybone is difficult. However, progressive inflammation, clinical symptoms and a high recurrence rate of DCSO were the reasons for surgical treatment. We report a case of a 66-year old woman with DCSO of the right side of mandible who was treated with hemimandibulectomy and simultaneous reconstruction by vascularized free fibula flap. After preoperative administration of minocycline for 1 month, the bone fluorescence was successfully monitored by using a Visually Enhanced Lesion Scope (VELscope®). Intraoperatively, we could determine the resection boundaries. We investigated the clinical and histopathological findings. The fluorescence findings were well correlated with histopathological findings. Using a VELscope®was handy and useful to determine the border between DCSO lesion andhealthybone. The free fibula flap under the minocycline-derived bone fluorescence by using a VELscope®offered a good quality of mandibular bone and the successful management of an advanced and refractory DCSO

Novel HER2 selective tyrosine kinase inhibitor, TAK-165, inhibits bladder, kidney and androgen-independent prostate cancer in vitro and in vivo

金沢大学医学部附属病院泌尿器科Purpose: TAK-165 is a new potent inhibitor of human epidermal growth factor receptor 2 (HER2) tyrosine kinase. Several reports suggest HER2 expression in bladder cancer, renal cell carcinoma (RCC) and androgen-independent prostate cancer. We therefore investigated the antitumor effect of TAK-165 on these urological cancer cells. Materials and methods: Western blot analysis was performed to confirm HER2 expression in cell lines. To study in vitro efficacy, cells were treated with TAK-165 at various concentrations for 72 h and then counted using a hemocytometer. Then the IC50 value was calculated. In the xenograft model, after the tumor reached 200-300 mm3 in volume, mice were orally administered TAK-165 10 mg/kg per day or 20 mg/kg per day or saline for 14 consecutive days (n = 6-8). Results: HER2 expression was observed in HT1376, UMUC3, T24 (bladder), ACHN (kidney), DU145, LNCaP, LN-REC4 (prostate), although the expression level in these cells was weak compared with BT474 (a breast cancer cell line which expresses HER2 strongly). IC50 was varied from 0.09 to greater than 25 μmol/L in the bladder cancer cell line. ACHN cells were less sensitive in vitro. The prostate cancer cell lines studied were all sensitive (IC50 0.053-4.62 μmol/L). In the xenograft model, treatment with TAK-165 significantly inhibited growth of UMUC-3, ACHN, and LN-REC4. The antitumor effect (T/C [%] = growth of TAK-165 treated tumor/average growth of control tumor × 100) after 14 days treatment were 22.9%, 26.0%, and 26.5% in UMUC3, ACHN and LN-REC4, respectively. Conclusions: TAK-165 may be a hopeful new agent for bladder, kidney and androgen-independent prostate cancer

Precise measurement of cross section of 3He(3He,2p)4He by using He-3 doubly charged beam

The fusion cross section of 3He(3He,2p)4He at a center of mass energy of 30 to 50 keV has been measured by using helium-3 doubly ionized beam at a low-energy high current accelerator facility, OCEAN. Free from molecular interference in the beam, the measurement determines the astrophysical S-factor with better statistical and systematical errors than previous data. By using singly and doubly charged helium-3 ions the facility envisages to provide the data from high energy to Gamow energy regions.Comment: 44 pages, 23 Postscript figure