25 research outputs found
The prenylated rab GTPase receptor PRA1.F4 contributes to protein exit from the golgi apparatus
Prenylated Rab acceptor1 (PRA1) functions in the recruitment of prenylated Rab proteins to their cognate organelles. Arabidopsis (Arabidopsis thaliana) contains a large number of proteins belonging to the AtPRA1 family. However, their physiological roles remain largely unknown. Here, we investigated the physiological role of AtPRA1.F4, a member of the AtPRA1 family. A T-DNA insertion knockdown mutant of AtPRA1.F4, atpra1.f4, was smaller in stature than parent plants and possessed shorter roots, whereas transgenic plants overexpressing HA:AtPRA1.F4 showed enhanced development of secondary roots and root hairs. However, both overexpression and knockdown plants exhibited increased sensitivity to high-salt stress, lower vacuolar Na+/K+-ATPase and plasma membrane ATPase activities, lower and higher pH in the vacuole and apoplast, respectively, and highly vesiculated Golgi apparatus. HA:AtPRA1.F4 localized to the Golgi apparatus and assembled into high-molecular-weight complexes. atpra1.f4 plants displayed a defect in vacuolar trafficking, which was complemented by low but not high levels of HA:AtPRA1.F4. Overexpression of HA:AtPRA1.F4 also inhibited protein trafficking at the Golgi apparatus, albeit differentially depending on the final destination or type of protein: trafficking of vacuolar proteins, plasma membrane proteins, and trans-Golgi network (TGN)-localized SYP61 was strongly inhibited; trafficking of TGN-localized SYP51 was slightly inhibited; and trafficking of secretory proteins and TGN-localized SYP41 was negligibly or not significantly inhibited. Based on these results, we propose that Golgi-localized AtPRA1.F4 is involved in the exit of many but not all types of post-Golgi proteins from the Golgi apparatus. Additionally, an appropriate level of AtPRA1.F4 is crucial for its function at the Golgi apparatus. ? 2017 American Society of Plant Biologists. All rights reserved.111Ysciescopu
Single Spin Measurement using Single Electron Transistors to Probe Two Electron Systems
We present a method for measuring single spins embedded in a solid by probing
two electron systems with a single electron transistor (SET). Restrictions
imposed by the Pauli Principle on allowed two electron states mean that the
spin state of such systems has a profound impact on the orbital states
(positions) of the electrons, a parameter which SET's are extremely well suited
to measure. We focus on a particular system capable of being fabricated with
current technology: a Te double donor in Si adjacent to a Si/SiO2 interface and
lying directly beneath the SET island electrode, and we outline a measurement
strategy capable of resolving single electron and nuclear spins in this system.
We discuss the limitations of the measurement imposed by spin scattering
arising from fluctuations emanating from the SET and from lattice phonons. We
conclude that measurement of single spins, a necessary requirement for several
proposed quantum computer architectures, is feasible in Si using this strategy.Comment: 22 Pages, 8 Figures; revised version contains updated references and
small textual changes. Submitted to Phys. Rev.