Application of hepatic image analysis system in assisting preoperative planning for liver surgery

Objective To perform preoperative planning, virtual resection, and risk assessment for patients to be treated by hepatectomy using the liver hepatic image analysis system (IQQA-Liver) and to evaluate the effect and feasibility of three-dimensional (3D) image reconstruction in assisting liver surgery planning. Methods A retrospective analysis was performed on 32 patients who underwent 3D imaging-assisted preoperative planning from July 2012 to July 2014. The full/residual liver volume was calculated; intrahepatic duct reconstruction was performed; the scheme and extent of surgical removal were simulated. The efficacy of reconstruction and the feasibility of surgical plan were evaluated. Results Surgery was completed according to the 3D imaging-assisted preoperative plan in 29 (90.63%) of the 32 patients. After operation, biliary fistula occurred in 1 case, pleural effusion in 3 cases, and ascites in 3 cases. All patients were discharged safely with a mean hospital stay of 13±4 d. Conclusion The 3D image reconstruction of the liver contributes to individualized operation and improves the certainty of surgery though intuitive understanding of the location of the tumor and its anatomic relationship with surrounding vessels and bile ducts or virtual liver surgery

Characteristics and Driving Forces of Non-Grain Production of Cultivated Land from the Perspective of Food Security

A large proportion of the cultivated land in China has been used for non-grain production purposes. As food insecurity is worsening worldwide, this issue has attracted attention from the Chinese government. In order to curb this trend and to ensure food security, this paper explores the quantitative characteristics and spatial distribution of cultivated land used for non-grain purposes in Liyang City, Jiangsu Province, and discusses the clustering characteristics and mechanisms behind this based on spatial autocorrelation analysis and geographically weighted regression (GWR). The results show that most of the cultivated land in Liyang City has not been used for non-grain purposes, and the cultivated land reserve is abundant. Among all land types, irrigable land has the largest non-grain production rate of cultivated land. There is no significant spatial correlation of cultivated land for non-grain purposes in most towns in Liyang, among which Kunlun Street is in the High-High (HH) zone and Daibu Town in the Low-High (LH) zone. It is also found that the same factor has various impacts on the non-grain production of cultivated land in different towns, and the number of enterprises is the core factor that leads to the non-grain use of cultivated land in Liyang city. Low food prices lead some farmers to plant other crops with higher economic benefits, and also lead to the outflow of the rural labor force. This will not only accelerate the non-grain production of cultivated land, but also cause a large amount of cultivated land to be in a state of unmanned cultivation, further aggravating the proportion of non-grain production in cultivated land

Characteristics and Driving Forces of Non-Grain Production of Cultivated Land from the Perspective of Food Security

A large proportion of the cultivated land in China has been used for non-grain production purposes. As food insecurity is worsening worldwide, this issue has attracted attention from the Chinese government. In order to curb this trend and to ensure food security, this paper explores the quantitative characteristics and spatial distribution of cultivated land used for non-grain purposes in Liyang City, Jiangsu Province, and discusses the clustering characteristics and mechanisms behind this based on spatial autocorrelation analysis and geographically weighted regression (GWR). The results show that most of the cultivated land in Liyang City has not been used for non-grain purposes, and the cultivated land reserve is abundant. Among all land types, irrigable land has the largest non-grain production rate of cultivated land. There is no significant spatial correlation of cultivated land for non-grain purposes in most towns in Liyang, among which Kunlun Street is in the High-High (HH) zone and Daibu Town in the Low-High (LH) zone. It is also found that the same factor has various impacts on the non-grain production of cultivated land in different towns, and the number of enterprises is the core factor that leads to the non-grain use of cultivated land in Liyang city. Low food prices lead some farmers to plant other crops with higher economic benefits, and also lead to the outflow of the rural labor force. This will not only accelerate the non-grain production of cultivated land, but also cause a large amount of cultivated land to be in a state of unmanned cultivation, further aggravating the proportion of non-grain production in cultivated land

The Prognostic Significance of miR-21 Expression among Surgically Resected Hepatocellular Carcinoma Patients: Evidence from a Meta-Analysis and Retrospective Cohort Study

Background. To date, microRNA-21 (miR-21) has been reported to be associated with the prognosis of hepatocellular carcinoma (HCC) in various studies, yet the results were inconsistent. The purpose of this two-part study, consisting of a retrospective cohort study and a meta-analysis, sets out to determine the prognostic role of miR-21 expression among HCC patients who underwent surgical resection. Methods. In this study, we first detected miR-21 expression in HCC patients by quantitative real-time PCR (qRT-PCR). Patients were divided into a high miR-21 expression group and a low miR-21 expression group according to the median level of miR-21 expression in tumor tissues. The survival outcomes of the two groups were analyzed by the Kaplan-Meier method with the log-rank test. Multivariate analysis of the prognostic factors was performed with the Cox regression model. Subsequently, eligible studies were obtained by searching on PubMed, Cochrane Library, and Web of Science, and a meta-analysis was performed to assess the prognostic role of miR-21 expression among HCC. Results. The qRT-PCR analysis results of our cohort study showed miR-21 expression was significantly upregulated in HCC tissues when compared with adjacent nontumor tissues. Multivariate analysis suggested that miR-21 expression was an independent prognostic factor for overall survival (OS) (hazard ratio, HR=2.361) and disease-free survival (DFS) (HR=2.024) in HCC patients who underwent surgical resection. A total of 10 studies with 969 patients were enrolled in the meta-analysis, consisting of 9 studies from the database search and our cohort study. We observed that elevated miR-21 expression can predict poor OS (HR=2.24, 95%CI=1.73‐2.91, P<0.001) and DFS/recurrence-free survival (RFS) (HR=2.44, 95%CI=1.62‐3.67, P<0.001) in surgically resected HCC patients. Conclusions. Our study demonstrated that miR-21 high expression among surgically resected HCC patients is a prognostic factor that indicated adverse survival

Prediction and Validation of Hub Genes Associated with Colorectal Cancer by Integrating PPI Network and Gene Expression Data

Although hundreds of colorectal cancer- (CRC-) related genes have been screened, the significant hub genes still need to be further identified. The aim of this study was to identify the hub genes based on protein-protein interaction network and uncover their clinical value. Firstly, 645 CRC patients’ data from the Tumor Cancer Genome Atlas were downloaded and analyzed to screen the differential expression genes (DEGs). And then, the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was performed, and PPI network of the DEGs was constructed by Cytoscape software. Finally, four hub genes (CXCL3, ELF5, TIMP1, and PHLPP2) were obtained from four subnets and further validated in our clinical setting and TCGA dataset. The results showed that mRNA expression of CXCL3, ELF5, and TIMP1 was increased in CRC tissues, whereas PHLPP2 mRNA expression was decreased. More importantly, high expression of CXCL3, ELF5, and TIMP1 was significantly associated with lymphatic invasion, distance metastasis, and advanced tumor stage. In addition, a shorter overall survival was observed in patients with increased CXCL3, TIMP1, and ELF5 expression and decreased PHLPP2 expression. In conclusion, the four hub genes screened by our strategy could serve as novel biomarkers for prognosis prediction of CRC patients

Effect of Boron Content in LiOH Solutions on the Corrosion Behavior of Zr-Sn-Nb Alloy

In pressurized water reactors, LiOH may be concentrated in some areas, leading to the accelerated corrosion of fuel claddings. Injecting boric acid into primary coolants can mitigate the accelerated corrosion effect of LiOH on Zircaloys, but the effects of boron content on the corrosion behavior of the Zr-Sn-Nb alloy are still unknown. This work focused on the corrosion and hydrogen absorption behavior at 360 °C/18.6 MPa in 100 mg/kg LiOH solutions with 0 mg/kg, 50 mg/kg, and 200 mg/kg boron contents for up to 510 days, aiming to study the effect of boron content on corrosion resistance in LiOH solutions. Corrosion kinetics, microstructures of oxide films, hydrogen absorption concentrations and hydride morphology were obtained after the test. The results show that injecting boron in LiOH solutions can significantly reduce the corrosion weight gain, hydrogen concentration, and hydrogen length of Zr-Sn-Nb alloys, that is, improving corrosion resistance effectively. During the oxidation of the Zr-Sn-Nb alloy, B3+ and Li+ incorporate in oxide films. The incorporation of Li+ may lead to the generation of oxygen vacancies, which can carry oxygen from the solutions to O/M interface, accelerating corrosion. The incorporation of B3+ in oxide films will slow down the oxidation of Zr-Sn-Nb alloys by reducing the oxygen vacancies caused by Li+ aggregation

Profiles of alternative splicing in colorectal cancer and their clinical significance: A study based on large-scale sequencing dataResearch in Context

Background: Alternative splicing (AS), as a potent and pervasive mechanism of transcriptional regulatory, expands the genome's coding capacity and involves in the initiation and progression of cancer. Systematic analysis of alternative splicing in colorectal cancer (CRC) is lacking and greatly needed. Methods: RNA-Seq data and corresponding clinical information of CRC cohort were downloaded from the TCGA data portal. Then, a java application, known as SpliceSeq, was used to evaluate the RNA splicing patterns and calculate the Percent Spliced In (PSI) value. Differently expressed AS events (DEAS) were identified based on PSI value between paired CRC and adjacent tissues. DEAS and its splicing networks were further analyzed by bioinformatics methods. Kaplan-Meier, Cox proportional regression and unsupervised clustering analysis were used to evaluate the association between DEAS and patients' clinical features. Results: After strict filtering, a total of 34,334 AS events were identified, among which 421 AS events were found expressed differently. Parent genes of these DEAS play a important role in regulating CRC-related processes such as protein kinase activity (FDR<0.0001), PI3K-Akt signaling pathway (FDR = 0.0024) and p53 signaling pathway (FDR = 0.0143). 37 DEAS events were found to be associated with OS, and 68 DEAS events were found to be associated with DFS. Stratifying patients according to the PSI value of AT in CXCL12 and RI in CSTF3 formed significant Kaplan-Meier curves in both OS and DFS survival analysis. Unsupervised clustering analysis using DEAS revealed four clusters with distinct survival patterns, and associated with consensus molecular subtypes. Conclusions: Large differences of AS events in CRC appear to exist, and these differences are likely to be important determinants of both prognosis and biological regulation. Our identified CRC-related AS events and uncovered splicing networks are valuable in deciphering the underlying mechanisms of AS in CRC, and provide clues of therapeutic targets to further validations. Keywords: CRC, Alternative splicing, RNA-Seq, Prognosi

Advances in nanomedicines for lymphatic imaging and therapy

Abstract Lymph nodes play a pivotal role in tumor progression as key components of the lymphatic system. However, the unique physiological structure of lymph nodes has traditionally constrained the drug delivery efficiency. Excitingly, nanomedicines have shown tremendous advantages in lymph node-specific delivery, enabling distinct recognition and diagnosis of lymph nodes, and hence laying the foundation for efficient tumor therapies. In this review, we comprehensively discuss the key factors affecting the specific enrichment of nanomedicines in lymph nodes, and systematically summarize nanomedicines for precise lymph node drug delivery and therapeutic application, including the lymphatic diagnosis and treatment nanodrugs and lymph node specific imaging and identification system. Notably, we delve into the critical challenges and considerations currently facing lymphatic nanomedicines, and futher propose effective strategies to address these issues. This review encapsulates recent findings, clinical applications, and future prospects for designing effective nanocarriers for lymphatic system targeting, with potential implications for improving cancer treatment strategies