Graft copolymers containing nucleic acid bases and L -Α-amino acids

Graft copolymers of nucleic acid bases and L-Α-amino acids on polyethyleneimine were synthesized, and carboxyl derivatives of adenine and thymine were grafted by the p -nitrophenyl ester method onto linear and branched polyethyleneimine. The carboxyl derivatives of nucleic acid bases were also grafted onto the monomeric graft copolymers of L -Α-amino acids on polyethyleneimine; the carboxyl derivatives were obtained by the condensation of polyethyleneimine with L -Α-amino acids with a coupling agent.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38685/1/170170616_ftp.pd

Graft copolymers of nucleic acid bases on polyethyleneimine: Interaction of the polymers

Interactions of the graft copolymers of nucleic acid bases on polyethyleneimine with polynucleotides were studied. The backbone of the polymer is linear or branched polyethyleneimine. The nucleic acid bases are adenine and thymine. The polynucleotides used were polyadenylic acid (poly-A) and polyuridylic acid (poly-U). Interactions between these polymers were measured by ultraviolet (UV) absorption and the results were compared with the vinyl-type nucleic acid model polymer.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38686/1/170170617_ftp.pd

Attachment of oligonucleotide probes to poly carbodiimide-coated glass for microarray applications

Oligonucleotide-based DNA microarrays are becoming increasingly useful tools for the analysis of gene expression and single nucleotide polymorphisms (SNPs). Here, we present a method that permits the manufacture of microarrays from non-modified oligonucleotides on a poly carbodiimide-coated glass surface by UV-irradiation. The use of UV-irradiation facilitates an increase in the level of signal intensity, but it does not affect signal discrimination by the oligonucleotides immobilized on the surface. The signal intensity obtained for an array fabricated using non-modified oligonucleotides with UV-irradiation is ∼7-fold greater than that without UV-irradiation. The detection of SNPs was tested to ascertain whether this technique could discriminate specific hybridization signals without causing significant UV-irradiation-induced damage to the immobilized oligonucleotides. We found that this immobilization method provides greater hybridization signals and a better match/mismatch ratio of SNPs than do the established aminosilane techniques. Application of this technology to manufacturing DNA microarrays for sequence analysis is discussed