The Application of XML Key Management Specification in e-Business

The technology of PKI can satisfy most of the needs of security in e_Business. But it is restricted to use the PKI technology adequately because applications can not know the PKI System very well without an uniform architecture standard of PKI system. Now, XKMS gives the standard of key management, so applications can use the underlying PKI system transparently by using the standard interfaces. In this paper, we introduce the XKMS Web Services and the client software development kit (brief as CSDK) developed by ourselves. The web services and the CSDK are all in accord with the XKMS specification.. We also have developed an application system about e_Business to see how to call the web services by using the CSDK

Thermodynamical properties of dark energy with the equation of state ω=ω0+ω1z% \omega =\omega_{0}+\omega_{1}z

The thermodynamical properties of dark energy are usually investigated with the equation of state $\omega =\omega_{0}+\omega_{1}z$. Recent observations show that our universe is accelerating, and the apparent horizon and the event horizon vary with redshift $z$. When definitions of the temperature and entropy of a black hole are used to the two horizons of the universe, we examine the thermodynamical properties of the universe which is enveloped by the apparent horizon and the event horizon respectively. We show that the first and the second laws of thermodynamics inside the apparent horizon in any redshift are satisfied, while they are broken down inside the event horizon in some redshift. Therefore, the apparent horizon for the universe may be the boundary of thermodynamical equilibrium for the universe like the event horizon for a black hole.Comment: 6 pages, 5 figures, Accepted for publication in Physical Review

On equivalence of moduli of smoothness of polynomials in Lp, 0<p⩽∞

AbstractIt is well known that ωr(f,t)p⩽tωr-1(f′,t)p⩽t2ωr-2(f″,t)p⩽⋯ for functions f∈Wpr, 1⩽p⩽∞. For general functions f∈Lp, it does not hold for 0<p<1, and its inverse is not true for any p in general. It has been shown in the literature, however, that for certain classes of functions the inverse is true, and the terms in the inequalities are all equivalent. Recently, Zhou and Zhou proved the equivalence for polynomials with p=∞. Using a technique by Ditzian, Hristov and Ivanov, we give a simpler proof to their result and extend it to the Lp space for 0<p⩽∞. We then show its analogues for the Ditzian–Totik modulus of smoothness ωϕr(f,t)p and the weighted Ditzian–Totik modulus of smoothness ωϕr(f,t)w,p for polynomials with ϕ(x)=1-x2

Genome Editing of \u3cem\u3eWnt-1\u3c/em\u3e, a Gene Associated with Segmentation, via CRISPR/Cas9 in the Pine Caterpillar Moth, \u3cem\u3eDendrolimus punctatus\u3c/em\u3e

The pine caterpillar moth, Dendrolimus punctatus, is a devastating forest pest. Genetic manipulation of this insect pest is limited due to the lack of genomic and functional genomic toolsets. Recently, CRISPR/Cas9 technology has been demonstrated to be a promising approach to modify the genome. To investigate gene functions during the embryogenesis, we introduced CRISPR/Cas9 system in D. punctatus to precisely and effectively manipulate gene expressions inmutant embryos. Compared to controls, knocking out of DpWnt-1, a gene well known for its role in the early body planning, led to high embryonic mortality. Among these mutants, 32.9% of the embryos and larvae showed an abnormal development. DpWnt-1 mutants predominantly exhibited abnormal posterior segments. In addition, multiple phenotypes were observed, including the loss of limbs and the head deformation, suggesting that DpWnt-1 signaling pathway is necessary for anterior segmentation and appendage development. Overall, our results demonstrate that CRISPR/Cas9 system is feasible and efficient in inducing mutations at a specific locus in D. punctatus. This study not only lays the foundation for characterizing gene functions in a non-model species, but also facilitates the future development of pest control alternatives for a major defoliator

Analysis of the Tooth Surface Contact Area of a Circular-Arc-Tooth-Trace Cylindrical Gear under Load

To reconstruct the tooth surface of a circular-arc-tooth-trace cylindrical gear (CATT cylindrical gear), a 3D model has been developed and the contact characteristics have been investigated. Based on the development principle and meshing theory, the tooth surface equation, tooth surface curvature equation and tooth surface contact ellipse equation of the CATT cylindrical gear were deduced, and it was proved that the contact was a point contact. Then, the tooth surface was reconstructed and a 3D model was developed. Next, by performing the finite element analysis and meshing impression experiment, it was proved again that the contact is the point contact, and the contact area became an ellipse under loading. Finally, the influences of the design parameters on the contact ellipse were investigated. The general tendency is that the elliptical contact area increases from the tooth root to the gear top; the elliptical contact area decreases when the modulus and the gear tooth number near the tooth root increase and it increases when the modulus and the gear tooth number near the tooth top increase; the elliptical contact area increases when the tooth line radius increases. The elliptical contact area decreases in a cliff-like manner near the tooth top. The research results provide a reference for the design, profile modification and lubrication of the CATT cylindrical gear

Genome Editing of \u3cem\u3eWnt-1\u3c/em\u3e, a Gene Associated with Segmentation, via CRISPR/Cas9 in the Pine Caterpillar Moth, \u3cem\u3eDendrolimus punctatus\u3c/em\u3e

The pine caterpillar moth, Dendrolimus punctatus, is a devastating forest pest. Genetic manipulation of this insect pest is limited due to the lack of genomic and functional genomic toolsets. Recently, CRISPR/Cas9 technology has been demonstrated to be a promising approach to modify the genome. To investigate gene functions during the embryogenesis, we introduced CRISPR/Cas9 system in D. punctatus to precisely and effectively manipulate gene expressions inmutant embryos. Compared to controls, knocking out of DpWnt-1, a gene well known for its role in the early body planning, led to high embryonic mortality. Among these mutants, 32.9% of the embryos and larvae showed an abnormal development. DpWnt-1 mutants predominantly exhibited abnormal posterior segments. In addition, multiple phenotypes were observed, including the loss of limbs and the head deformation, suggesting that DpWnt-1 signaling pathway is necessary for anterior segmentation and appendage development. Overall, our results demonstrate that CRISPR/Cas9 system is feasible and efficient in inducing mutations at a specific locus in D. punctatus. This study not only lays the foundation for characterizing gene functions in a non-model species, but also facilitates the future development of pest control alternatives for a major defoliator

Implications of C1q/TNF-related protein superfamily in patients with coronary artery disease.

The C1q complement/TNF-related protein superfamily (CTRPs) displays differential effects on the regulation of metabolic homeostasis, governing cardiovascular function. However, whether and how they may serve as predictor/pro-diagnosis factors for assessing the risks of coronary artery disease (CAD) remains controversial. Therefore, we performed a clinical study to elaborate on the implication of CTRPs (CTRP1, CTRP5, CTRP7, and CTRP15) in CAD. CTRP1 were significantly increased, whereas CTRP7 and CTRP15 levels were decreased in CAD patients compared to the non-CAD group. Significant differences in CTRP1 levels were discovered between the single- and triple-vascular-vessel lesion groups. ROC analysis revealed that CTRP7 and CTRP15 may serve as CAD markers, while CTRP1 may serve as a marker for the single-vessel lesion of CAD. CTRP1 and CTRP5 can serve as markers for the triple-vessel lesion. CTRP1 may serve as an independent risk predictor for triple-vessel lesion, whereas CTRP15 alteration may serve for a single-vessel lesion of CAD. CTRP1 may serve as a novel superior biomarker for diagnosis of severity of vessel-lesion of CAD patients. CTRP7, CTRP15 may serve as more suitable biomarker for the diagnosis of CAD patients, whereas CTRP5 may serve as an independent predictor for CAD. These findings suggest CTRPs may be the superior predictive factors for the vascular lesion of CAD and represent novel therapeutic targets against CAD

Differential effects of sulforaphane in regulation of angiogenesis in a co-culture model of endothelial cells and pericytes

Aberrant neovascularization supports nutrients and the oxygen microenvironment in tumour growth, invasion and metastasis. Recapitulation of functional microvascular structures in vitro could provide a platform for the study of vascular conditions. Sulforaphane (SFN), an isothiocyanate, has been reported to possess chemopreventive properties. In the present study, the effects of SFN on cell proliferation and tubular formation have been investigated using endothelial cells (ECs) and pericytes in coculture. SFN showed a dose-dependent inhibition on the growth of ECs and pericytes with IC50 values 46.7 and 32.4 µM, respectively. SFN (5-20 µM) inhibited tube formation in a 3D coculture although a lower dose (1.25 µM) promoted 30% more endothelial tube formation than control. Moreover, SFN affected intercellular communication between ECs and pericytes via inhibition of angiogenic factor such as vascular endothelial growth factor (VEGF) expression in pericytes. However, the expression of its receptor (VEGFR-2) was found significantly increased in ECs. These effects were associated with down-regulation of prolyl hydroxylase domain-containing protein 1 and 2 (PHD1/2) and activation of hypoxia-inducible factor-1 (HIF) pathway by SFN. Furthermore, thioredoxin reductase-1 was also up-regulated by SFN treatment, suggesting that anti-oxidant and redox regulation are involved in angiogenesis. Taken together, the results of this study suggest that SFN differentially regulates endothelial cells and pericytes, and disrupting their interplay through the VEGF-VEGFR signalling pathway. Anti-angiogenesis property of SFN indicates it has potential role as anticancer agent